線粒體膜電位的喪失表明細胞凋亡。ICT的MitoPT JC-1測定利用電位熒光染料JC-1檢測線粒體膜通透性和膜去化。JC-1染料具有分散在整個分子結構中的離域正電荷。此外，其親脂性溶解性使其易于滲透膜并穿透活細胞。JC-1染料進入帶負電荷的線粒體，在那里聚集并在激發時發出橙色熒光。當凋亡細胞中線粒體膜電位崩潰時，MitoPT JC-1分布在整個細胞溶膠中。使用流式細胞儀、熒光平板讀取器或熒光顯微鏡分析結果。

ImmunoChemistry艾美捷MitoPT JC-1檢測試劑盒化學性質：

試劑名稱JC-1

靶向線粒體去化

激發/發射488 nm/590 nm和527 nm

分析方法流式細胞術、熒光顯微鏡、熒光板讀取器

樣品類型細胞培養

儲存-20°C

試劑盒組分：

Kit 911: 400 Tests

MitoPT JC-1 Reagent, 100 Tests, #6260

10X Assay Buffer, 125 mL, #6259

CCCP, 50 mM, 600 µL, #6258

Kit Manual

Kit 924: 100 Tests

MitoPT JC-1 Reagent, 100 Tests, #6261

10X Assay Buffer, 60 mL, #685

CCCP, 50 mM, 125 µL, #6257

Kit Manual

ImmunoChemistry艾美捷MitoPT JC-1檢測試劑盒相關文獻：

Funakoshi, A;Honda, T;Ito, T;Tokura, Y. Cholecystokinin receptor antagonist suppresses melanoma growth by inducing apoptosis of tumor cells. JID Innovations. 2022 September 1; doi: 10.1016/j.xjidi.2022.100153. Abstract

Yoshioka, H;Komura, S;Kuramitsu, N;Goto, A;Hasegawa, T;Amizuka, N;Ishimoto, T;Ozasa, R;Nakano, T;Imai, Y;Akiyama, H. Deletion of Tfam in Prx1-Cre expressing limb mesenchyme results in spontaneous bone fractures. Journal of bone ａnd mineral metabolism. 2022 August 10; doi: 10.1007/s00774-022-01354-2. Publication

Zhong, G;Long, H;Zhou, T;Liu, Y;Zhao, J;Han, J;Yang, X;Yu, Y;Chen, F;Shi, S. Blood-brain barrier Permeable nanoparticles for Alzheimer＇s disease treatment by selective mihagy of microglia. Biomaterials. 2022 August 8; doi: 10.1016/j.biomaterials.2022.121690. Full Text

ImmunoChemistry Technologies簡稱"ICT",提供各種細胞蛋白酶、細胞凋亡與細胞毒性檢測試劑盒等研究工具，同時包含高質量高靈敏度的 ELISA開發試劑。艾美捷科技是ImmunoChemistry的中國代理商，為科研工作者提供優質的產品與服務。

來源：https://www.amyjet.com/brand/Immunochemistry.shtml