1.購買我公司任意ELISA試劑盒指標享受免費代測，并提供技術指導服務！

二、實驗材料與試劑配制：

1. 儀器與材料：酶標儀（使用前預熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時備用（如果標本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人過氧化物酶體增殖激活物受體γ輔激活因子1α(PPARGC1)ELISA試劑盒廠家】三. 仔細閱讀說明書

1.檢查試劑盒標簽上的有效期。如果超過有效期，請勿使用。

2. 按說明書確定所有試劑齊全（數量、體積）

3. 標本制備要規范，每份標本體積要按2-3個復孔以上的量制備（貯存），盡量分裝做備份。

4.短期內無法實驗者，注意低溫保存

5.準備好所有實驗額外所需物品，（比如移液器，試管，清洗器，酶標儀）

6. 按說明書將所用試劑平衡至室溫，根據檢測標本數量確定所需試劑的量

7.檢查試劑盒內每種試劑的貯存條件，保證所有試劑均按照試劑盒內說明書推薦的條件存儲。 【人過氧化物酶體增殖激活物受體γ輔激活因子1α(PPARGC1)ELISA試劑盒廠家】

8.檢查不穩定或者變質的試劑溶液（比如沉淀或變色）。有些試劑，比如標準品稀釋液或者檢測稀釋液，可能在設計時就含有沉淀物。所有試劑在滴入酶標板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標板之前，必需不停攪拌。

9.保證充分的孵育時間和溫度。

10.切勿使用不同生產批號的試劑取代現有試劑或混合現有試劑。

11.在混合或溶解蛋白溶液時，避免泡沫產生。

12.在實驗開始之前，安排好實驗流程。在實驗開始之前，清潔工作臺。

13.若有問題，應與我公司或代理商的技術支持聯系

【人過氧化物酶體增殖激活物受體γ輔激活因子1α(PPARGC1)ELISA試劑盒廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩定。我們提倡新鮮標本盡早檢測，對收集后當天就進行檢測的標本，及時儲存在4℃備用，如有特殊原因需要周期收集標本，請造模取材后，將標本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質量，所以避免反復凍融。部分標本也可加入適當防腐劑，激素類標本需添加抑肽酶。 

2.試劑盒代測標本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項請與我司技術人員溝通。

3.液體類標本：標本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細胞培養上清、組織勻漿等。

4.購買前請提前告訴我們：您樣本的種屬、樣本數量、待測指標及其它特殊要求。

【人過氧化物酶體增殖激活物受體γ輔激活因子1α(PPARGC1)ELISA試劑盒廠家】五、代測數據詳細內容

1、ELISA標準曲線；

2、實驗具體詳細步驟；

3、完整的實驗報告（試劑耗材，實驗方法，實驗結果及結果說明）；

4、實驗進行階段，我公司客服人員會及時向您匯報實驗進程。

9237106  Protocol Dev./Opt., on-site (2-days)         The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed.          CNY                             

9237097  Training, BR QIAGEN Advanced       A minimum of 4 people are required to attend. The QIAGEN BioRobot Systems Advanced Training includes three days of  training at our training facility to introduce users to advanced software topics for the  QIAGEN BioRobot Systems.  Designed for users with a basic understanding of the use ａnd programming of QIAGEN BioRobot systems who want to write complex protocols for their instrument, the advanced training starts with a brief review of basic concepts such as teaching slots ａnd racks before exploring the higher-level programming features of the QIAsoft Operating System.  Topics include the structure ａnd use of functions, using variable for programming flexibility, IF/ELSE/END boolean logic commands, ａnd parameterized pipetting using tables.  Users will write, test ａnd run protocols they have developed to demonstrate ａnd reinforce training success.       CNY                             

9236422  Power supply, replacement kit, TWI                  CNY                             

9232968  Print YZ96-Adapter-Board Plato-7            CNY                             

9236363  Reservoir XYP            CNY                             

9236353  Tubing Sample Needle to 3-Way Valve              CNY                             

9236329  2-Way Valve (Sheath or vent)           CNY                              【人過氧化物酶體增殖激活物受體γ輔激活因子1α(PPARGC1)ELISA試劑盒廠家】

9236160  Potentionmeter, rotary, kit, RapidPlate           CNY                             

9236154  Online Fill Pump RP           CNY                             

9236126  QIAGEN Multiwell-Plate Holder , silver   Holder for accommodating multiwell plates on the Microplate station of the BioRobot MDx worktable         CNY                             

9236120  Runner, Drawer, BR8 RD                   CNY                             

9235790  Ramp, Locking, Drawer, BR8 RD              CNY                             

9230745  Z-Lead screw for Plato 7           CNY                             

9235690  Cable X-Main, RCS1           CNY                             

9235572  Shaker Tool, RCS1              CNY                             

9230314  Cable, RS232, syringe pump, 225mm                CNY                             

9230283  Cable, Interface, X-main to Washer                   CNY                             

5000-1302        Capacitor 2UF, SKR1                  CNY                             

5000-1284        DML 2000 - Power Cord, 220V D Type (GB)              CNY                             

5000-1254        Power Supply PCB, DML2                  CNY                             

5000-1250        CPU DML 2000 ASSRY Kit, DML2              CNY                             

6000-3401        RCS Upgrade Package               CNY                             

6000-3116        Plate Washer I - 105 Micron Filters          CNY                              【人過氧化物酶體增殖激活物受體γ輔激活因子1α(PPARGC1)ELISA試劑盒廠家】

6000-00183      Plate Washer - Manifold           CNY                             

6000-00177      Plate Washer - Syringe Assembly              CNY                             

5000-1501        Holder Fuse, HTR1            CNY                             

5135-1220        hc2 CT-ID DNA Test           CNY                             

BR-01       DxS B-RAF Mutation Test Kit            CNY                             

9240716  Lead Screw Assy, Capper U\D, AP196/98          CNY                             

9240712  Muffler, Elevator Brake, AP196/98            CNY                             

5000-1416        Switch LRGSK61RCBE250, VTX2                CNY                             

6000-5023        LumiCheck Plate User Package (CD Manual)            CNY                             

6000-3102        Rapid Capture System & Manuals            CNY                             

MCM        小鼠心肌細胞         Mouse Cardiac Myocytes         1 x 10^6 cells/vial                                

MCF 小鼠心臟成纖維細胞     Mouse Cardiac Fibroblasts       5 x 10^5 cells/vial                              

MMSC-bm        小鼠骨髓間充質干細胞         Mouse Mesenchymal Stem Cells-bone marrow       5 x 10^5 cells/vial                                

MEF-mt    小鼠胚胎成纖維細胞     Mouse Embryonic Fibroblasts 5 x 10^5 cells/vial                                 【人過氧化物酶體增殖激活物受體γ輔激活因子1α(PPARGC1)ELISA試劑盒廠家】

RPC  大鼠垂體細胞         Rat Pituitary Cells    5 x 10^5 cells/vial                                

RMC          大鼠腦膜細胞         Rat Meningeal Cells          5 x 10^5 cells/vial                                

RN-r 大鼠腦脊神經元     Rat Neurons-raphe  1 x 10^6 cells/vial                                

RN-c          大鼠皮質神經元     Rat Neurons-cortical        1 x 10^6 cells/vial                                

RGC 大鼠顆粒細胞         Rat Granule Cells     1 x 10^6 cells/vial                                

RN-h         大鼠海馬趾神經元         Rat Neurons-hippocampal       1 x 10^6 cells/vial                                

RN-sn        大鼠黑質神經元     Rat Neurons-substantia nigra 1 x 10^6 cells/vial                                

RN-s 大鼠紋狀體神經元         Rat Neurons-striatal         1 x 10^6 cells/vial                                

RN-vsc      大鼠腹脊髓神經元         Rat Neurons-ventral spinal cord      1 x 10^6 cells/vial                                

RN-dsc      大鼠背脊髓神經元         Rat Neurons-dorsal spinal cord        1 x 10^6 cells/vial                                

RN-sc        大鼠背脊髓神經元         Rat Neurons-dorsal spinal cord        1 x 10^6 cells/vial                              

ROPC        大鼠少突膠質前體細胞         Rat Oligodendrocyte Precursor Cells        1 x 10^6 cells/vial                                

ROPC-f      大鼠少突膠質前體細胞-新鮮        Rat Oligodendrocyte Precursor Cells-fresh      1 x 10^6 cells/tube                                     

RSC  大鼠雪旺細胞         Rat Schwann Cells    5 x 10^5 cells/vial                                

RPNF         大鼠神經束膜細胞         Rat Perineural Fibroblasts        5 x 10^5 cells/vial                                

RA    大鼠星形膠質細胞         Rat Astrocytes 1 x 10^6 cells/vial                                 【人過氧化物酶體增殖激活物受體γ輔激活因子1α(PPARGC1)ELISA試劑盒廠家】

RA-c 大鼠小腦星形膠質細胞         Rat Astrocytes-cerebellar        1 x 10^6 cells/vial                                

RA-h          大鼠海馬趾星形膠質細胞     Rat Astrocytes-hippocampal   1 x 10^6 cells/vial                                

RM   大鼠小膠質細胞     Rat Microglia   5 x 10^5 cells/vial                                

RMa-bm  大鼠巨噬細胞         Rat Macrophage      1 x 10^6 cells/vial                              

REK-n        大鼠表皮角質細胞-新生        Rat Epidermal Keratinocytes-neonate    5 x 10^5 cells/vial                                

RDF-n       大鼠皮膚成纖維細胞 - 新生        Rat Dermal Fibroblasts - neonate   5 x 10^5 cells/vial                                

RDF-a       大鼠皮膚成纖維細胞-成年   Rat Dermal Fibroblasts-adult  5 x 10^5 cells/vial                                

RLF   大鼠淋巴成纖維細胞     Rat Lymphatic Fibroblasts        5 x 10^5 cells/vial                                

RPAEpiC   大鼠肺泡上皮細胞         Rat Pulmonary Alveolar Epithelial Cells   1 x 10^6 cells/vial                                

RPF  大鼠肺成纖維細胞         Rat Pulmonary Fibroblasts       5 x 10^5 cells/vial                                

RRTEpiC   大鼠腎小管上皮細胞     Rat Renal Tubular Epithelial Cells    5 x 10^5 cells/vial