用于控制質粒DNA或siRNA遞送的生物材料可以提供靶向轉基因表達（過度表達或阻斷）的基本工具，將為基因或細胞治療提供新的視角。艾美捷 OZ Biosciences-3D細胞培養用轉染試劑，針對基質膠和水凝膠優化！

3D基質類型：

艾美捷 3D細胞培養支架- 3D Scaffolds(#TF20250)

Collagen-based Scaffolds Collagen-derived Scaffolds Hyaluronic Acid MillicellTM (PTFE) Cell culture ｉnsert (Millipore) PCL，Polycaprolactone PEG，Poly(Ethylene Glycol) PLGA，Poly(lactic-co-glycolic acid) PS，Poly(Styrene) PU，Poly(Urethane)

3D水凝膠- 3D Hydrogels

Matrigel（BD）Collagen-Based Hydrogels Collagen-Derived Hydrogels Hyaluronic Acid Gelatin Extracellular Matrix Fibrin / Fibronectin Fibrinogen Laminin PEGylated hydrogels

發表文章：

細胞名稱 核酸類型 產品 參考文章

CLU-301 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

COS-1 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

COS-7 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HEK-293 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HeLa 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HMEC-1 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

MDCK 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

MEF 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

Neural Stem Cells 3D Transfection DNA 3D-Fect Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

RWPE1 siRNA 3D-Fect Laperrousaz B., Nucleic Acids Res. 2018 Jan 31. doi: 10.1093/nar/gky030.

3T3 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

AdSC 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

BMSC 3D Transfection DNA 3D-FectIN Sukarto A., Eur Cells ａnd Mat. 2011; 22(4):28.

Chondrocytes 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

COS-1 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

COS-7 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HeLa 3D Transfection siRNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HMEC-1 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

Mesenchymal Stem Cells mRNA 3D-FectIN Ledo AM. Acta Biomater . 2020 May 15;S1742-7061(20)30224-5.

Mesenchymal Stem Cells mRNA 3D-FectIN Ledo AM., Biomaterials. 2020 Jul;247:120016.

Mesenchymal Stem Cells 3D Transfection DNA 3D-FectIN Plank C., Eur. J. Nanomed. 2012;4(1):17–32

Mesenchymal Stem Cells 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

Neural Stem Cells 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

NIH-3T3 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

RAW 264.7 3D Transfection DNA 3D-FectIN Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

RWPE1 siRNA 3D-FectIN Laperrousaz B., Nucleic Acids Res. 2018 Jan 31. doi: 10.1093/nar/gky030.

ATDC5 siRNA si3D-FectIN Mori H., Am J Pathol. 2015 Aug 10. pii: S0002-9440(15)00385-5.

Chondrocytes siRNA si3D-FectIN Mori H., Am J Pathol. 2015 Aug 10. pii: S0002-9440(15)00385-5.

3D-Fect Transfection Reagent

門開發用于直接轉染在 3D 支架中培養的細胞。用3D-Fect / DNA復合物激活的基于支架的3D矩陣被細胞定植，隨后在更自然的環境中原位轉染。該方法可以研究組織工程和再生，腫瘤侵襲，神經分化，細胞化，組織形成，定植，神經突生長...   

結果展示：

3D-Fect 允許將多種細胞（細胞系和原代細胞）轉染到 3D 支架中：基于膠原蛋白和膠原蛋白衍生的支架預加載由 3D-Fect（比例 4：1）和 1 μg pVectOZ-GFP（綠色圖片）或 RFP 質粒（紅色圖片）形成的復合物。然后接種細胞并在48小時后在熒光顯微鏡下觀察

來源：https://www.amyjet.com/featured/3d-fect-transfection.shtml