【虹膜色素上皮細胞總DNA】下面是細胞株培養的具體無菌技術要點：

1. 實驗開始前，無菌室和無菌操作臺需要紫外光照射30到60分鐘完全滅菌，用70%ethanol擦拭無菌操作臺面，并且打開無菌操作臺風扇運行10分鐘之后，才可以進行實驗操作。每次操作只能處理一株細胞株，即使培養基完全相同也不可共用培養基，以避免細胞間污染或失誤混淆。實驗完成后，請將實驗物品拿出工作臺，用70%ethanol再次擦拭無菌操作臺面。操作間隔應該讓無菌操作臺運轉10分鐘到15分鐘后，才能進行下一個細胞株的操作。

2. 無菌操作工作的區域應保持清潔和寬敞，必要物品（試管架、吸管、吸取器或吸管盒等）可以暫時放置，其它實驗用品使用完畢后應移出，有利于空氣流通。實驗用品需70%ethanol擦拭后方可帶入無菌操作臺內。實驗操作過程應在臺面的中央無菌區域內完成，請勿在邊緣非無菌區域進行操作。

3. 小心取用無菌實驗物品，避免細菌污染。請勿觸碰吸管尖頭部和容器瓶口處，也不要在打開的容器正上方進行操作。容器打開后，請用手夾住瓶蓋并輕握瓶身，傾斜大約45°角取用，盡量不要將瓶蓋口朝上放置于桌面。

4. 工作人員應當首先注意自身安全，必須穿戴齊實驗衣和實驗手套后才能進行實驗。對于病毒感染或是來自人類的細胞株應當特別小心操作，并選擇適當等級的無菌操作臺進行（至少是Class II）。操作過程中，應避免引起aerosol的產生，小心有毒性藥品，例如DMSO和TPA等，并避免針頭的傷害等等。

5. 定期檢測下列項目：

5.1. CO2鋼瓶的CO2壓力

5.2. CO2培養箱的CO2濃度、溫度、和水盤是否有污染（水盤的水需用無菌水，每周定時更換）。

5.3. 無菌操作臺內的airflow壓力，定期更換紫外線燈管和HEPA過濾膜，預濾網（300小時/預濾網，3000小時/HEPA）。

6. 水槽內可添加消毒劑（Zephrin 1:750），需定期更換水槽中的水。

【虹膜色素上皮細胞總DNA】齊一生物友情提醒，本產品僅供科研使用，不得用于臨床及診斷使用！

一細胞說明，具體詳細請見細胞附帶說明書

 二、客戶自備試劑

1、PBS  

2、 2、RPMI-1640 培養基（不含 Hepes）+ 10%胎牛血清+ 1%雙抗

3、0.25%  （W/V）胰蛋白酶（含 0.02% EDTA）

三、細胞背景

1、生長方式：貼壁

2、種屬：Homo sapiens  人

四、培養條件

1、培養基：RPMI-1640 培養基（不含 Hepes）+ 10%胎牛血清+ 1%雙抗

2、溫度：37.0°C

3、氣體:空氣  95%，CO2 5%

【虹膜色素上皮細胞總DNA】五、培養方法

 收到細胞后，在倒置顯微鏡下觀察整個細胞生長情況：

 如果細胞未長滿，用 75%酒精噴灑整個瓶消毒后放到超菌臺內，嚴格無菌操作，打開細胞培養瓶，留10ml 培養液繼續培養。如果細胞已長滿（達 80-90%）。即可進行傳代，具六、體步驟如下：

1，棄去培養液，用PBS洗 1-2次。

2，向瓶內加入 1.0-2.0ml 胰蛋白酶液，在倒置顯微鏡下觀察細胞消化情況，若細胞大部分變圓，迅速拿回操作臺，吸取胰蛋白酶，加含有 6ml  含 10%血清的培養液，輕輕吹打細胞。

3，加入等量的的培養液，輕輕吹打混勻后吸出一半，分到新的培養

4，傳代比例：1:2-1:3  

七、常見問題及解決方案

1、培養瓶有破裂，培養液有漏液：細胞極大可能會污染，所以我們會及時安排幫老師解決。

2、細胞漂�。号囵B瓶不開封，瓶口酒精擦拭后平躺放置在培養箱。次日觀察，如細胞大部分又貼回瓶底，表明細胞活力正常，剩余漂浮的細胞可以去掉，留 10ml 培養液培養觀察，細胞生長至匯合度 80%，進行消化傳代；如細胞還是不貼壁，將細胞離心收集轉到新培養瓶，原培養瓶加部分培養液繼續培養，中間注意觀察，我們的技術人員會一直跟蹤指導，直到問題解決。

細胞培養代理品牌

細胞培養代理品牌

【虹膜色素上皮細胞總DNA】齊一生物科技（上海）有限公司是一家專業生產和銷售各種生化試劑,細胞株、原代細胞、菌種、醫藥中間體,標準品和對照品的大型化學科技公司. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490；Web:www.qiyibio.com

9236417  Software, Twister I, TWI            CNY                                  

9236264  Elution Microtubes Adaptor, inject mold              CNY                                  

9236165  O-ring, lubrication kit, RapidPlate         CNY                                  

9235931  Splashing guard, BR8 RD             CNY                                  

9230822  Rack for reagents,Trough (for 6 troughs)           CNY                                  

9235707  Washer Cable, RCS1             CNY                                  

9235695  Ground Wire, RCS1              CNY                                  

9235332  Z-Rack Packing Cover          CNY                                  

9235118  Fuse F1A, v1.00           CNY                                  

9235114  Fuse FST4A, v1.00              CNY                                  

9230054  Clamp, Tubing, Z-bar           CNY                                  

9234861  Lid coupling piece for system liquid             CNY                                  

9239551  Cbl Assy, XY, LED Driver            CNY                                  

9238663  Hook, motorized lid lock              CNY                                  

9238642  Capacitor board             CNY                                  

9238626  Opto-Encoder        CNY                            【虹膜色素上皮細胞總DNA】  

9238625  Eprom ａnd controller 4K15, Zent3C            CNY                                  

9241828  Pyro Q96 MDa, Premium Agreement    "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"      CNY                                  

9241122  Superlube              CNY                                  

9241007  QIArent&go - Rent out fee QIAcube           CNY                                  

9240985  PCB, drive, cooling device, Qsym        CNY                                  

9240982  Sensor, x, sample tracking, Qsym        CNY                                  

9240951  Support, cable, portal, Qsym        CNY                                  

9234820  Manual, user, QS 4.0, Int             CNY                                  

9240768  Signal Conditioner, Centrifuge, AP96           CNY                                  

9240764  DC Output Module 8NPT, 3AMP, AP96              CNY                                  

6000-5003      Duraseal Sealing Film            CNY                                  

9241781  RGQ, Full Agreement, no PM       "Repair service delivery: send to Regional Repair Center

Service response time (repair): 7-10 days turnaround time

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: yes, included (provision time 2 days)

Transportation cost coverage: yes, included"       CNY                                  

9241776  QIAgility, Inspection Service "The Annual Inspection Service, QIAgility is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAgility are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report." CNY                                  

9241756  IQ/OQ Services, QIAsymphony    Instrument qualification support service for the QIAsymphony. The product contains the Installation Qualification (IQ)/Operation Qualification (OQ) documentation ａnd the performance of the qualification protocols (travel ａnd labor costs included).      CNY                                  

9237126  Upgrade App., PCR Setup            CNY                                  

9237093  Training, Extension day  Additional days of training may be purchased during the week of the site visit upon request.       CNY                            【虹膜色素上皮細胞總DNA】  

9236426  Board, arm interconnect, TWI             CNY                                  

QY-x1172       人乳腺導管癌細胞MDA-MB-453                                         

QY-x1173       人乳腺細胞HBL-100                                      

QY-x1174       人乳腺癌細胞BCaP-37                                   

QY-x1175       人乳腺癌細胞LCC1                                       

QY-x1176       人乳腺癌細胞MX-1                                       

QY-x1177       人乳腺管癌細胞T47D                                    

QY-x1178       人乳腺癌細胞SK-BR-3                                    【虹膜色素上皮細胞總DNA】

QY-x1179       人乳腺癌細胞4T1                                   

QY-x1180       人乳腺癌細胞HCC1937                                         

QY-x1181       人乳腺導管癌細胞HCC38                                     

QY-x1182       人乳腺癌細胞Hs-578T                                   

QY-x1183       人乳腺癌Tamoxifen耐藥株LCC2                                        

QY-x1184       人乳腺癌抗雌激素耐藥株LCC9                                    

QY-x1185       人乳腺癌阿霉素耐藥細胞株MCF/Adr                                         

QY-x1186       人乳腺癌細胞BT-549                                     

QY-x1187       人乳腺導管瘤細胞BT-474                                     

QY-x1188       人乳腺癌細胞MB-271                                    

QY-x1189       人乳腺癌細胞ER-30                                      

QY-x1190       人乳腺癌細胞M453                                       

QY-x1191       人乳腺浸潤性導管癌旁皮膚細胞CCD-1095Sk                                   

QY-x1192       人乳腺細胞HBL-100                                      

QY-x1193       人乳腺導管瘤細胞UACC812                                        

QY-x1194       人乳腺癌細胞系1590                                      【虹膜色素上皮細胞總DNA】

QY-x1195       人卵巢癌細胞A2780                                      

QY-x1196       人卵巢癌細胞Sw626                                     

QY-x1197       人卵巢癌順鉑耐藥株COC1/CDDP                                       

QY-x1198       人卵巢癌紫杉醇耐藥株A2780/Taxol                                    

QY-x1199       人卵巢癌細胞系Anglne                                         

QY-x1200       人卵巢癌細胞OVCaR-3                                         

QY-x1201       人卵巢癌細胞Caov-3                                     

QY-x1202       人卵巢癌細胞HO-8910