1.購買我公司任意ELISA試劑盒指標享受免費代測，并提供技術指導服務！

二、實驗材料與試劑配制：

1. 儀器與材料：酶標儀（使用前預熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時備用（如果標本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【雞神經膠質纖維酸性蛋白(GFAP)ELISA試劑盒廠家】三. 仔細閱讀說明書

1.檢查試劑盒標簽上的有效期。如果超過有效期，請勿使用。

2. 按說明書確定所有試劑齊全（數量、體積）

3. 標本制備要規范，每份標本體積要按2-3個復孔以上的量制備（貯存），盡量分裝做備份。

4.短期內無法實驗者，注意低溫保存

5.準備好所有實驗額外所需物品，（比如移液器，試管，清洗器，酶標儀）

6. 按說明書將所用試劑平衡至室溫，根據檢測標本數量確定所需試劑的量

7.檢查試劑盒內每種試劑的貯存條件，保證所有試劑均按照試劑盒內說明書推薦的條件存儲。 【雞神經膠質纖維酸性蛋白(GFAP)ELISA試劑盒廠家】

8.檢查不穩定或者變質的試劑溶液（比如沉淀或變色）。有些試劑，比如標準品稀釋液或者檢測稀釋液，可能在設計時就含有沉淀物。所有試劑在滴入酶標板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標板之前，必需不停攪拌。

9.保證充分的孵育時間和溫度。

10.切勿使用不同生產批號的試劑取代現有試劑或混合現有試劑。

11.在混合或溶解蛋白溶液時，避免泡沫產生。

12.在實驗開始之前，安排好實驗流程。在實驗開始之前，清潔工作臺。

13.若有問題，應與我公司或代理商的技術支持聯系

【雞神經膠質纖維酸性蛋白(GFAP)ELISA試劑盒廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩定。我們提倡新鮮標本盡早檢測，對收集后當天就進行檢測的標本，及時儲存在4℃備用，如有特殊原因需要周期收集標本，請造模取材后，將標本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質量，所以避免反復凍融。部分標本也可加入適當防腐劑，激素類標本需添加抑肽酶。 

2.試劑盒代測標本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項請與我司技術人員溝通。

3.液體類標本：標本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細胞培養上清、組織勻漿等。

4.購買前請提前告訴我們：您樣本的種屬、樣本數量、待測指標及其它特殊要求。

【雞神經膠質纖維酸性蛋白(GFAP)ELISA試劑盒廠家】五、代測數據詳細內容

1、ELISA標準曲線；

2、實驗具體詳細步驟；

3、完整的實驗報告（試劑耗材，實驗方法，實驗結果及結果說明）；

4、實驗進行階段，我公司客服人員會及時向您匯報實驗進程。

6000-5025        MST Conversion System            CNY                             

L2133       Expand 4 Pipettor Manual (CD-ROM)               CNY                             

9240708  Filter, Vacuum, 40 Micron, AP196/98                CNY                             

9243656  Post-validation Competency Training       """Post-validation Competency Lab Training includes training of the laboratory DNA staff on QIAGEN instrumentation ａnd chemistry, ａnd a review of information of the validation design plan ａnd results of the validation study. Training will be presented both via seminar ａnd hands-on-learning. Attendees will receive a Certificate of Attendance from QIAGEN to provide documentation of training. As required by FBI regulations, the laboratory is then responsible for the competency testing of all DNA analysts to perform lab work validated. The training presentation ａnd curriculum vitae of the trainer may be provided upon request.

Note:

The Post-validation Competency Training is required for all Comprehensive Validation Study plans but can be included at zero cost.

The Post-validation Competency Training is optional for both Guided ａnd Accelerated Validation Study plans ａnd so charges should apply accordingly.

"""   CNY                             

9022641  therascreen KRAS Assay Package CE                 CNY                             

5050-12200A   PC Country Kit, EU            CNY                             

274603     flocktype Mycoplasma Ms Ab (5)              CNY                             

285215     cador B. hyodysenteriae PCR Reagent (96)              CNY                              【雞神經膠質纖維酸性蛋白(GFAP)ELISA試劑盒廠家】

273405     pigtype Toxoplasma Ab (20)              CNY                             

273801     pigtype Yersinia Ab (1)               CNY                             

280375     virotype BVDV RT-PCR Kit (96)                  CNY                             

937156     QIAsymphony DSP AXpH DNA Kit (192)            CNY                             

9018702  EZ1 Adv. XL DSP DNA Blood Card              CNY                             

9001907  #QIAgility (w/o PC) PrioPLUS   "QIAgility (w/o PC)  Instrument

Installation of QIAgility instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

800337     Transformation                  CNY                             

9019616  ZLV Rail, worktable, plate, Cx2                  CNY                             

9019882  #Lid with LED, blue, RG6          CNY                             

9020585  Spring - Gas, Door Support, DML 3000             CNY                             

9011930  Reagent holder, tubes ａnd bottles  Holder for accommodating 4 microtubes (1.5 ml), 4 tubes (13 ml), ａnd 2 bottles (125 ml ａnd 60 ml) on the worktable   CNY                             

9013456  ZLV Buchsenleiste Autocom 2 pol AWG28                 CNY                             

9022131  Lid, careHPV             CNY                             

9022472  Shipment Box, DML3000                   CNY                             

9237133  #RapidPlate, Full Agreement  "Repair service delivery: 2 business days

Service response time (repair): Yes, 1/agreement year

Periodic inspection/maintenance: Onsite

Inspection/maintenance delivery: Yes, included

Cost coverage for repair parts: Yes, included

Cost coverage for labor: Yes, included

Cost coverage for travel: Yes, included

Replacement system (Loaner) provision: Not included

Transportation cost coverage: Not applicable"       CNY                             

9013394  Tubing set , HSPS, 200               CNY                              【雞神經膠質纖維酸性蛋白(GFAP)ELISA試劑盒廠家】

9013235  Tubing set,  90/120, vacuum, 0               CNY                             

9013226  Gripper set, L-handler, BR3000                 CNY                             

9013199  Tub.pre.sen.inlet,auto vac,BR8000 v1.00                 CNY                             

9011774  Tip Adapter 1100 ul BR8000             CNY                             

9011709  Dispenser head, BR8000          CNY                             

132559     NeXtal Stock Magnesium acetate (50)    1M Magnesium acetate tetrahydrate - 50mL (4 x 12,5 ml tube)    CNY                            

800309     siRNA Hairpin Run w/o prep             CNY                             

QY-x2254 COLO-320 人結腸腺癌細胞                                                

QY-x2255 COS-1 非洲綠猴SV40轉化的腎細胞                                                 

QY-x2256 COS-7 非洲綠猴SV40轉化的腎細胞                                                 

QY-x2257 CSSTH1 中華鱉心肌來源細胞                                                     

QY-x2258 CSSTL1 中華鱉肺來源細胞                                                  【雞神經膠質纖維酸性蛋白(GFAP)ELISA試劑盒廠家】

QY-x2259 CSSTSP1 中華鱉脾來源細胞                                               

QY-x2260 CWBRL 社鼠肺成纖維樣細胞                                                     

QY-x2261 CWbRS 刺毛鼠皮膚成纖維樣細胞                                                     

QY-x2262 Dami 人巨核細胞白血病細胞                                                    

QY-x2263 DCE 雞胚細胞                                                      

QY-x2264 DogL1 狗肺成纖維樣細胞                                                  

QY-x2265 DQSHS1 迪慶綿羊皮膚成纖維樣細胞                                               

QY-x2266 DtTRS1 褐腹鼠皮膚成纖維樣細胞                                                     

QY-x2267 DU145 人前列腺癌細胞                                                      

QY-x2268 E36，HGPRT-缺 中國倉鼠                                                  

QY-x2269 EJ 人膀胱癌細胞                                                 

QY-x2270 F81 貓腎細胞                                                       

QY-x2271 GHR2 金黃地鼠肋骨來源細胞                                                   

QY-x2272 GHS1 金黃地鼠皮膚成纖維細胞                                               

QY-x2273 GLC-15 人肺腺癌細胞                                                 

QY-x2274 GLC-82 人肺腺癌細胞                                                  【雞神經膠質纖維酸性蛋白(GFAP)ELISA試劑盒廠家】

QY-x2275 GPH4 豚鼠心肌來源細胞                                                   

QY-x2276 GPL1 豚鼠肺成纖維樣細胞                                                

QY-x2277 GPS5 豚鼠皮膚成纖維樣細胞                                                    

QY-x2278 H2EL 人胚胎成纖維細胞                                                    

QY-x2279 H-4-II-E 大鼠肝細胞瘤細胞                                                

QY-x2280 H9 人急性淋巴母細胞白血病細胞                                                    

QY-x2281 HAC-84 人肺泡癌細胞                                                

QY-x2282 Hacat 人永生化角質細胞                                                   

QY-x2283 HAL-01 人急性混合型白血病細胞