產品展示
人α乳清蛋白(α-La)ELISA試劑盒廠家
點擊次數：17發布時間：2016/8/17 16:48:23
更新日期：2016/8/17 16:48:23
所 在 地：中國大陸
產品型號：
優質供應
詳細內容
ELISA 檢測的目的是為實驗提供準確可靠的定量分析實驗依據。為了保證實驗數據的可靠性，在實驗過程中必須堅持全面質量控制和全過程質量控制。在收集標本前都必須有一個完整的計劃。齊一生物銷售：021－6034 8496；181214 53965；173021 04490
注意事項：
◆ 每個標本量收集體積＝ 100ul × 檢測種類，如要做復孔，標本量收集體積＝ 100ul × 檢測種類×2 。
◆ 對于作某一個具體指標來說，先用一系列稀釋度作一批標本測定，得出對實驗有意義的一個稀釋度（即測定劑量反應曲線），然后用一個*適稀釋度測定即可。
◆ 收集標本前必須清楚要檢測的成份是否足夠穩定。對收集后當天進行檢測的標本，儲存在4℃備用，如有特殊原因需要周期收集標本，將標本及時分裝后放在-20℃ 或-70℃ 條件下保存。避免反復凍融。標本 2 -8 ℃ 可保存48 小時.-20℃ 可保存1個月。 -70 度可保存6個月。部分激素類標本需添加抑肽酶。
◆ 血清標本采集時，應注意避免溶血，紅細胞溶解時會釋放出具有過氧化物酶活性的物質，以 HRP為標記的 ELISA 測定中，溶血標本可能會增加非特異性顯色。
【人α乳清蛋白(α-La)ELISA試劑盒廠家】◆ 為了保證尿液檢測結果的準確性，必須正確收集尿液標本和保存。盛尿容器要清潔干燥。使用一次性的容器（如塑料尿杯），避免因用藥并清洗不干凈而造成的污染，影響檢測結果。尿液標本必須新鮮，留取后，應及時檢測或保存，以免細菌繁殖。因在室溫中久置后（尤其是夏季）尿中磷酸鹽等可析出結晶而干擾檢測。
◆ 標本宜在新鮮時檢測。如有細菌污染，菌體中可能含有內源性 HRP，也會產生假陽性反應。保存過久可發生聚合在 ELISA中可使本底加深。
◆ 凍結標本融解后，蛋白質局部濃縮，分布不均，應充分混勻宜輕緩，避免氣泡，可上下顛倒混和，不要在混勻器上強烈振蕩。
◆ 混濁或有沉淀的標本應先離心或過濾，澄清后再檢測。
◆ 反復凍融會使蛋白效價降低，所以待測標本如需保存作多次檢測，宜少量分裝冰存。也可加入適當防腐劑。
◆ 如果您在實驗中遇到任何特殊問題，敬請咨詢 021-60348496標本類型
ELISA常見的標本一般包括：
◆ 液體類標本：包括血清、血漿、尿液、胸腹水、腦脊液、細胞培養上清等。
◆ 培養細胞
◆ 組織標本
這些標本收集的時間、方法和保存都有一定的要求。
一般說來，在 5 天內測定的標本可放置于 4℃，超過一周測定的需低溫凍存。
對收集后當天進行檢測的標本，儲存在 4 ℃ 備用，如有特殊原因需要周期收集標本， 將標本及時分裝后放在 -20 ℃ 或 -70 ℃ 條件下保存。避免反復凍融。 標本 2 -8 ℃ 可保存 48 小時，-20 ℃ 可保存 1 個月。-70 度可保存 6 個月。部分標本也可加入適當防腐劑，激素類標本需添加抑肽酶。標本的處理 可用作 ELISA 測定的標本十分廣泛，體液（如血清）、分泌物（唾液）和排泄物（如尿液）、細胞培養上清、細胞、組織等均可作標本以測定其中某種成份。有些標本可直接進行測定，有些則需經預處理。
◆ 血清：
室溫血液自然凝固 10-20 分鐘后，離心 20 分鐘左右（ 2000-3000 轉 / 分）。收集上清。如有沉淀形成，應再次離心。
◆ 血漿：
應根據試劑盒的要求選擇 EDTA 、檸檬酸鈉或肝素作為抗凝劑，加入 10 ％（ v/v ）抗凝劑 ( 0.1M 檸檬酸鈉或 1% heparin 或 2.0%EDTA.Na2) 混合 10-20 分鐘后，離心 20 分鐘左右（ 2000-3000 轉 / 分）。仔細收集上清如有沉淀形 成，應再次離心。
【人α乳清蛋白(α-La)ELISA試劑盒廠家】◆ 尿液、胸腹水、腦脊液：
用無菌管收集。離心 20 分鐘左右（ 2000-3000 轉 / 分）。仔細收集上清。如有沉淀形成，應再次離心。
◆ 細胞培養上清：
檢測分泌性的成份時，用無菌管收集。離心 20 分鐘左右（ 2000-3000 轉 / 分）。仔細收集上清。檢測細胞內的 成份時，用 PBS （ PH7.2-7.4 ）稀釋細胞懸液，細胞濃度達到 100 萬 /ml 左右。通過反復凍融，以使細胞破壞并放出細胞內成份。離心 20 分鐘左右（ 2000-3000 轉 / 分）。仔細收集上清。保存過程中如有沉淀形成，應再次離心。
◆ 細胞：
檢測細胞的成份時，對于貼壁細胞，去除培養液，用PBS、生理鹽水或無血清培養液洗一遍。加入適量裂解液。用槍吹打數下，使裂解液和細胞充分接觸。通常裂解液接觸細胞10 秒后，細胞就會被裂解。對于懸浮細胞，離心收集細胞，用PBS、生理鹽水或無血清培養液洗一遍.加入適量裂解液, 用槍吹打把細胞吹散。用手指輕彈以充分裂解細胞。充分裂解后應沒有明顯的細胞沉淀。如果細胞量較多，必需分裝然后再裂解。充分裂解后，10000-14000g離心3-5 分鐘，取上
清，即可進行后續操作。
【人α乳清蛋白(α-La)ELISA試劑盒廠家】◆ 組織標本：
切割標本后，稱取重量。加入一定量的 PBS或組織蛋白萃取試劑，緩沖液中可加入蛋白酶抑制劑。用手工或勻漿器將標本勻漿充分。離心 20 分鐘左右（ 2000-3000 轉 / 分）。仔細收集上清置于 -20 度或 - 70 度保存，如有必要，可以將樣品濃縮干燥。分裝后一份待檢測，其余冷凍備用。齊一生物銷售：021－6034 8496；181214 53965；173021 04490
9240651 Internal BCR, Keyence, BL-180, AP96/196 CNY
9240626 Fuse, 8amp, 5 X 20 mm, AP196/98 CNY
9240623 Circuit Breaker, 2A, AP196/98 CNY
9240591 3-Way Mac Valve, AP196/96 CNY
9240574 Micro-Lynx w\speedbaord, AP98 CNY
9240572 Rotor w/Buckets, Sorvall, AP96 CNY
9240552 Freq. Conv., PCB, Eppendorf, AP196/98 CNY
9240531 Cable, Motor, Axis #7, Pump U/D, AP98 CNY 【人α乳清蛋白(α-La)ELISA試劑盒廠家】
9240564 Fan, Condenser, 230V,Eppendorf, AP196/98 CNY
9240553 Cable, PC-104 to Eppendorf, AP196/98 CNY
9240548 Motor Cap Repair, Eppendorf, AP196/98 CNY
9240515 I/O PCB, Main Board, AP196/98 CNY
9240503 PM Kit, AP196 CNY
9239958 BR M48, PM Service "Service response time (repair): not applicable
Periodic inspection/maintenance: yes
Inspection/maintenance delivery: onsite
Cost coverage for Repair Parts : not included
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: yes, full coverage" CNY
9239853 Foot, complete, v2.0 (4) CNY
9239572 BR Universal, Full Agreement The Full Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days). CNY
9239563 Assy,Kit,Retrofit,HoriCompressorMnt CNY
9020033 LED UV (325), RGQ CNY
9020023 L 26_7 Firmware, DML2 CNY
9019975 PCB, UV light assembly, Cxx CNY
9238858 Basic Cover Agreement Plato7, 1yr The Basic Cover Agreement Plato7 provides full service coverage for the Plato 7 platform. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days. CNY
9241233 User Manual, QIAxcel FA 01/08 (WW) CNY
9241227 ZLV, Handscanner, Qsym CNY
9241180 Motor, Z, QX CNY 【人α乳清蛋白(α-La)ELISA試劑盒廠家】
9019473 Loading guide, 52 well sharkstooth, GSC CNY
9019462 Switch, micro, HS CNY
9019457 Fans (2), 5V, 30mm, HS CNY
9237172 Cable USB CNY
9233169 Board, YZ-CPU, BR8000 CNY
9237163 Bezel LiquiChip CNY
9237162 Shipping Plug XYP CNY
ULBC plates 超低結合培養板（6孔） Ultra-Low Binding Culture Plate (6-well) "6-well, 4 plates
" 【人α乳清蛋白(α-La)ELISA試劑盒廠家】
ULBC plate 超低結合培養板（24孔） Ultra-Low Binding Culture Plate (24-well) "24-well, 1 plate
"
ULBC plates 超低結合培養板（24孔） Ultra-Low Binding Culture Plate (24-well) "24-well, 4 plates
"
rhbFGF 重組人堿性成纖維細胞生長因子－－堿性 Recombinant Human Fibroblast Growth Factor-basic 10µg
rhEGF 重組人表皮生長因子 "Recombinant Human Epidermal Growth Factor
" 100µg
rhBMP-2 重組人骨形成蛋白-2 "Recombinant Human Bone Morphogenetic Protein-2
" 2µg
rhBMP-4 重組人骨形成蛋白-4 "Recombinant Human Bone Morphogenetic Protein-4
" 2µg
rhBMP-7 重組人骨形成蛋白-7 "Recombinant Human Bone Morphogenetic Protein-7
" 2µg
rhNOGGIN 重組人NOGGIN蛋白 "Recombinant Human NOGGIN
" 5µg
HAMSC 人羊膜間充質基質細胞 Human Amniotic Mesenchymal Stromal Cells 5 x 10^5 cells/vial
HCMSC 人絨毛膜間充質基質細胞 Human Chorionic Mesenchymal Stromal Cells 5 x 10^5 cells/vial
HMSC-bm 人間充質干細胞－－骨髓 Human Mesenchymal Stem Cells-bone marrow 5 x 10^5 cells/vial
HMSC-ad 人間充質干細胞－－脂肪 Human Mesenchymal Stem Cells-adipose 5 x 10^5 cells/vial
HMSC-hp 人間充質干細胞－－肝臟 Human Mesenchymal Stem Cells-hepatic 5 x 10^5 cells/vial
HUMSC 人臍帶間充質干細胞 Human Umbilical Mesenchymal Stem Cells 5 x 10^5 cells/vial
HPMSC 人肺間充質干細胞 Human Pulmonary Mesenchymal Stem Cells 5 x 10^5 cells/vial
HVMSC 人脊椎間充質干細胞 Human Vertebral Mesenchymal Stem Cells 5 x 10^5 cells/vial 【人α乳清蛋白(α-La)ELISA試劑盒廠家】
MSCM 間充質干細胞培養基 Mesenchymal Stem Cell Medium 500 ml
MODM-500 間充質干細胞-成骨細胞分化培養基 Mesenchymal Stem Cell Oseogenic Differentiation Medium 500 ml
MODM-250 Mesenchymal Stem Cell Oseogenic Differentiation Medium 250 ml
MODM-b 間充質干細胞-成骨細胞分化培養基-基礎 Mesenchymal Stem Cell Osteogenic Differentiation Medium-basal 500ml
MODM-b-prf 間充質干細胞-成骨細胞分化培養基-無酚紅 Mesenchymal Stem Cell Osteogenic Differentiation Medium-basal-phenol red free 500ml
MADM-500 間充質干細胞-脂肪細胞分化培養基 Mesenchymal Stem Cell Adipogenic Differentiation Medium 500 ml
MADM-250 Mesenchymal Stem Cell Adipogenic Differentiation Medium 250 ml
MADM-b 間充質干細胞-脂肪細胞分化培養基-基礎 Mesenchymal Stem Cell Adipogenic Differentiation Medium-basal 500 ml
MADM-b-prf 間充質干細胞-脂肪細胞分化培養基-無酚紅 Mesenchymal Stem Cell Adipogenic Differentiation Medium-basal-phenol red free 500ml
MCDM 間充質干細胞-軟骨分化培養基 Mesenchymal Stem Cell Chondrogenic Differentiation Medium 500ml
Ared 茜素紅S染色試劑盒 Alizarin Red S Staining Kit 100 ml
Ored 油紅O染色試劑盒 Oil Red O Staining Kit 100 ml
PCR 人間質干細胞成脂肪細胞檢測試劑盒 Human Mesenchymal Stem Cell Adipogenesis Detection Kit 50 reactions
HMSC-C PCR 人間充質干細胞軟骨形成檢測試劑盒 Human Mesenchymal Stem Cell Chondrogenesis Detection Kit 50 reactions
SafraninO 番紅O染色試劑盒 Safranin O Staining Kit 100 reactions
ABlue 阿利新藍染色試劑盒 Alcian Blue Staining Kit 100 reactions 【人α乳清蛋白(α-La)ELISA試劑盒廠家】
s�ci�*�)ily:"Times New Roman";Times New Roman"＇>人真皮成纖維細胞-胎兒C處理 Human Dermal Fibroblasts-Fetal-Mitomycin C treated 1 x 10^6 cells/vial
MEF-cm 小鼠胚胎成纖維細胞培養基 Mouse Embryonic Fibroblasts-Conditioned Medium 100 ml
bFGF-std MEF-cm 堿性成纖維細胞生長因子刺激的小鼠胚胎成纖維細胞條件培養基 bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium 100ml
ULBC plates 干細胞超低結合培養板 Ultra-Low Binding Culture Plate 6-well, 1 plate
l�K���*�)si-font-family:"Times New Roman";color:black;
5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6. The substrate evade the light preservation.
7. Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8. All samples, washing buffer ａnd each kind of reject should according to infective material process.
9. Do not mix reagents with those from other lots.
Calculate：
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
|
Storage ａnd validity
1．Storage： 2-8℃.
2．validity： six months.
9237124 Upgrade App., MinElute 96 Take advantage of our instrumentation ａnd molecular biology expertise to install MinElute 96 on your BioRobot System. The onsite protocol development visit includes all travel ａnd labor for the visit to install the standard QIAGEN MinElute 96 protocol, plus a MinElute 96 BioRobot Kit (4-plate) Cat # 28051 CNY
9237099 Upgrade BR configuration, on site CNY
9236424 Motor, rotary, TWI CNY
9232970 YZ 96 Connector Board 2 (Arm), E10103 CNY
9236409 Plate Stacker TW I, microplate CNY
9236357 Sample Holder Large, 1.5ml CNY 【人淀粉樣前體蛋白(βAPP)ELISA試劑盒廠家】
9232911 Board, YZ driver CNY
9236336 Analog Board CNY
9236158 Pot. , syr. or vert. , kit, RapidPlate CNY
9236149 Locator Plate, 96 well,pos 4-5,BR RP CNY
9236114 O-Ring, Coupling, RoboVac, BR8 RD v4.2 CNY
9236107 Flange Tool CNY
9230680 Probe, 0.4 mm, BR8000 v1.01 CNY
9230478 Rack f. Diamend Gel Cards (holds 6cards) CNY
9230473 Set, Cables, PCB (X-main) to shaker CNY
9235646 Connector, Board, HS shaker, BR8000 v1.0 CNY
9235255 T-piece, HSPS tubing, connector CNY
9235190 Motor, clamp, shaker plate, BR8000 CNY
9230060 Rack for reagents (12 x diameter 21mm) CNY
9238673 Hinge, imbalance system CNY
9238653 Power Board, temp. switch - heat sink CNY
9238635 Hook identification compl. CNY 【人淀粉樣前體蛋白(βAPP)ELISA試劑盒廠家】
9242028 RGQ, Repair Fee "Repair service delivery send to Regional Repair Center
Service response time (repair) 7-10 days turnaround time
Periodic inspection/maintenance: 0
Cost coverage for Repair Parts : yes, full coverage
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: yes, full coverage" CNY
9241821 Pyro Q96 MDa, Basic Agreement "Repair service delivery: onsite
Service response time (repair): 5 business days
Periodic inspection/maintenance: yes, 1/agreement year
Inspection/maintenance delivery: onsite
Cost coverage for Repair Parts: yes, included
Cost coverage for labor: yes, included
Cost coverage for travel: yes, included
Replacement system (Loaner) provision: not included
Transportation cost coverage: not applicable" CNY
9241786 QIAgility Demopool Box Light Version CNY
9240976 PCB, display, sample tracking, Qsym CNY
9240968 Barcode camera, sample tracking, Qsym CNY
9240816 #Full Agreement, EZ1 DSP, 1YR CNY
9240680 Spill Tray, Shaker,Modified, AP CNY
9240790 Update,Waste Bottle Sensor, AP196/98 CNY
6000-5014 Lumicheck Plate Service Package, DML2 CNY
TGS 滋養層細胞生長因子 Trophoblast Growth Supplement 5 ml
PAdDS 前脂肪細胞分化生長因子 Preadipocyte Differentiation Supplement
PAGS 前脂肪細胞生長因子 Preadipocyte Growth Supplement 5 ml 【人淀粉樣前體蛋白(βAPP)ELISA試劑盒廠家】
AdGS 脂肪細胞生長因子 Adipocyte Growth Supplement 5 ml
OEpiCGS 卵巢上皮細胞生長因子 Ovarian Epithelial Cell Growth Supplement 5 ml
MODS 間充質干細胞成骨分化生長因子 Mesenchymal Stem Cell Osteogenic Differentiation supplement 5 ml
MADS 間充質干細胞脂肪分化生長因子 Mesenchymal Stem Cell Adipogenic Differentiation supplement 5 ml
MSCGS 間充質干細胞生長因子 Mesenchymal Stem Cell Growth Supplement 5 ml
MSCGS-sf 間充質干細胞生長因子 Mesenchymal Stem Cell Growth Supplement-serum free 5 ml
MSCGS-acf 間充質干細胞生長因子-無動物組分 Mesenchymal Stem Cell Growth Supplement-animal component free 5 ml
MCDS 間充質干細胞軟骨分化生長因子 Mesenchymal Stem Cell Chondrogenic Differentiation Supplement 5 ml
MEpiCGS 乳腺上皮細胞生長因子 Mammary Epithelial Cell Growth Supplement 5 ml
FBS 胎牛血清 Fetal Bovine Serum, Human Cell Tested 10 ml
FBS 胎牛血清 Fetal Bovine Serum, Human Cell Tested 25 ml
T/E 胰酶/EDTA消化液 Trypsin/EDTA solution 100 ml
TNS 胰酶中和液 Trypsin Neutralization Solution 100 ml
TNS-sf 胰酶中和液-無血清 TNS-sf (Trypsin Neutralization Solution-serum free) 100 ml
ECDS 非酶細胞裂解液 Enzyme-Free Cell Dissociation Solution 100 ml
CFM 細胞凍存液 Cell Freezing Medium 50 ml 【人淀粉樣前體蛋白(βAPP)ELISA試劑盒廠家】
SF-CFM 無血清細胞凍存液 Serum-Free Cell Freezing Medium 50 ml
StemCryo 人胚胎干細胞凍存液 Human Pluripotent Stem Cell Cryopreservation Medium 50ml
StemCryo 人胚胎干細胞凍存液 Human Pluripotent Stem Cell Cryopreservation Medium, 5 x 10mL
TB 臺盼藍 Trypan Blue, 0.4% 50 ml
DPBS 磷酸鹽緩沖液 Dulbecco’s Phosphate-Buffered Saline 500 ml
HBSS 平衡鹽溶液 Hank＇s Balanced Salt Solution 500 ml
PLL 多聚賴氨酸 Poly-L-Lysine 1 mg/ml 1 ml
PLL 多聚賴氨酸 Poly-L-Lysine 10 mg/ml 1 ml
GSN 0.2％的明膠溶液 0.2% Gelatin Solution 100ml
FBS 胎牛血清 Fetal Bovine Serum, Human Cell Tested 500 ml
P/S 青霉素/鏈霉素溶液 Penicillin/Streptomycin Solution 5 ml
P/S 青霉素/鏈霉素溶液 Penicillin/Streptomycin Solution 100 ml
AMS 抗真菌溶液 Antimycotic Solution 50 ml
ABAMS 抗菌/抗霉菌溶液 Antibiotic/Antimycotic Solution 50 ml
