艾美捷Immunochemistry 天冬氨酸蛋白酶Caspase-1活性檢測試劑盒原理：

FLICA試劑FAM-YVAD-FMK（Ex/Em = 488nm/530nm），是無細胞毒性的，細胞膜滲透抑制劑。進入每個細胞，并不可逆地與活化的caspase-1結合。任何未結合的FAM-YVAD-FMK-FLICA試劑擴散出細胞并被沖走。因此留在細胞內的綠色熒光直接體現了活性caspase-1酶活性的。熒光結果可通過熒光酶標儀、熒光顯微鏡、流式細胞儀進行分析。

艾美捷Immunochemistry 天冬氨酸蛋白酶Caspase-1活性檢測試劑盒組分：

25T：

l FLICA Caspase-1 Reagent (FAM-YVAD-FMK), 1 vial, #653 l 10X Apoptosis Wash Buffer, 15 mL, #635 l Fixative, 6 mL, #636 l Propidium Iodide, 1 mL, #638 l Hoechst 33342, 1 mL, #639 l Kit Manual

100T：

l FLICA Caspase-1 Reagent (FAM-YVAD-FMK), 4 vials, #653 l 10X Apoptosis Wash Buffer, 60 mL, #634 l Fixative, 6 mL, #636 l Propidium Iodide, 1 mL, #638 l Hoechst 33342, 1 mL, #639 l Kit Manual

保存條件：2-8°C 避光保存。

FAM-FLICA Caspase-1 活性分析試劑盒Nature文章鑒賞：

IF=30.9, 《Escherichia coli Rho GTPase-activating toxin CNF1 mediates NLRP3 inflammasome activation via p21-activated kinases-1/2 during bacteraemia in mice》,Nature Microbiology

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