EndoTrap is an affinity chromatography resin for the efficient removal of bacterial endotoxins

(lipopolysaccharides/LPS) from aqueous solutions containing low or high molecular weight substances such as proteins ａnd nucleic acids. EndoTrap can be employed both in column or batch mode, by gravity flow or on fully automated liquid chromatography systems.

The EndoTrap family contains two members:

EndoTrap HD

with a broad pH ａnd salt tolerance, works best with calcium or magnesium compatible buffers such as TRIS, HEPES, MOPS but also PBS, if enriched freshly with 1 mM Ca/Mg2+ ａnd 1 mM Citrate.

Furthermore, it was particularly developed for challenging samples ａnd large scale endotoxin

removal in e.g biopharmaceutical production processes. For EndoTrap HD, a Regulatory Support File (RSF) ａnd an EndoTrap leakage ELISA are available additionally.

EndoTrap red

especially for the use with buffers containing calcium chelators.

This multipurpose product portfolio covers a broad range of conditions with no special equipment or buffer requirements. EndoTrap HD 1/1 ａnd 5/1 as well as all EndoTrap red kits include all buffers needed. EndoTrap HD is supplied including instructions for buffer preparation ａnd can be supplied including buffers on request.

The following tables give a short overview on the differences of the EndoTrap systems.

艾美捷內毒素純化樹脂，是一種用于去除生物源蛋白類產品(包括多肽、抗體、多糖等)中內毒素的樹脂。其原理是將修飾過的多粘菌素B連接至4%瓊脂糖微球上，進行特異性去除內毒素，經純化可將樣品中內毒素降低至0.1EU/ml，且樣品回收率高。

艾美捷內毒素純化樹脂特點：

1、該內毒素純化樹脂滿足工業應用的所有要求，并且可以輕松集成到/在早期或后期純化過程中。

2、High-DefinitionMatrix還可作為預填充柱用于小規模純化。

3、在4-9的pH值范圍和50-600mM的NaCl濃度范圍內，使用EndoTrap®HD去除內毒素有效。

4、快速無孵育步驟–真正的流通系統，J佳蛋白質回收率將樣品損失降至低，樣品回收率高達99%，LPS變異性廣泛菌株的內毒素去除，可重復使用/再生可重復使用至少三次，EndoTrap HD至少10次，內毒素去除效率損失；-包括再生緩沖液，再生物質無毒，無脫氧膽酸鹽（DOC）!

*建議使用HEPES、TRIS、MOPS、MES和PIPES等緩沖液。必須避免使用檸檬酸鹽緩沖液和二價陽離子螯合劑（如EDTA）

艾美捷內毒素純化樹脂EndoTrap HD和EndoTrap-red規格比較：

來源：https://www.amyjet.com/products/LET0023.shtml