【友情提示】：本產品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準備試劑，樣品和標準品

2.加入準備好的樣品和標準品，37℃反應30分鐘

3.洗板5次，加入酶標試劑，37℃反應30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內讀OD值

7.計算
齊一生物供應ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據。為了保證實驗數據的可靠性，在實驗過程中必須堅持全面質量控制和全過程質量控制。在收集標本前都必須有一個完整的計劃。

【人preptinELISA試劑盒現貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【人preptinELISA試劑盒現貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標

              1g組織加入1ml組織裂解液加入8ml PBS 測細胞內

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【人preptinELISA試劑盒現貨】樣本離心轉速及時間

1.試管采集的樣本：3000轉，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應將其分成小部分-70 ℃保存，避免反復冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應在室溫下解凍并確保樣品均勻地充分解凍。

【人preptinELISA試劑盒現貨】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

QR20-74 Taq DNA Polymerase     1000U                                

QR20-75 2 x Taq MasterMix 1ml                             

QR20-76 2 x Taq MasterMix 3ml                             

QR20-77 Pfu DNA Polymerase     250U                                  

QR20-78 Pfu DNA Polymerase     500U                                  

QR20-79 2 x Pfu MasterMix 1ml                             

QR20-80 2 x Pfu MasterMix 3ml                              【人preptinELISA試劑盒現貨】

QR20-81 HotStar Taq DNA Polymerase       125U                                  

QR20-82 HotStar Taq DNA Polymerase       500U                                  

QR20-83 2 x HotStar Taq MasterMix   1ml                             

QR20-84 2 x HotStar Taq MasterMix   3ml                             

QR20-85 Oligo(dT)15    500μl(10μM)                             

QR20-86 M-MLV反轉錄酶  10000U                              

QR20-87 M-MLV反轉錄酶  50000U                              

QR20-88 M-MLV cDNA鏈合成試劑盒      20次                                 

QR20-89 M-MLV cDNA鏈合成試劑盒      50次                                 

QR20-90 Thermo M-MLV Reverse Transcriptase       10000U                              

QR20-91 Thermo M-MLV Reverse Transcriptase       50000U                              

QR20-92 Thermo M-MLV cDNA鏈合成試劑盒 20次                                 

QR20-93 Thermo M-MLV cDNA鏈合成試劑盒 50次                                 

QR20-94 實時熒光定量 PCR MasterMix    1ml                             

QR20-95 實時熒光定量 PCR MasterMix    3ml                             

QR20-96 RealSuper二步法熒光定量PCR試劑盒     20次                                  【人preptinELISA試劑盒現貨】

QR20-97 RealSuper二步法熒光定量PCR試劑盒     100次                                

QR20-98 pUX-T TA 克隆試劑盒 20次                                 

QR20-99 pUX-T simple TA 克隆試劑盒     20次                                 

QR20-100      加A試劑盒   20次                                 

QR20-101      T4連接酶      200U                                  

QR20-102      0感受態細胞       10 x 100μl                               

QR20-103      0感受態細胞       20 x 100μl                               

QR20-104      DH5α感受態細胞 10 x 100μl                               

QR20-105      DH5α感受態細胞 20 x 100μl                               

QR20-106      BL21(DE3)感受態細胞 10 x 100μl                               

9240948  Cable, x, portal, Qsym          CNY                           

9240825  Field Upgrade, BR EZ1 DSP         CNY                           

9240817  #Premium Cover Agreement EZ1 DSP, 1YR "Repair service delivery: onsite

Service response time (repair): 24 working hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"      CNY                           

9240776  Dispense Head Update, AP96        CNY                           

9022763  EZ1 Adv. XL DNA Invest. Flip-Cap Card            CNY                           

6000-5012      Lumicheck Battery Replacement Package            CNY                           

6000-5005      Micro Well Strips          CNY                           

9241774  QIAgility, Installation     The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the QIAgility systems hardware ａnd trains the user on the operation of the software. After the training the user will be able to run ａnd install application programs ａnd conduct a basic troubleshooting. All travel ａnd labor costs are included. The service is documented in accordance with GMP/GLP requirements.   CNY                           

9241655  Input Rack Pins Kit, AP        CNY                           

9241360  Plastic supporters, door hinge, EZ1a            CNY                           

9243547  Pyro Q24, PM Service   "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY                           

9243112  Hard Drive, Programmed, Cheetah, AP98            CNY                           

9242770  Belt, x-drive, portal, AS, Qsym            CNY                           

9242698  RGQ, Full Agreement     "Repair service delivery: send to Regional Repair Center

Service response time (repair): 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: yes, included (provision time 2 days)

Transportation cost coverage: yes, included"       CNY                           

5000-1406      Control PCB 120V, VTX2             CNY                           

5000-1320      Label Front Overlay, SKR1           CNY                            【人preptinELISA試劑盒現貨】

5000-1312      Platform, SKR1             CNY                           

5000-1274      Plate Mask, DML2        CNY                           

5000-1253      Iris Control PCB, DML2              CNY                           

5050-1071 V.1       CD RomManual-DML2000 Instrument I, 240V          CNY                           

5050-00031    CONVERTER USB        CNY                           

5000-1509      Control Heater, HTR1           CNY                           

6000-3121      Plate Washer 1 - Replacement Tubing Kit            CNY                           

6000-3115      Plate Washer I - Manifold Accessory Kit             CNY                           

5000-1502      Fuse 250V 1A, HTR1           CNY                           

5000-1419      Breaker Circuit 1Pole, VTX2        CNY                           

5198-1220      hc2 HPV DNA Test(High Risk ａnd Low Risk)            CNY                           

EA10224 EASYartus VZV RG_II         CNY                           

EA10023 EASYartus HSV-1/2 RG_I            CNY                           

PK-01     Mutation Test Kit (25)           CNY                           

9240744  Belt, Reagent Pump , Long, AP96         CNY