高效：在所有細胞中實現RNA遞送

即用型：無需額外的緩沖液

低核酸量：毒性小化

保護 RNA 免受降解

與任何培養基兼容

血清兼容：無需更換培養基

RmesFect™ CRISPR transfection Reagent based on the TEE-technology is specifically designed for mRNA/gRNA transfection with high efficiency ａnd low toxicity. RmesFect™ is efficient in a large variety of cells.

Benefits:

Highly efficient: achieve RNAs delivery in all cells

Ready-to-use: no need for additional buffer

Low nucleic acid amount : minimized toxicity

Protects RNAs against degradation

Compatible with any culture medium

Serum compatible : Medium changed not required

與質粒DNA遞送相比，mRNA轉染具有以下優勢：

無需核吸收。mRNA翻譯成蛋白質發生在細胞質中。

蛋白表達速度快于DNA轉染

無基因組整合

以完全啟動子非依賴的方式表達蛋白質

RmesFect CRISPR轉染試劑結果示意圖：

根據標準方案，使用RmesFect CRISPR用編碼GFP和RFP的mRNA（各0.25µg）轉染DC24細胞。轉染后24小時在顯微鏡熒光下分析圖像。

來源：https://www.amyjet.com/products/OZB-RMC70500.shtml