齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1。我必須運行所有的標準和樣品的重復？

是的，重復的運行，以監測測定的精度和提高檢測結果的信心。

2。我必須在一次運行所有的樣品嗎？

不，每個試劑盒采用stripwell微孔板。這允許用戶在不同的時間來分析不同數量的樣品。

3。什么類型的可重復性的結果，得到與分析？

每一個工具都有一個包含一個典型數據的圖表的手冊。在運營商的任何變化，移液和洗滌技術，培養時間、溫度、試劑盒的年齡可以引起變化的結果。每個用戶都應該得到自己的標準曲線。

4。是否有可能儲存的試劑比表示？

在不推薦使用條件下的部件組件的存儲，以保證測試的正確性能。

5。我應該如何儲存我的樣品？

樣品應保存在-20°C或更低的溫度。長期儲存，建議凍結他們在- 70余。

6。我可以修改協議嗎？

BG的ELISA試劑盒進行了優化，提供*佳的結果。修改的格式或協議可能會給出錯誤的結果。

7。我可以使用一個不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗證在試劑盒插入的樣品類型。未經檢驗的樣品類型。為進一步信息的聯系技術服務。

8。我的樣本產生的值，在動態范圍內的測定。我可以使用這些值嗎？

建議只有在標準曲線的范圍內下降的樣本值。標準曲線的范圍內的值一般是非線性的，這可能導致不正確的外推值。產生值高于標準的樣品應為（進一步）稀釋和重復。如果樣品低于該測定的范圍內，該樣品被認為是不可檢測的。

9。我每次都要運行一個空白或零標準嗎？

是的，這些都是必需的計算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測到檢測。當排除特定的檢測問題的來源時，它們也非常有幫助。

10。我能改變我在檢測中使用的樣本量嗎？

不建議你改變卷由于BG工具被設計為*佳的性能在給定的體積

11�？梢允褂貌煌慕M件組件嗎？

每一個組件包含有特定批號的組件，以確保所有的組件都進行優化，以及與組件中的所有其他組件。對這些特定的地段進行質量檢驗。這是從來沒有建議使用你自己的組件或組件從其他包或供應商。

12。我的標準曲線看起來很好，但我沒有得到一個信號，我的樣本，當我預期，為什么？

樣品不包含被分析物。一個矩陣的效果可能是掩蓋檢測。確保推薦的稀釋液在試劑盒插入后按規定進行。如果建議稀釋，請檢查是否正確進行稀釋。過稀釋會導致樣品在標準曲線的范圍內下降。

13。你怎么推薦我洗盤子？

如果您使用的是一個自動平板洗衣機，我們建議定期檢查校準，并將該系統在洗滌前沖洗緩沖液沖洗。手動洗衣機也是一樣的。一個中繼器或洗瓶也可以使用。用戶應小心，以確保所有的內容都是送氣和板拍干的無塵紙。【人血清血管緊張素Ⅵ（ANGⅥ）ELISA試劑盒現貨】

14。我需要用一個盤子嗎？

可靠的結果可以得到無板振動，但外徑的一般會低于那些使用平板振動器。

15。為什么我要用波長校正450-570nm之間？

對于酶聯免疫吸附試驗，在雙波長讀的是做正確的光密度貢獻的塑料以及，燈和光學波動。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預期的分析物濃度，我應該找到？

一個給定的分析物的量可能會有所不同，不僅從物種的物種，但也組織和細胞來源。這個信息的*佳來源是目前的文獻，很容易通過互聯網在多個科學數據庫訪問。

18。我的光密度有點高（或較低），比那些在手冊中，與我的試劑盒來了。為什么？

光學密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【人血清血管緊張素Ⅵ（ANGⅥ）ELISA試劑盒現貨】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【人血清血管緊張素Ⅵ（ANGⅥ）ELISA試劑盒現貨】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【人血清血管緊張素Ⅵ（ANGⅥ）ELISA試劑盒現貨】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

9022021  Purge line (incl. tubes & fittings), QXA              CNY                             

9238537  Sealing ring, auto vac base, BR8000                 CNY                             

9238011  Calibration, Meter, Tension, Sonic, 505C                   CNY                              【人血清血管緊張素Ⅵ（ANGⅥ）ELISA試劑盒現貨】

19054       Buffer QBT       1000 ml Equilibration Buffer    CNY                             

10023       QIAGEN-tip 20 (25)  25 columns; 20 µg column capacity         CNY                             

133284     NeXtalStock TRIS pH 8.0 (200)                   CNY                             

4503163  artus CMV TM PCR Kit (24) CE                   CNY                             

4502363  artus VZV QS-RGQ Kit (24) CE           CNY                             

4502263  artus VZV RG PCR Kit (24) CE            CNY                             

133261     NeXtalStock NVH Oil (200)                CNY                             

990556     Reagent Trough Lid 170 mL (20)               CNY                             

990552     Tubes, graduated, flat-base 5 ml (50)               CNY                             

990550     Tip Disposal Box (10)                 CNY                             

990532     50µl Filtered Sterile Tips (960)                  CNY                             

990452     Filter-Tips, 1000 µl wide bore (1024)                CNY                             

337629     qBiomarker SYBR Green Fluor Mastermix 20           CNY                             

337012     FDK design fee          CNY                             

4501265  artus EBV RG PCR Kit (96) CE            CNY                             

4501103  artus EBV TM PCR Kit (24) RUO                CNY                              【人血清血管緊張素Ⅵ（ANGⅥ）ELISA試劑盒現貨】

949014     Autopure RNase A Solution (19 ml) "RNAse A for Gentra/Puregene products.

Concentration is 4 mg/ml"       CNY                             

990352     Filter-Tips, 1000 µl (1024)                 CNY                             

331401     RT² miRNA First Strand Kit (12)                 CNY                             

4500063  artus HSV-1/2 LC PCR Kit (24) CE                CNY                             

1070216  Gauge (1)                   CNY                             

9012944  Liquid Handling Kit Plato 3000 (1000ul)            CNY                             

9012916  Magnet, 96-well, Type B          CNY                             

9012806  Tube Holder, STS, cryotubes, 2ml (6)                 CNY                             

937055     QIAsymphony DSP Virus/Pathogen Midi Kit             CNY                             

931255     QIAsymphony DNA Midi Kit (96)               CNY                             

9010573  Tischplatte PK80 (1177)            CNY                             

979102     PyroMark Q96 HS Q96 Reagent Tip (4)   Re-usable tips for dispensing reagents (RDTs) for use in PyroMark Q96 MD. Each package contains 4 tips.  CNY                             

QY-14252 Anti-HMGA2 (high mobility group A2)高遷移率族蛋白A2抗體                                                

QY-14262 Anti-HMGB1 (High Mobility Group Box protein 1)高遷移率族蛋白-1抗體                                                

QY-14272 Anti-HO-1/HSP32 (Hemeo xygenase 1)血紅素氧合酶-1/熱休克蛋白-32抗體                                                    

QY-14282 Anti-HO-2 （Heme oxygenase 2）血紅素氧合酶-2抗體                                                  

QY-14292 Anti-HOXc8同源盒基因的一種                                                   

QY-14302 Anti-HPV (Human papillomavirus)人類乳頭狀瘤病毒抗體                                                  【人血清血管緊張素Ⅵ（ANGⅥ）ELISA試劑盒現貨】

QY-14312 Anti-H-ras原癌基因H-ras抗體                                                   

QY-14322 Anti-HSL(hormone-sensitive lipase)荷爾蒙敏感脂肪酸抗體                                              

QY-14332 Anti-HSP-27(Heat Shock Protein 27)熱休克蛋白-27抗體                                                    

QY-14342 Anti-HSP-60（ Heat shock protein-60）熱休克蛋白-60抗體                                                    

QY-14352 Anti-HSP-70 (Heat shock protein-70)熱休克蛋白-70抗體                                                   

QY-14362 Anti-HSP-90α(Heat Shock Protein 90α )熱休克蛋白90α抗體                                                       

QY-14372 Anti-HSP-90α(Heat Shock Protein 90α )熱休克蛋白90α抗體                                                       

QY-14382 Anti-HSP-90β （heat sock protein-90β）熱休克蛋白-90β 抗體                                                 

QY-14392 anti-HtrA2/Omi(mitochondrial intermembrane space)絲氨酸蛋白酶/線粒體絲氨酸蛋白酶抗體                                                   

QY-14402 Anti-hTRTTelomerase catalytic subunit（TE）端粒酶抗體（催化亞單位）                                                     

QY-14412 Anti-Human serumAnti-Human serum兔抗人血清抗體抗血清                                                 

QY-14422 Anti-Hydroxyethyk starch/HES Anti-hydroxyethyk starch/HES羥yi基淀粉抗體                                                

QY-14432 Anti-Hypothetical protein （LOC55652）hypothetical protein抗體                                                  

QY-14442 anti-IAA（Indole-3-Acetic Acid）吲哚-3-乙酸抗體/植物生長素抗體                                               

QY-14452 anti-IAA（Indole-3-Acetic Acid）吲哚-3-乙酸抗體/植物生長素單克隆抗體                                                    

QY-14462 anti-IAP-5（inhibitors of apoptosis protein-5）凋亡抑制因子5抗體                                                 【人血清血管緊張素Ⅵ（ANGⅥ）ELISA試劑盒現貨】

QY-14472 Anti-IASPP(inhibitory member of the ASPP family)腫瘤細胞凋亡ASPP家族的另一個成員抗體                                                      

QY-14482 Anti-ICAD（Inhibitor of Caspase-Activated Dnase ）Caspase 激活的脫氧核糖核酸酶抑制劑                                              

QY-14492 Anti-IDE(Insulin degradation enzyme)胰島素降解酶抗體                                                  

QY-14502 Mouse Anti-influenza A virus nucleoprotein Monoclonal（Flu-A） 鼠抗人禽流感A核蛋白單克隆抗體                                                

QY-14512 Mouse Anti-influenza B virus nucleoprotein Monoclonal（Flu-B）鼠抗人禽流感B核蛋白單克隆抗體                                                  

QY-14522 Anti-IFABP（Intestinal Fatty acid binding protein）小腸型脂肪酸結合蛋白抗體                                                     

QY-14532 Anti-IFITM1 (interferon induced transmembrane protein 1)干擾素誘導跨膜蛋白1抗體                                                

QY-14542 Anti-IFN-β (Interferon β)Anti-human干擾素-β抗體（抗人）                                                     

QY-14552 Anti-IFN-β (Interferon β)Anti-mouse,rat干擾素-β抗體（抗大鼠、小鼠）                                              

QY-14562 Anti-IFN-γ(Mouse Anti-Human Interferon γ Monoclonal Antibody）鼠抗人干擾素-γ/IFN-gamma單克隆抗體                                                       

QY-14572 Anti-IFN-γ(Interferon γ)Anti-Human干擾素-γ抗體（抗人）                                                      

QY-14242       Anti-HMGA1 (high mobility group AT-hook 1)高遷移率族蛋白A1抗體