一般描述
The IL6 (interleukin-6) gene is mapped to human chromosome 7p15.3.
[1] All the members of IL6 family have membrane glycoprotein gp130 as a common receptor.
[2] IL6 (interleukin-6) induces B-cell differentiation ａnd is a primary regulator of inflammation.
[3]應用
Interleukin-6 human has been used to check its role in breast cancer metastasis.
[4] It has also been used for evaluating its role in D5HS tumor sphere formation.
[5]生化/生理作用
IL6 regulates tumor proliferation ａnd inhibits apoptosis.
[3] It contributes to the disease progression of coronary artery disease.
[6]白細胞介素-6 (IL-6) 是初在雙鏈 RNA 刺激的細胞培養基中發現的一種多功能蛋白。IL-6 似乎直接參與了感染和損傷后發生的反應，并可能證明在調節急性期反應方面與 IL-1 和 TNF-α 同樣重要。據報道，IL-6 由成纖維細胞、活化的 T 細胞、活化的單核細胞或巨噬細胞以及內皮細胞產生。作用于多種細胞，包括成纖維細胞、髓系祖細胞、T 細胞、B 細胞和肝細胞。如許多同義詞所示，IL-6 誘導多種效應：漿細胞瘤生長因子 (PCT-GF)、干擾素-β-2（IFN-β 2 ）、單核細胞來源的人 B 細胞生長因子、B 細胞刺激因子 (BSF-2)、肝細胞刺激因子 (HSF)、白細胞介素雜交瘤/漿細胞瘤-1 (IL-HP1)。此外，IL-6 似乎在 T 淋巴細胞增殖中與 IL-2 相互作用。IL-6 還可增強 IL-3 對多潛能造血祖細胞的增殖作用。
制備說明
Human IL-6, expressed in human 293 cells, is a glycosylated monomer with an apparent molecular mass of 26-30 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media ａnd other applications.
分析說明
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
