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              您的位置:首頁 > 技術文獻 > 產品說明 > HSPA5 Antibody (C-term) Catalog Number:OM235481

              標題HSPA5 Antibody (C-term) Catalog Number:OM235481

                 

              提供者:上海篤瑪生物科技有限公司    發布時間:2024/8/30   閱讀次數:35次 >>進入該公司展臺
                

              Product Profile

              Product Name HSPA5 Antibody (C-term)
              Antibody Type Primary Antibodies

              Key Feature

              Clonality Polyclonal
              Isotype Ig
              Host Species Rabbit
              Tested Applications

              WBIHCFC

               
              WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50:
              Species Reactivity

              Human

              Concentration 1 mg/ml
              Purification  

              Target Information

              Gene Synonyms
              GRP78
              Alternative Names
              HSPA5
              GRP78
              78 kDa glucose-regulated protein
              Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
              Heat shock 70 kDa protein 5
              Immunoglobulin heavy chain-binding protein
              More 
              Molecular Weight(MW) 72333 Da
              Function
              Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER
              Tissue Specificity This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 548-587 amino acids from the C-terminal region of human HSPA5.
              Cellular Localization Endoplasmic reticulum lumen. Melanosome. Cylasm (By similarity). Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV

              Database Links

              Entrez Gene 3309
              Nucleotide Accession NP_005338

              Application

              • Application

                HSPA5 Antibody (C-term) western blot analysis in A375,A2058,Hela cell line lysates (35ug/lane).This demonstrates the HSPA5 antibody detected the HSPA5 protein (arrow).

              • Application

                HSPA5 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSPA5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

              • Application

                HSPA5 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

              Application Notes
              WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50:

              Additional Information

              Form Liquid
              Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
              Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

              關鍵詞:穩定性強  特異性強    靈敏度高  

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