1. 酶標儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【友情提示】：本產品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！【雞血小板因子4(PF-4/CXCL4)ELISA試劑盒現貨】

ELISA應用：

ELISA檢測試劑盒、ELISAKit、酶聯免疫試劑盒、酶聯免疫吸附測定試劑盒、酶聯免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認可及推崇,在歐美及中國獲得很大的推廣,尤其是國內生化領域的長足發展.Elisa生物試驗是一種敏感性高,特異性強,重復性好的實驗診斷方法.由于其試劑穩定、易保存,操作簡便,結果判斷較客觀等因素,已廣泛應用在免疫學檢驗的各領域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【雞血小板因子4(PF-4/CXCL4)ELISA試劑盒現貨】齊一生物銷售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【雞血小板因子4(PF-4/CXCL4)ELISA試劑盒現貨】ELISA實驗原理

試劑盒是固相夾心法酶聯免疫吸附實驗（ELISA）.已知濃度的標準品、未知濃度的樣品加入微孔酶標板內進行檢測.先將生物素標記的抗體同時溫育.洗滌后,加入親和素標記過的HRP.再經過溫育和洗滌,去除未結合的酶結合物,然后加入底物A、B,和酶結合物同時作用.產生顏色.顏色的深淺和樣品中的濃度呈比例關系.

免疫測定技術的基礎在于抗原抗體之間的特異結合反應,所以任何優質的診斷試劑離不開優質的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術得到的單克隆抗體,而抗原或抗體的標記物如酶標結合物則通過各種化學合成方法制備.近年來,隨著分子生物學的發展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結合物等免疫測定試劑幾成為現實的新一代試劑,各種新型使用的測定方法也不斷出現.

HBsAg試劑特點（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應性.酶表抗體為與HBsAg有不同結合位點的鼠復合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應用ParlEhrich學說（PEI）HBsAg標準品,對ad標準品的靈敏度為0.05ng/ml對ay標準品靈敏度為0.025ng/ml

ELISA檢測試劑盒應用定性夾心免疫檢測技術,用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標準品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結合物,經第二次孵育后,酶結合物就會與次孵育結合上的HEVIgM抗體相結合,洗板除去未結合的酶結合物,加入TMB底物溶液,在第三次孵育時會發生酶-底物反應,只有那些含有HEVIgM抗體和酶結合物所形成的復合物的孔才會發生顏色變化,加入硫酸溶液終止酶和底物間的反應,并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標準,O.D.值大于或等于Cut-Off值的樣品被認為是初試陽性.

以上資料僅供參考了解，不供于論文寫作

【雞血小板因子4(PF-4/CXCL4)ELISA試劑盒現貨】齊一生物專業為您提供高品質，高靈敏度.高質量的酶聯免疫試劑盒，齊一生物長期經營ELISA試劑盒（免費代測）及抗體、分子學生物試劑盒、金標法檢測試劑盒、比色法檢測試劑盒、細胞菌株、培養基、細胞因子、生化試劑、耗材等生物試劑產品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

9238645 Brake resistor 112 Ohm / 100 W      CNY            

9242061 Pipetting container, IQ/OQ, QS      CNY            

9241827 Pyro Q96 ID, Premium Agreement  "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"   CNY            

9241166 Board, A/D, QX      CNY            

9240955 Sensor, z, handler, Qsym        CNY            

9240835 QIAcube, Basic Agreement, no PM The Basic Agreement QIAcube provides full service coverage for the QIAcube. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Agreement, there is a priority response time of 5  working days.   CNY            

9240766 32 OUT Module, D2-32TD1, AP96       CNY            

6000-5018   MST STM Rack & Lid (Blue - 2 pos. cal.)     CNY            

9241777 QIAxtractor, Inspection Service "The Annual Inspection Service, QIAxtractor is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAextractor are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report."    CNY            

9241375 Magnet, door lock, top, EZ1a        CNY            

9241334 Keypad foil, EZ1        CNY            

9240750 Belt, Elevator, AP      CNY            

9243648 Acceler. Valid Supp, Other Extract Sys  The QIAGEN Accelerated Validation Support Other Extraction System service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide provide an introduction to the instrument ａnd hands-on user support.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Attendees will receive a certificate of participation in the hands-on exercises.  Please indicate which protocols to validate (Biosprint 96, DNA mericon).    CNY            

9243553 #BR M96, PM Service "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"   CNY             【雞血小板因子4(PF-4/CXCL4)ELISA試劑盒現貨】

9242864 QIArent&go -Rent out fee QSSP/AS Cabinet        CNY            

5000-1321   Feet Set/4, SKR1        CNY            

5000-1288   DML 2000 - Fuse 160 mA PKG 10       CNY            

5000-1287   DML 2000 - Fuse 315 mA PKG 10       CNY            

5000-1505   Switch ON-OFF 120V, HTR1        CNY            

6000-00181  Plate Washer - Replacement Tubing Kit       CNY            

6000-00178  Plate Washer - Maintenance Kit      CNY            

5100-00047  Wash Buffer Concentrate (2 Bottles) "2 bottles of 100mL each.

The price is proportional to 2x 5100-0795 (discontinued)"   CNY            

9240740 Tubing, Tygon, 1/4" ID      CNY            

9240735 Tubing, Black, Capper, AP196/98     CNY            

9240724 Tube Assy, Draw, AP96/196       CNY            

5000-1412   Poteneiometer 500K, VTX2        CNY            

9240506 Monitor, 17", Flat Panel, AP        CNY            

9239851 Cover, LC200        CNY            

9239565 xMAP Alignment Microspheres     CNY            

9019942 Barrel removal tool, CX1        CNY             【雞血小板因子4(PF-4/CXCL4)ELISA試劑盒現貨】

9019474 Lid, UV, polycarb, C1x      CNY            

QY-x1954    COLO 205 結腸癌 1640+10%胎牛血清                        【雞血小板因子4(PF-4/CXCL4)ELISA試劑盒現貨】

QY-x1955    COLO320DM 結腸癌 1640+10%胎牛血清                      

QY-x1956    HCT-8 回盲腸腺癌 1640+10%胎牛血清                      

QY-x1957    HCT-116 低分化結腸腺癌 1640+10%胎牛血清                    

QY-x1958    HT-29 結腸腺癌 1640+10%胎牛血清                    

QY-x1959    LS-174T 結腸腺癌 MEM+非必需氨基酸+10%胎牛血清                      

QY-x1960    SW480 結腸癌 L15                       

QY-x1961    BEL-7402 肝細胞癌 1640+10%胎牛血清                     

QY-x1962    BEL-7405 肝細胞癌 1640+10%胎牛血清                     

QY-x1963    H7402 肝癌 1640+10%胎牛血清                    

QY-x1964    HCC-9204 肝癌 1640+10%胎牛血清                     

QY-x1965    Hep3B 肝癌 DMEM+非必需氨基酸+10%胎牛血清                       

QY-x1966    Hep G2 肝癌 DMEM+10%胎牛血清                       

QY-x1967    SMMC-7721 肝癌 1640+10%胎牛血清                    

QY-x1968    PANC-1 胰腺導管上皮癌 1640+10%胎牛血清                     

QY-x1969    Panc 10.05 胰腺腺癌 高糖1640+15%胎牛血清+胰島素等                      

QY-x1970    SW1990 胰腺腺癌 1640+10%胎牛血清                        【雞血小板因子4(PF-4/CXCL4)ELISA試劑盒現貨】

QY-x1971    JF305 胰腺癌 1640+10%胎牛血清                      

QY-x1972    PC-3 胰腺癌 1640+10%胎牛血清                       

QY-x1973    Capan-2 胰腺癌 1640+10%胎牛血清                    

QY-x1974    SK-RC-42 腎癌 1640+10%胎牛血清                     

QY-x1975    95-C 低轉移肺癌 1640+10%胎牛血清                       

QY-x1976    95-D 高轉移肺癌 1640+10%胎牛血清                       

QY-x1977    973 肺腺癌 1640+10%胎牛血清                    

QY-x1978    A2 肺腺癌 1640+10%胎牛血清                     

QY-x1979    A549 肺腺癌 1640+10%胎牛血清                       

QY-x1980    A549/DDP 肺腺癌/順鉑耐藥株 1640+10%胎牛血清                    

QY-x1981    Calu-3 肺腺癌 MEM+非必需氨基酸+10%胎牛血清                     

QY-x1982    GLC-82 肺腺癌 1640+10%胎牛血清 已有                    

QY-x1983    H1299 非小細胞肺腺癌 1640+10%胎牛血清