ELISA酶聯法試劑盒操作因固相載體的形成不同而有所差異，國內醫學檢驗一般均用板式點。本文將敘述板式ELISA各個操作步驟的注意要點，珠式、管式及磁性球ELSIA，國外試劑均與特殊儀器配合應用，兩者均有詳細的使用說明，嚴格遵照規定操作，必能得出準確的結果。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

首先齊一生物優質的試劑盒，良好的儀器和正確的操作是保證ELISA檢測結果準確可靠必要條件。所以ELISA試劑盒的購買，試劑質量的保證以及實驗前實驗儀器的檢察都是很有重要性的。保證實驗材料的質量之后要嚴格按照實驗步驟進行。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【雞促卵泡素(FSH)ELISA試劑盒現貨】齊一生物ELISA試劑盒

1.精心挑選的進口抗體、標準品、顯色劑和酶標板全部為進口產品實現*佳性能；

2.原材料批量進口加上自配的特殊配方稀釋劑保證高品質產品性能的同時兼顧成本；

3.采用技術，嚴格按照標準生產方案組裝生產；

4.預包被微孔板的批內和批間變異系數均小于10 ％；

5.經過嚴格交叉反應和干擾測試及穩定性試驗；

6.明確的敏感度；

7.針對所有樣品類型達到*佳的性能；

8.廣泛的檢測范圍可以滿足大多數檢測要求；

9.完善的售后服務和免費的技術支持免去您的后顧之憂；

10.強大的生產能力和充足的儲備、操作熟練的技術人員及高效的生產流程，隨時可以組裝出您定制的產品。

【雞促卵泡素(FSH)ELISA試劑盒現貨】齊一生物僅供實驗人、大鼠、小鼠、兔等動物實驗標本檢測使用！得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！齊一生物銷售：021－6034 8496；181214 53965；173021 04490

ELISA試劑盒問題描述

標準曲線梯度差：

吸液或加液不準請檢查移液器及吸頭

標準品稀釋不正確溶解標準品稍微旋轉瓶身，輕輕混勻使粉末完全溶解

洗滌不完全保證洗滌時間和洗滌次數及每孔的加液量

顯色很弱或無色：

孵育時間太短保證充足的孵育時間

實驗溫度不正確使用推薦的實驗溫度

試劑體積不夠或漏加、稀釋不正確檢查吸液及加液過程，保證所有試劑按順序足量添加

讀數數值低：

酶標儀設置不正確 在酶標儀上檢查波長及濾光片設置

提前打開酶標儀預熱：變異系數大加液不正確檢查加液情況

【雞促卵泡素(FSH)ELISA試劑盒現貨】背景值高：

檢測抗體的工作濃度使用推薦的稀釋倍數

酶標板洗滌不完全重新閱讀操作手冊，保證清洗完全；如果用自動洗板機，請檢查所有的出口是否有堵塞

洗液有污染配制新鮮的洗液

靈敏度低：

ELISA試劑盒保存按說明書要求保存相關試劑

讀數前未終止OD讀數前應在每孔中加入終止液

齊一生物客戶寄標本時需注明以下情況：

1、標本編號；2、所測項目；3、是否做復孔；3、聯系方式；4、實驗后標本是否寄回。

客戶必知：

客戶應對所提供的材料及信息負責，如因客戶提供的材料及信息不準確而引起的實驗延誤或經濟損失由客戶承擔。

試劑盒樣本要求

在收集標本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩定。我們提倡新鮮標本盡早檢測，對收集后當天就進行檢測的標本，及時儲存在4℃備用，如有特殊原因需要周期收集標本，請造模取材后，將標本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質量，所以避免反復凍融。具體操作注意事項請與我司技術人員溝通。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【雞促卵泡素(FSH)ELISA試劑盒現貨】液體類標本：標本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細胞培養上清、組織勻漿等。

1. 血清：室溫血液自然凝固10-20分鐘后，離心20分鐘左右（2000-3000轉/分）。仔細收集上清。保存過程中如有沉淀形成，應再次離心。

2. 血漿：應根據標本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑，混合10-20分鐘后，離心20分鐘左右（2000-3000轉/分）。仔細收集上清。保存過程中如有沉淀形成應再次離心。

3. 尿液、胸腹水、腦脊液：用無菌管收集。離心20分鐘左右（2000-3000轉/分）。仔細收集上清。如有沉淀形成，應再次離心。

4. 細胞培養上清：檢測分泌性的成份時，用無菌管收集。離心20分鐘左右（2000-3000轉/分）。仔細收集上清。

5. 培養細胞:檢測細胞內的成份時，用PBS（PH7.2-7.4）稀釋細胞懸液，細胞濃度達到100萬/ml左右。通過反復凍融或加入組織蛋白萃取試劑，以使細胞破壞并放出細胞內成份。離心20分鐘左右（2000-3000轉/分）。仔細收集上清。保存過程中如有沉淀形成，應再次離心。

6. 組織標本：切割標本后，稱取重量。加入一定量的PBS，緩沖液中可加入1μg/L蛋白酶抑制劑或50U/ml的Aprotinin（抑肽酶）。用手工或勻漿器將標本勻漿充分。離心20分鐘左右（2000-3000轉/分）。仔細收集上清置于-20度或-70度保存，如有必要，可以將樣品濃縮干燥。分裝后一份待檢測，其余冷凍備用。

7. 標本采集后盡早進行提取，提取按相關文獻進行，提取后應盡快進行實驗。若不能馬上進行試驗，可將標本放于-20℃保存，但應避免反復凍融

8.不能檢測含NaN3的樣品，因NaN3抑制辣根過氧化物酶的（HRP）活性。

9.如果樣品不能立即檢測，應將其分裝，-70 ℃保存，避免反復冷凍。保存過程中如出現沉淀，應再次離心。血液標本盡可能的不要使用溶血或高血脂血。如果血清中含大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解

凍。應在室溫下解凍并確保樣品均勻地充分解凍。

9240769  Mono Touch Screen, AP96           CNY                           

9241465  IQ/OQ Services, QIAcube     Instrument qualification support service for the QIAcube. The product contains the IQ/OQ documentation ａnd the performance of the qualification protocols (travel ａnd labor costs included).       CNY                           

9243647  Acceler. Valid Supp, QuantiPlex/QP Hyres    The QIAGEN Quantiplex/Quantiplex HYres Accelerated Validation Support service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introductory seminar about the Quantiplex/Quantiplex HYres quantification chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on user reaction setup, ａnd best practices.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through Quantiplex/Quantiplex HYres validation, as well as general wet lab support such as pipetting for quantification to produce the data needed for the validation study. The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support.  CNY                           

9243646  Guided Valid Support, Other Quant. Sys       The QIAGEN Guided Validation Support, Other Quantification System service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide hands-on user reaction setup, ａnd best practices.  The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which protocols to validate (Plexor HY, Quantifiler Human, Quantifiler Y, Quantifiler Duo)  CNY                           

9243541  #Rapid Plate, PM Service      "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage" CNY                           

9243115  Lee Sample Valve, LC           CNY                           

9242863  QIArent&go - Rent out fee QSAS Cabinet           CNY                           

9242771  Tool, align encoder, Qsym            CNY                           

9242717  Validation Support, 5d, QIAcube   The QIAGEN QIAcube Accelerated Validation Support service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the QIAcube system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on user support ａnd QIAcube instrument operation. The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Please indicate which protocols to include in the validation (QIAamp, QIAamp DNA Investigator, MinElute PCR Cleanup, etc.).       CNY                           

9242204  Adapter, 2x Rotor-Disc, Qsym             CNY                            【雞促卵泡素(FSH)ELISA試劑盒現貨】

5000-1020R    DML 2000 Luminometer, 240V (Refurbished)            CNY                           

5000-1323      Rotary Shaker - Cord Line, 220V         CNY                           

5000-1317      Knob Speed Timer, SKR1             CNY                           

5000-1272      X Drive Track, DML2          CNY                           

5000-1260      Stepper Model New Styl, DML2          CNY                           

5001-2240      Hybrid Capture 2 (hc2)System Core, 240V         CNY                           

5000-1506      Switch ON-OFF 240V, HTR1              CNY                           

6000-4111ENV2.2  RCS User Application Manual (English)        CNY                           

6000-3117      Plate Washer I - Maintenance Kit          CNY                           

5199-1220      hc2 High-Risk HPV DNA Test             CNY                           

5126-1220      hc2 DNA Collection Device          CNY                           

GR8RNA QIAshredder (250)/RNeasy (250) spezial            CNY                           

RNEZ4U QIAshredder (50)/RNeasy (50) special        CNY                           

9240736  Decon tube, Actril, AP98              CNY                           

5000-1410      Strip Shock Absorbing, VTX2             CNY                           

9240692  Fan, 12Vdc, PC-104 cover, AP196/98         CNY                            【雞促卵泡素(FSH)ELISA試劑盒現貨】

51331     QIAamp 96 DNA QIAcube HT Kit (5)         CNY                           

74171     RNeasy 96 QIAcube HT Kit (5)           CNY                           

180994    GeneRead Adapter L Set 12-plex (72)          CNY                           

9002140  careHPV test Luminometer           CNY                           

9230355  Washstation P1xxx        CNY                           

QY-x1354       人舌癌細胞系Tca8113-P160                                         

QY-x1355       人舌癌細胞T6                                        

QY-x1356       人舌鱗癌細胞系TSCCA                                        

QY-x1357       上頜牙齦癌頸淋巴結轉移癌GNM                                       

QY-x1358       人星形膠質瘤細胞U251                                        

QY-x1359       人退行性癌細胞Calu-6                                   

QY-x1360       人羊膜細胞HA                                       

QY-x1361       人羊膜細胞WISH                                         

QY-x1362       人胚纖維細胞系HEF                                       【雞促卵泡素(FSH)ELISA試劑盒現貨】

QY-x1363       人皮膚成纖維細胞CCC-HSF-1                                     

QY-x1364       逆轉錄病毒包裝用的人胚胎成纖維細胞PA317                                   

QY-x1365       人髓母細胞瘤細胞D341Med                                        

QY-x1366       人APP-PS1(C410Y)雙基因轉染細胞株7WCY1.0                                       

QY-x1367       人APP基因轉染CHO細胞株7WD10                                         

QY-x1368       人APP-PS1(M146L)雙基因轉染CHO細胞株7WML6.0                                    

QY-x1369       人APP-PS1雙基因轉染CHO細胞株7WPS1                                      

QY-x1370       人干細胞因子單克隆抗體細胞株AMS2                                      

QY-x1371       人APP-PS1雙基因轉染細胞株(HEK293)APP-PS1                                     

QY-x1372       人胚胎眼鞏膜成纖維細胞HFSF                                    

QY-x1373       人胚胎眼Tenon＇s囊成纖維細胞HFTF                                      

QY-x1374       小鼠白血病細胞M1                                       

QY-x1375       小鼠白血病細胞L1210                                   

QY-x1376       小鼠白血病細胞P388                                     

QY-x1377       小鼠單核巨噬細胞白血病細胞RAW 264.7                                         

QY-x1378       大鼠嗜堿性細胞白血病細胞RBL-2H3                                          【雞促卵泡素(FSH)ELISA試劑盒現貨】

QY-x1379       小鼠淋巴樣瘤細胞P388D1                                    

QY-x1380       小鼠白血病克隆細胞系L6565                                      

QY-x1381       小鼠白血病細胞G-CSF依賴性NFS-60                                       

QY-x1382       小鼠肥大細胞癌細胞P815                                     

QY-x1383       小鼠肺癌細胞Lewis                                       

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9020101  Shipping Lock Washer, TLyser 2                 CNY                             

9239503  Check Valve, LC                  CNY                             

9239489  EX RTD Adapter bd. XYP           CNY                             

9239483  EX Syringe Pump LX100             CNY                             

9232835  Tips Disposable Eppendorf 1.1 ml             CNY                             

9232804  Filter, Interference, 550nm              CNY                             

9239363  Inlet-micro-fuse T 3.15A, 250V, EZ1                  CNY                             

9239343  MDx Protocol Installation, on-site, 2day          CNY                             

9239263  BioSprint Software            CNY                              【雞白血病病毒抗原(ALV-AG)ELISA試劑盒廠家】

9236440  0.5 hr                 CNY                             

9239156  Assembly, gripper, TWI             CNY                             

9239152  Screw, support fixation (to base), TWII             CNY                             

9239006  Z-Rack Packing Cover                CNY                             

9232667  Power supply, peristaltic pump, 1.02                CNY                             

9232630  Bottle, 250-ml, white luer adapter          CNY                             

9232618  Socket with Switch/Fuse           CNY                             

9232603  Vacuum manifold, strip holder bottomless                CNY                             

9232561  Tubing Connector di=6mm, M10x1          CNY                             

9238965  Fastening element for pneumatic spring                   CNY                             

9238960  Serial Cable, F9/F25, BS            CNY                             

9016903  Upgrade kit, needle, w-drive, BR8           CNY                             

9016857  XYP IS V2.3                CNY                             

9232557  Vacuum manifold, plate holder 1.02                 CNY                             

9238941  PCB, CSU-01, BS15            CNY                             

9241121  Loctite 425                CNY                              【雞白血病病毒抗原(ALV-AG)ELISA試劑盒廠家】

9241120  Loctite 242                CNY                             

9240957  PCB, gripper, handler, Qsym             CNY                             

9240953  Motor, z,  handler, Qsym                 CNY                             

9240781  500 ml Reagent #6 Update, AP96            CNY                             

9240780  500 ml Reagent #6 Update, AP196          CNY                             

9240772  Assy, Sub, Dispense Head, AP96                CNY                             

QY-x1364 人黑色素瘤細胞SK37                                                  

QY-x1365 人黑色素瘤細胞A875                                                 

QY-x1366 人皮膚黑色素瘤細胞SK-MEL-1                                                  

QY-x1367 人惡性黑色素瘤細胞A375                                                

QY-x1368 人侵襲性脈絡膜黑色素瘤細胞M619                                                

QY-x1369 人黑色素瘤細胞 M14                                               

QY-x1370 人葡萄膜黑色素細胞UM                                                     【雞白血病病毒抗原(ALV-AG)ELISA試劑盒廠家】

QY-x1371 人視網膜色素上皮細胞D407                                                     

QY-x1372 人視網膜母細胞瘤Rb                                                 

QY-x1373 人眼脈絡黑色素瘤細胞OCM-1                                                  

QY-x1374 人眼脈絡黑色素瘤細胞MuM－2C                                                     

QY-x1375 人眼脈絡黑色素瘤細胞MuM－2B                                                     

QY-x1376 人低侵襲性脈絡膜黑色素素瘤細胞OCM-1A                                                    

QY-x1377 人視網膜母細胞瘤Y79                                               

QY-x1378 熒光素酶轉染人成骨肉瘤細胞U2OS-Luc teT-on                                              

QY-x1379 人橫紋肌肉瘤細胞TE671 subline No.2                                              

QY-x1340 人眼脈絡黑色素瘤細胞C918                                                     

QY-x1341 人橫紋癌細胞A673                                                      

QY-x1342 人惡性胚胎橫紋肌瘤細胞RD                                                     

QY-x1343 人表皮癌細胞A-431                                                    

QY-x1344 人正常皮膚細胞 HaCaT                                                      

QY-x1345 人皮膚纖維微細胞系Malmc                                                       

QY-x1346 人皮膚成纖維細胞系BJ                                                        【雞白血病病毒抗原(ALV-AG)ELISA試劑盒廠家】

QY-x1347 人成纖維肉瘤細胞HT1080                                               

QY-x1348 人皮膚癌細胞系HS-1                                                  

QY-x1349 人皮膚癌細胞系HS-4                                                  

QY-x1350 人胸膜瘤細胞SMC-1                                                   

QY-x1351 人胸腺激酶缺陷性細胞系 143TK                                                       

QY-x1352 人舌癌細胞Tca-8113                                                   

QY-x1353 人舌癌細胞系Tca8113-P60