Information

Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, ａnd freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.

Usage

Physical State: Freeze-dried solid

Storage ａnd Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) ａnd centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.

Extended Storage after Rehydration: Aliquot ａnd freeze undiluted product at -20°C or below. Avoid repeated freezing ａnd thawing.

Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.

017-000-121正常驢血清（艾美捷）中文說明：

正常血清經脂質提取以提高透明度，用含疊氮化鈉的磷酸鹽緩沖鹽水（PBS）透析，然后冷凍干燥。強烈推薦在PBS中稀釋至5%（v/v）的正常血清作為阻斷試劑，以減少非特異性、保守序列和/或Fc受體結合的背景。在添加一抗體之，使用來自與標記抗體相同的宿主的稀釋的正常血清作為單獨的孵育步驟獲得佳結果。

艾美捷017-000-121正常驢血清基本參數：

Host: Donkey

Product:

Conjugate: Unconjugated

Product Category: Normal Serums ａnd Gamma Globulins

RRID: AB_2337258

注意：

1、017-000-121正常驢血清可應用于大多數免疫學實驗，用作封閉液或空白對照.

2、凍干粉末形式，保存在2-8°C，準備用時，根據指示的體積加入雙蒸水，在2-8°C條件下，未稀釋的液體保質期6周，每天準備新鮮的工作液.如果重溶后，想延長保存期，將未稀釋的溶液保存在-20°C或者更低，避免反復凍融.

來源：https://www.amyjet.com/products/017-000-121.shtml