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Intended Uses:
Enzyme Immunoassay for the quantitative determination of L-Kynurenine in serum, plasma ａnd various biological samples. After acylation Kynurenine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls ａnd samples ａnd the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen ａnd free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction Is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.
Principle of the Assay:
Enzyme Immunoassay for the quantitative determination of L-Kynurenine in serum, plasma ａnd various biological samples. After acylation Kynurenine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls ａnd samples ａnd the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen ａnd free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction Is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.
艾美捷 MyBioSource犬尿氨酸 ELISA試劑盒是種即用型微孔板或全板 ELISA（酶聯免疫吸附測定）試劑盒，用于分析生物樣品中犬尿氨酸、ELISA 試劑盒目標分析物的存在。試劑盒標準品或陽性對照的濃度梯度為含有犬尿氨酸的生物研究樣品提供了理論上的試劑盒檢測范圍。
艾美捷 MyBioSource犬尿氨酸 ELISA試劑盒測定原理：
酶免疫測定法用于定量測定血清、血漿和各種生物樣品中的 L-犬尿氨酸。�；笕虬彼嵬ㄟ^ELISA定量測定。競爭性 ELISA 使用微量滴定板格式�？乖c微量滴定板的固相結合。�；臉藴势�、對照品和樣品與固相結合的分析物競爭固定數量的抗體結合位點。當系統處于平衡狀態時，通過洗滌除去游離抗原和游離抗原-抗體復合物。通過使用 TMB 作為底物的抗兔 IgG-過氧化物酶偶聯物檢測與固相結合的抗體。在 450 nm 監測反應。未知樣品的定量是通過將它們的吸光度與用已知標準制備的參考曲線進行比較來實現的。
艾美捷 MyBioSource犬尿氨酸 ELISA試劑盒典型檢測數據/標準曲線（僅供參考）：
相關參考文獻：
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Increased depressive ratings in patients with hepatitis C receiving interferon-alpha-based immunotherapy
are related to interferon-alpha-induced changes in the serotonergic system. J Clin Psychopharmacol,
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(3) Bryleva, E. Y., & Brundin, L. (2017). Kynurenine pathway metabolites ａnd suicidality.
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