您的位置：首頁 > 技術文獻 > 產品說明 > Anti-NLRP3 antibody Catalog Number：OM641721
| Product Name | Anti-NLRP3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1 mg/ml |
| Purification | Protein A |
| Gene Symbol | NLRP3 |
|---|---|
| Gene Synonyms | More |
| Gene Full Name | NLR family pyrin domain containing 3 |
| Gene Summary | This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, ａnd a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, ａnd is a member of the NLRP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, ａnd it plays a role in the regulation of inflammation, the immune response, ａnd apoptosis. The SARS-CoV 3a protein, a transmembrane pore-forming viroporin, has been shown to activate the NLRP3 inflammasome via the formation of ion channels in macrophages. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous ａnd articular (CINCA) syndrome, neonatal-onset multisystem inflammatory disease (NOMID), keratoendotheliitis fugax hereditarian, ａnd deafness, autosomal dominant 34, with or without inflammation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5＇ UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5＇ UTR splice patterns are biologically valid. [provided by RefSeq, Aug 2020] More |
| Molecular Weight(MW) | 118 kDa |
| Function | As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity ａnd inflammation. In response to pathogens ａnd other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD ａnd CASP1 (and possibly CASP4 ａnd CASP5). Recruitment of proCASP1 to the inflammasome promotes its activation ａnd CASP1-catalyzed IL1B ａnd IL18 maturation ａnd secretion in the extracellular milieu More |
| Cellular Localization | Cytosol;Extracellular region or secreted;Endoplasmic reticulum;Nucleus |
Western blot analysis using NLRP3 antibody against Hep G2 (1), K562 (2) cell lysate.
Immunohistochemical analysis of paraffin-em
Immunofluorescence analysis of NIH/3T3 cells using NLRP3 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
| Application Notes | WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
① 凡本網注明"來源：易推廣"的所有作品，版權均屬于易推廣，未經本網授權不得轉載、摘編或利用其它方式使用。已獲本網授權的作品，應在授權范圍內
使用，并注明"來源：易推廣"。違者本網將追究相關法律責任。② 本信息由注冊會員：上海篤瑪生物科技有限公司 發布并且負責版權等法律責任。
