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| Product Name | HLA-G Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB~~1:100~500 IHC~~1:10~50 FC~~1:10~50: | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
| Purification |
| Gene Synonyms | |
|---|---|
| Alternative Names | More |
| Molecular Weight(MW) | 38224 Da |
| Function | Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages ａnd mononuclear cells |
| Tissue Specificity | Expressed in trophoblasts. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 3135 |
|---|
Western blot analysis of HLA-G Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). HLA-G (arrow) was detected using the purified Pab.
Western blot analysis of HLA-G (arrow) using rabbit polyclonal HLA-G Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-G gene.
Formalin-fixed ａnd paraffin-embedded human lung carcinoma reacted with HLA-G Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
HLA-G Antibody (Center) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell ( histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
| Application Notes | WB~~1:100~500 IHC~~1:10~50 FC~~1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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