齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1。我必須運行所有的標準和樣品的重復？

是的，重復的運行，以監測測定的精度和提高檢測結果的信心。

2。我必須在一次運行所有的樣品嗎？

不，每個試劑盒采用stripwell微孔板。這允許用戶在不同的時間來分析不同數量的樣品。

3。什么類型的可重復性的結果，得到與分析？

每一個工具都有一個包含一個典型數據的圖表的手冊。在運營商的任何變化，移液和洗滌技術，培養時間、溫度、試劑盒的年齡可以引起變化的結果。每個用戶都應該得到自己的標準曲線。

4。是否有可能儲存的試劑比表示？

在不推薦使用條件下的部件組件的存儲，以保證測試的正確性能。

5。我應該如何儲存我的樣品？

樣品應保存在-20°C或更低的溫度。長期儲存，建議凍結他們在- 70余。

6。我可以修改協議嗎？

BG的ELISA試劑盒進行了優化，提供*佳的結果。修改的格式或協議可能會給出錯誤的結果。

7。我可以使用一個不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗證在試劑盒插入的樣品類型。未經檢驗的樣品類型。為進一步信息的聯系技術服務。

8。我的樣本產生的值，在動態范圍內的測定。我可以使用這些值嗎？

建議只有在標準曲線的范圍內下降的樣本值。標準曲線的范圍內的值一般是非線性的，這可能導致不正確的外推值。產生值高于標準的樣品應為（進一步）稀釋和重復。如果樣品低于該測定的范圍內，該樣品被認為是不可檢測的。

9。我每次都要運行一個空白或零標準嗎？

是的，這些都是必需的計算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測到檢測。當排除特定的檢測問題的來源時，它們也非常有幫助。

10。我能改變我在檢測中使用的樣本量嗎？

不建議你改變卷由于BG工具被設計為*佳的性能在給定的體積

11�？梢允褂貌煌慕M件組件嗎？

每一個組件包含有特定批號的組件，以確保所有的組件都進行優化，以及與組件中的所有其他組件。對這些特定的地段進行質量檢驗。這是從來沒有建議使用你自己的組件或組件從其他包或供應商。

12。我的標準曲線看起來很好，但我沒有得到一個信號，我的樣本，當我預期，為什么？

樣品不包含被分析物。一個矩陣的效果可能是掩蓋檢測。確保推薦的稀釋液在試劑盒插入后按規定進行。如果建議稀釋，請檢查是否正確進行稀釋。過稀釋會導致樣品在標準曲線的范圍內下降。

13。你怎么推薦我洗盤子？

如果您使用的是一個自動平板洗衣機，我們建議定期檢查校準，并將該系統在洗滌前沖洗緩沖液沖洗。手動洗衣機也是一樣的。一個中繼器或洗瓶也可以使用。用戶應小心，以確保所有的內容都是送氣和板拍干的無塵紙。【人主要組織相容性復合體Ⅱ類(MHCⅡ/HLA-Ⅱ)ELISA試劑盒現貨】

14。我需要用一個盤子嗎？

可靠的結果可以得到無板振動，但外徑的一般會低于那些使用平板振動器。

15。為什么我要用波長校正450-570nm之間？

對于酶聯免疫吸附試驗，在雙波長讀的是做正確的光密度貢獻的塑料以及，燈和光學波動。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預期的分析物濃度，我應該找到？

一個給定的分析物的量可能會有所不同，不僅從物種的物種，但也組織和細胞來源。這個信息的*佳來源是目前的文獻，很容易通過互聯網在多個科學數據庫訪問。

18。我的光密度有點高（或較低），比那些在手冊中，與我的試劑盒來了。為什么？

光學密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【人主要組織相容性復合體Ⅱ類(MHCⅡ/HLA-Ⅱ)ELISA試劑盒現貨】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【人主要組織相容性復合體Ⅱ類(MHCⅡ/HLA-Ⅱ)ELISA試劑盒現貨】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【人主要組織相容性復合體Ⅱ類(MHCⅡ/HLA-Ⅱ)ELISA試劑盒現貨】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

673113     ipsogen JAK2 MutaScreen RS Kit (19)                CNY                             

673223     ipsogen JAK2 MutaScreen EZ Kit (24) CE          CNY                             

672691     ipsogen RARA-PML bcr3 Standards          CNY                             

670115     ipsogen BCR-ABL1 Mbcr Kit (52)               CNY                             

380317     Investigator Triplex AFSQS Kit (400)                  CNY                             

9001527  PyroMark Q96 MD Automated        For performing automated pyrosequncing reaction on the Q96 MD      CNY                            

9001301  QIAsymphony AS               CNY                             

4883004  pg AIV-H5 RT-PCR ASSAY LE               CNY                              【人主要組織相容性復合體Ⅱ類(MHCⅡ/HLA-Ⅱ)ELISA試劑盒現貨】

4826024  careHIV-1 RT-PCR ASSAY V2              CNY                             

4843014  pg HPV PCR ASSAY            CNY                             

130901     NeXtal DWBlock Classics Suite                   CNY                             

130724     NeXtal Tubes JCSG Core I Suite                  CNY                             

9013221  Holder, tube, STS, single, 5 ml (60), 0                CNY                             

9013206  Fuse set, BR8000               CNY                             

132630     NeXtal Stock Zinc acetate (50)         1M Zinc acetate dihydrate - 50mL (4 x 12,5 ml tube)     CNY                             

765112     PAXgene Tissue Container (10), G             CNY                             

762331     PAXgene 96 Blood RNA Kit (4) 4 PAXgene 96 RNA Plates, 4 PAXgene 96 Filter Plates, Buffers, Proteinase K, RNase-free DNase Sets, AirPore Tape Sheets, Collection Vessels. To be used in conjunction with PAXgene Blood RNA Tubes.          CNY                            

761133     PAXgene Blood DNA Kit (25)    Processing tubes ａnd buffers for 25 preparations   CNY                             

203607     HotStarTaq Plus DNA Polymerase (5000)         1 x 5000 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2        CNY                             

203203     HotStarTaq DNA Polymerase (250) - 250 units HotStarTaq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2         CNY                             

200403     TopTaq Master Mix Kit (250)   2x TopTaq Master Mix containing 250 units of TopTaq DNA Polymerase in total, 10x CoralLoad Concentrate, ａnd RNase-Free Water; reagents provided for 200 x 50 μl reactions        CNY                             

159994     Generation DNA Elution Soln 2 (500 ml)           CNY                             

158932     Clotspin Baskets (50)                  CNY                             

158867     Gentra Puregene Buccal Cell Kit (400)              CNY                              【人主要組織相容性復合體Ⅱ類(MHCⅡ/HLA-Ⅱ)ELISA試劑盒現貨】

158467     Gentra Puregene Blood Kit (120 ml)                 CNY                             

34964       Polypropylene Columns (5-ml) 50/pack, 5 ml capacity     CNY                             

34530       Ni-NTA HRP Conjugate     Horseradish-peroxidase-conjugated Ni-NTA (lyophilized, for 500 ml working solution)  CNY                             

34342       TAGZyme Qcyclase/pGAPase Enz. (150U/50U)        For processing of approximately 50 mg tagged protein: 150 units Qcyclase Enzyme, 50 units pGAPase Enzyme  CNY                             

34210       E. coli Host Strains   One stab culture each: E. coli M15[pREP4],  SG13009[pREP4]     CNY                             

32942       His Strep pQE-TriSystem Vector Set          pQE-TriSystem His-Strep 1 ａnd pQE-TriSystem His-Strep 2 vectors, 25 ug each         CNY                             

32713       EasyXpress pIX4.0 Vector                   CNY                             

NG0122   尿糖試劑         100毫升 班氏法                                 【人主要組織相容性復合體Ⅱ類(MHCⅡ/HLA-Ⅱ)ELISA試劑盒現貨】

NG0123   尿糞隱血測試盒     100T         聯苯胺法                                    

NG0124   堿性磷酸酶染液/cAKPstain   1套 可染20-30片                            

NG0125   酸性磷酸酶染液/cACPstain   1套 可染20-30片                            

NG0126   酸性酯酶染液/Esterasestain 5毫升×4        染色                                    

NG0127   中性酯酶染液/Esterasestain 5毫升×4        染色                                    

NG0128   堿性酯酶染液/Esterasestain 5毫升×4        染色                                    

NG0129   雙重酯酶染液/Esterasestain 5毫升×4        染色                                    

NG0131   糖元染液/糖原染液/PASStain         10毫升    染色                                    

NG0134   H-E染液/蘇木素-伊紅染液/Hematoxylin-Eosinstain     25毫升×5      BR                               

NG0135   瑞氏姬姆薩復合染液/瑞氏吉姆薩復合染液/Wright-Giemsastain      90毫升    BR                               

NG0136   快速改良巴氏染液/Fastmodifiedpapanicolaoustain      50毫升×2；100毫升×1     BR                               

NG0137   改良巴氏染液/Modifiedpapanicolaoustain    50毫升×5；100毫升×1     BR                               

NG0138   植物樣本提取液     100毫升 BR                               

NG0139   抗酸染液/Acid-faststain 50毫升×3      BR                               

NG0140   果糖測試盒     50管/48樣      比色法                                

NG0141   微量丙二醛測試盒/MDA測試盒  50管/24樣      比色法                                 【人主要組織相容性復合體Ⅱ類(MHCⅡ/HLA-Ⅱ)ELISA試劑盒現貨】

NG0142   細胞活性鑒別染液/Cellviabilitydetectedstain       20毫升    BR                               

NG0143   血管通透性測試染液/Vasopermeabilitystain        20毫升    BR                               

NG0144   過氧化物酶染液/Peroxidasestain  5毫升×3；2毫升×1   BR                               

NG0145   血清高密度脂蛋白膽固醇測試盒/HDL-C測試盒         80毫升    沉淀分離法手工                                

NG0146   血清低密度脂蛋白測試盒/LDL測試盒         80毫升    手工/半自動                              

NG0147   蔗糖測試盒     50管/48樣      比色法                                

NG0148   蔗糖磷酸合成酶測試盒/SPS測試盒     50管/24樣      比色法                                

NG0149   蔗糖合成酶測試盒/SS測試盒       50管/24樣      比色法                                

NG0150   硝酸還原酶測試盒/NR測試盒      50管/24樣      比色法                                

NG0151   ATP含量測試盒      100管/48樣    比色法                                

NG0152   γ-谷氨酰胺半胱氨酸合成酶測試盒/γ-GCS測試盒 100管/48樣    微量定磷法                                

NG0153   F0F1-ATP酶/ATP合成酶測試盒     20管/10樣      生化法                                

NG0154   二胺氧化酶測試盒/DAO測試盒   25毫升/40T     手工比色法                                

NG0155   質粒提取試劑盒     100T/套   BR                               

NG0156   紅細胞裂解液         100毫升 BR                               

NG0157   RIPA組織細胞快速裂解液    20毫升    BR                                【人主要組織相容性復合體Ⅱ類(MHCⅡ/HLA-Ⅱ)ELISA試劑盒現貨】