FAM FLICA Caspase-1探針允許研究人員評估Caspase-1的激活。FLICA試劑FAM-YVAD-FMK進入每個細胞，并不可逆地與活化的caspase-1結合。因為FAM-YVAD-FMK FLICA試劑與活性酶共價偶聯，所以它保留在細胞內，而任何未結合的FAM-YVAD-FMK-FLICA試劑擴散出細胞并被沖走。剩余的綠色熒光信號是添加試劑時細胞中存在的活性caspase-1酶活性的直接測量。含有結合的FLICA的細胞可通過熒光平板讀取器、熒光顯微鏡或流式細胞儀進行分析。用FLICA試劑標記的細胞可立即讀取或使用試劑盒中的固定劑保存16小時。還可以用碘化丙啶或Hoechst 33342分析未固定的樣品，以分別檢測核形態的壞死或變化。

艾美捷ICT FLICA天冬氨酸蛋白酶Caspase-1活性檢測試劑盒參數：

試劑名稱FAM-YVAD-FMK

目標Caspase-1

激發/發射488nm/530 nm

分析方法流式細胞儀、熒光顯微鏡、熒光板讀取器

樣品類型細胞培養

儲存2-8°C

艾美捷ICT FLICA天冬氨酸蛋白酶（Caspase）活性檢測試劑盒組分：

Kit 97: 25 Tests

FLICA Caspase-1 Reagent (FAM-YVAD-FMK), 1 vial, #653

10X Apoptosis Wash Buffer, 15 mL, #635

Fixative, 6 mL, #636

Propidium Iodide, 1 mL, #638

Hoechst 33342, 1 mL, #639

Kit Manual

Kit 98: 100 Tests

FLICA Caspase-1 Reagent (FAM-YVAD-FMK), 4 vials, #653

10X Apoptosis Wash Buffer, 60 mL, #634

Fixative, 6 mL, #636

Propidium Iodide, 1 mL, #638

Hoechst 33342, 1 mL, #639

Kit Manual

天冬氨酸蛋白酶（Caspase）活性檢測試劑盒部分文獻參考：

Hu, R;Liang, J;Ding, L;Zhang, W;Liu, X;Song, B;Xu, Y. Edaravone dexborneol provides neuroprotective benefits by suppressing NLRP3 inflammasome-induced microglial pyroptosis in experimental ischemic stroke. International immunopharmacology. 2022 October 21; doi: 10.1016/j.intimp.2022.109315. Article

Yuan, X;Bhat, OM;Zou, Y;Li, X;Zhang, Y;Li, PL. Endothelial Acid Sphingomyelinase Promotes NLRP3 Inflammasome ａnd Neointima Formation during Hypercholesterolemia. Journal of lipid research. 2022 October 14; doi: 10.1016/j.jlr.2022.100298. Full Text

Wan, Y;Zhang, W;Huang, C;Jian, J;Zhang, Y;Liu, Q;Chen, P;Zhu, X. Ursolic acid alleviates Kupffer cells pyroptosis in liver fibrosis by the NOX2/NLRP3 inflammasome signaling pathway. International immunopharmacology. 2022 October 14; doi: 10.1016/j.intimp.2022.109321. Text

來源：https://www.amyjet.com/products/ICT-97.shtml