【友情提示】：本產品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準備試劑，樣品和標準品

2.加入準備好的樣品和標準品，37℃反應30分鐘

3.洗板5次，加入酶標試劑，37℃反應30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內讀OD值

7.計算
齊一生物供應ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據。為了保證實驗數據的可靠性，在實驗過程中必須堅持全面質量控制和全過程質量控制。在收集標本前都必須有一個完整的計劃。

【人基質金屬蛋白酶11(MMP11)ELISA試劑盒現貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【人基質金屬蛋白酶11(MMP11)ELISA試劑盒現貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標

              1g組織加入1ml組織裂解液加入8ml PBS 測細胞內

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【人基質金屬蛋白酶11(MMP11)ELISA試劑盒現貨】樣本離心轉速及時間

1.試管采集的樣本：3000轉，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應將其分成小部分-70 ℃保存，避免反復冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應在室溫下解凍并確保樣品均勻地充分解凍。

【人基質金屬蛋白酶11(MMP11)ELISA試劑盒現貨】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

QY-00070       AM Adrenomedullin 腎上腺髓質素（抗原）             0.1mg/1800                       

QY-04160       TIMP-2(Tissue Inhibitor of Metalloproteinase-2)金屬蛋白酶組織抑制因子-2(抗原)              0.5mg/6501mg/1100                           

QY-04170       TIMP-3(Tissue Inhibitor of Metalloproteinase-3)金屬蛋白酶組織抑制因子(抗原)          0.5mg/6501mg/1100                         

QY-00900       ADM R, Adrenomedullin receptor腎上腺髓質素受體（抗原）         0.5mg/6501mg/1100                         

Yf-01580 Amylin1-37/FITC異硫氰酸熒光素(FITC)標記糖尿病相關肽1-37          1ml/2860                           

QY-03060       VTG(vitellogenin)青鳉魚卵黃蛋白              0.1mg/6500.2mg/1100                      

QY-00580       Apaf-1 （Apoptosis protease activating factor-1）(CT)凋亡蛋白活性因子-1（抗原）          0.5mg/7001mg/1200                            【人基質金屬蛋白酶11(MMP11)ELISA試劑盒現貨】

QY-00590       Apaf-1 （Apoptosis protease activating factor-1）(NT)凋亡蛋白活性因子-1（抗原）          0.5mg/7001mg/1200                           

QY-01180       AR （Androgen Receptor)雄激素受體（抗原）               0.5mg/6501mg/1100                         

QY-00450       AT （Angiotenogen）血管緊張素原（抗原）           0.5mg/6501mg/1100                         

QY-01670       Apo-E （Apolipoprotein E）載脂蛋白E（抗原）             0.5mg/6501mg/1100                         

QY-02450       IKI3 family protein         0.5mg/8001mg/1200                         

QY-03170       ILK-1 (Integrin-linked protein kinase 1)整合素連接激酶-1         0.5mg/6501mg/1100                         

QY-01140       Aromatase芳香化酶（抗原）             0.5mg/6501mg/1100                         

QY-00550       ART(Artemin)（抗原）         0.5mg/6501mg/1100                         

QY-02610       AQP-2 （aquaporin Protein-2）水通道蛋白-2（抗原）           0.5mg/6501mg/1100                         

QY-01640       BACE( ASP2 ） β分泌酶（抗原）           0.5mg/6501mg/1100                         

QY-02750       Bassoon(RCN)               0.5mg/6501mg/1200                         

QY-01270       Bax （Bcl-2 Assaciated X protein） Bax（多肽抗原）             0.5mg/6501mg/1100                         

QY-00320       Bcl-2（抗原）Bcl-2（多肽抗原）              0.5mg/6501mg/1100                         

QY-02480       BDNF (Brain-derived Neurotrophin factor)腦源神經營養因子(腦衍化神經營養因子)            0.5mg/6501mg/1100                           

QY-02170       bFGF堿性成纖維細胞生長因子（多肽抗原）          0.5mg/6501mg/1100                         

QY-00440       BNP （Brain Natriuretic Peptide ）腦鈉素（多肽抗原）         0.5mg/6501mg/1100                          【人基質金屬蛋白酶11(MMP11)ELISA試劑盒現貨】

QY-04890       BrdU (Bromodeoxyuridine)溴脫氧尿苷               50mg/100100mg/1501g/1300                          

QY-03240       C5過敏毒素C5（補體C5）抗原               0.5mg/6501mg/1100                         

QY-00910       CA125 （Ovarian cancer antigen）卵巢癌抗原（抗原）          0.5mg/6501mg/1100                         

QY-00430       CAD（Caspase-Activated Deoxyribonuclease）Caspase 激活的脫氧核糖核酸酶（抗原）           0.5mg/6501mg/1100                         

QY-00950       Calponin 1鈣調節蛋白-1（抗原）              0.5mg/6501mg/1100                         

QY-01690       Caspase-1 (ICE; CASP-1；P45)天冬氨酸-胱氨酸特異性蛋白酶家族（抗原）             0.5mg/6501mg/1100                         

QY-00810       Caspase-3 （CPP32）半胱胺酸蛋白酶蛋白-3（抗原）           0.5mg/6501mg/1100                         

QY-00840       Caspase-6 （NT）半胱胺酸蛋白酶蛋白-6（抗原）         0.5mg/6501mg/1100                         

QY-01510       Caspase-6 （CT）半胱胺酸蛋白酶蛋白-6（抗原）         0.5mg/6501mg/1100                         

QY-00490       Caspase-9白介素1-β轉化酶樣凋亡蛋白酶6（抗原）           0.5mg/6501mg/1100                         

9001946  QIAcube (220-240V) Promo        CNY                           

971061    therascreen® MGMT Pyro Kit (48)             CNY                           

293035    mericon Apricot kernels (96)        CNY                           

929520    QX Alignment Marker 15bp/500bp (1.5 ml)         CNY                           

9012643  Bottle, system liquid              CNY                           

9012596  Disposable Tips, 300µl (960) Conducting disposable tips    CNY                           

133121    NeXtalStock Lithium citrate  (200)            CNY                           

133119    NeXtalStock Ammonium citrate  (200)              CNY                           

133190    NeXtalStock D-(+)-Glucose (200)              CNY                           

51162      QIAamp 96 DNA Blood Kit (12)    For 12 x 96 DNA preps: 12 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels  CNY                           

50112      QIAamp UCP PurePathogen Blood Kit (10)         CNY                           

133104    NeXtalStock Sodium acet.  (200)              CNY                           

9015468  Sensor, photo, PM-L24         CNY                           

9001871  EZ1 Advanced, System  EZ1 Advanced, System combines the EZ1 Advanced system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with a training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                           

9018458  Screw, w finishing washer (4), QX             CNY                           

9018447  Purge Tool, cartridge, QX            CNY                            【人基質金屬蛋白酶11(MMP11)ELISA試劑盒現貨】

9012412  Incubator Tower (65°) for Plato 3 hold        CNY                           

9012392  Bottle, waste, 10 l, BR8000          CNY                           

205111    Omniscript RT Kit (50)  For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water      CNY                           

9018425  Door, sample, QX         CNY                           

9002123  QIAxcel Advanced System    QIAxcel Advanced System combines the QIAxcel Advanced instrument ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                           

57714     QIAamp MinElute Virus Vacuum Kit (50)     For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, Carrier RNA, Buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)     CNY                           

972012    PyroMark Q96 CpG p16 (96)       For quantification of methylation level in p16       CNY                           

133227    NeXtalStock Sodium cacodyl. pH 6.0 (100)        CNY                           

301108    PolyFect Transfection Reagent (100 ml)      For 2500-6500 transfections in 60 mm dishes or 5000-10,000 transfections in 6-well plates.  CNY                           

56304     QIAamp DNA Micro Kit (50) For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)  CNY                           

9002031  Rotor-Gene Q MDx 5Plex HRM no PC        CNY                           

9002018  Rotor-Gene Q MDx 2plex HRM, Priority     Rotor-Gene Q 2plex HRM, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents. CNY                           

970902    PyroMark Q24 Advanced Kit (4x24)            CNY                           

133211    NeXtalStock Magnesium chloride H2O (200)             CNY                           

133195    NeXtalStock Sodium tartrate pH 5.6 (200)          CNY