檢測標本：血清,血漿,尿液,胸腹水,腦脊液,細胞培養上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯免疫夾心法

產品的用途：僅供科研究課題使用

價格及詳細資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認同.大品牌保證,價格公道,傾力為國內外科研院校實驗室提供*優質的產品.若有需要,我司將竭誠為您服務！本試劑盒用于測定血清,血漿及相關液體樣本中含量.

【雞硫酸類肝素(HS)ELISA試劑盒廠家】實驗原理：

   本試劑盒應用雙抗體夾心法測定標本中該產品水平.用純化的本產品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產品抗原,再與HRP標記的本產品抗體結合,形成抗體-抗原-酶標抗體復合物,經過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉化成藍色,并在酸的作用下轉化成*終的黃色.顏色的深淺和樣品中的本產品呈正相關.用酶標儀在450nm波長下測定吸光度（OD值）,通過標準曲線計算樣品中該產品濃度.

試劑盒組成：

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【雞硫酸類肝素(HS)ELISA試劑盒廠家】標本要求：

1．標本采集后盡早進行提取,提取按相關文獻進行,提取后應盡快進行實驗.若不能馬上進行試驗,可將標本放于-20℃保存,但應避免反復凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標準品的稀釋與加樣：在酶標包被板上設標準品孔10孔,在、第二孔中分別加標準品100μl,然后在、第二孔中加標準品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標準品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標準品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標準品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標準品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【雞硫酸類肝素(HS)ELISA試劑盒廠家】

2.     加樣：分別設空白孔（空白對照孔不加樣品及酶標試劑,其余各步操作相同）、待測樣品孔.在酶標包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標板孔底部,盡量不觸及孔壁,輕輕晃動混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復5次,拍干.

6.     加酶：每孔加入酶標試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應（此時藍色立轉黃色）.

11. 測定：以空白空調零,450nm波長依序測量各孔的吸光度（OD值）. 測定應在加終止液后15分鐘以內進行.

注意事項：

1． 試劑盒從冷藏環境中取出應在室溫平衡15-30分鐘后方可使用,酶標包被板開封后如未用完,板條應裝入密封袋中保存.

2． 濃洗滌液可能會有結晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結果. 【雞硫酸類肝素(HS)ELISA試劑盒廠家】

3． 各步加樣均應使用加樣器,并經常校對其準確性,以避免試驗誤差.一次加樣時間控制在5分鐘內,如標本數量多,推薦使用排搶加樣.

4． 請每次測定的同時做標準曲線,做復孔.如標本中待測物質含量過高（樣本OD值大于標準品孔孔的OD值）,請先用樣品稀釋液稀釋一定倍數（n倍）后再測定,計算時請*后乘以總稀釋倍數（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請避光保存.

7． 嚴格按照說明書的操作進行,試驗結果判定必須以酶標儀讀數為準.

8． 所有樣品,洗滌液和各種廢棄物都應按傳染物處理.

9． 本試劑不同批號組分不得混用.

10. 如與英文說明書有異,以英文說明書為準.

計算：

以標準物的濃度為橫坐標,OD值為縱坐標,   

在坐標紙上繪出標準曲線,根據樣品的OD     

值由標準曲線查出相應的濃度；再乘以稀釋     

倍數；或用標準物的濃度與OD值計算出標     

準曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計算出樣品濃度,再乘以稀釋

倍數,即為樣品的實際濃度.

試劑盒性能：

1.樣品線性回歸與預期濃度相關系數R值為0.95以上.

2.批內與批見應分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個月

6000-5003        Duraseal Sealing Film                CNY                             

9241781  RGQ, Full Agreement, no PM  "Repair service delivery: send to Regional Repair Center

Service response time (repair): 7-10 days turnaround time

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: yes, included (provision time 2 days)

Transportation cost coverage: yes, included" CNY                             

9241776  QIAgility, Inspection Service    "The Annual Inspection Service, QIAgility is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAgility are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report."    CNY                             

9241756  IQ/OQ Services, QIAsymphony         Instrument qualification support service for the QIAsymphony. The product contains the Installation Qualification (IQ)/Operation Qualification (OQ) documentation ａnd the performance of the qualification protocols (travel ａnd labor costs included).         CNY                             

9237126  Upgrade App., PCR Setup                  CNY                             

9237093  Training, Extension day    Additional days of training may be purchased during the week of the site visit upon request.         CNY                              【雞硫酸類肝素(HS)ELISA試劑盒廠家】

9236426  Board, arm interconnect, TWI                  CNY                             

9236421  Switch, firmware loading, TWI                  CNY                             

9236359  Sheath Filter with Quick Disconnect                  CNY                             

9236346  1.5A Fuse LiquiChip           CNY                             

9236338  Quick disconnect Set                  CNY                             

9236164  Syringe block, repl. kit, RapidPlate           CNY                             

9236153  Locator Plate, Tips, pos 2, BR RP              CNY                             

9235698  Cable 24V, RCS1                 CNY                             

9235296  Capillary, Dispenser head, Mounting Tool                 CNY                             

9235054  Incubator Tower (65°C) Board                   CNY                             

9234941  Incubator Tower (65°C) Board DINCS                CNY                             

9238658  Cooling compressor T2178GK, 50Hz                  CNY                             

9242060  Adapter plate, IQ/OQ, QS                 CNY                             

9242026  Repair Thermo, Depot Repair, Palm Cycler              CNY                             

9241824  Pyro Q96 MD, Full Agreement         The Full Agreement, PyM provides full service coverage for the PyroMark instrument. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one on-site inspection service, with costs for labor ａnd parts covered. With a Full Agreement, there is a response time of 2 working days.         CNY                             

9241815  Pyro Q96 ID, PM Agreement            CNY                              【雞硫酸類肝素(HS)ELISA試劑盒廠家】

9241172  anti-rotation tool              CNY                             

9240960  Cable, cLLD, pipetting system, Qsym                CNY                             

9240949  Cable, y, portal, Qsym               CNY                             

9240678  Bimba Rotary Actuator, AP               CNY                             

9240777  CE Update, AP196/98                 CNY                             

9241773  RGQ, Installation     The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the Rotorgene-Q systems hardware ａnd trains the user on the operation of the Rotorgene software. After the training the user will be able to develop application programs, conduct a basic troubleshooting, ａnd understand the application output format.All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements.     CNY                             

9241763  QIAgility, Full Agreement         "Repair service delivery: onsite

Service response time (repair): 48 hours/2 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) or PC repair provision: not included

Transportation cost coverage: not applicable"        CNY                             

9241372  Reflector, UV lamp, EZ1a                  CNY                             

9243545  QIAsymphony SP, PM Service   "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

QY-x1274 人T淋巴瘤轉基因細胞Jurkat D,E                                                       【雞硫酸類肝素(HS)ELISA試劑盒廠家】

QY-x1275 人T淋巴瘤細胞Jurkat亞系Jurkat77                                                

QY-x1276 人外周血嗜堿性白細胞KU812                                                   

QY-x1277 人原髓細胞白血病細胞AL7P/HL-60R                                                

QY-x1278 人急性成髓細胞白血病Kasumi-1                                              

QY-x1279 人T細胞白血病細胞T-CEM                                               

QY-x1280 人EB病毒轉化的B細胞CGM1                                                 

QY-x1281 人血液白血病細胞TF-1                                                       

QY-x1282 人分泌A2抗體B淋巴細胞BB7.2                                                      

QY-x1283 人血管平滑肌 T/G                                                       

QY-x1284 急性T淋巴細胞白血病細胞TALL-104                                               

QY-x1285 人淋巴細胞瘤細胞JK(Jurkat)                                                       

QY-x1286 人T淋巴瘤細胞系Hut-102                                                

QY-x1287 人Burkitt`s淋巴瘤細胞Raji                                                

QY-x1288 人B細胞淋巴瘤細胞系BJAB                                                      

QY-x1289 人Burkitts淋巴瘤細胞系CA46                                                   

QY-x1290 血細胞瘤細胞系Ramos                                                        【雞硫酸類肝素(HS)ELISA試劑盒廠家】

QY-x1291 人B淋巴細胞瘤細胞RAMOS(RA.1)                                                   

QY-x1292 人Burkitt`s淋巴瘤細胞 NAMALWA                                                    

QY-x1293 分泌A2抗體B淋巴細胞BB7.2                                               

QY-x1294 轉HLA-2基因B細胞T2                                                       

QY-x1295 人EBV陽性B淋巴瘤細胞P3HR1                                                       

QY-x1296 人組織細胞淋巴瘤細胞U937                                                     

QY-x1297 人急性T淋巴母細胞白血病細胞MOLT-4                                                 

QY-x1298 人T細胞淋巴瘤Hat-78                                                       

QY-x1299 人T細胞淋巴瘤Hurt-78                                                      

QY-x1300 人T淋巴細胞系H9                                                      

QY-x1301 人T淋巴細胞白血病,CD8+Molt-4                                                       

QY-x1302 人B淋巴母細胞HMy2.CIR                                                 

QY-x1303 人急性淋巴細胞白血病T淋巴細胞CCRF-CEM