1.購買我公司任意ELISA試劑盒指標享受免費代測，并提供技術指導服務！

二、實驗材料與試劑配制：

1. 儀器與材料：酶標儀（使用前預熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時備用（如果標本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人硫酸肝素糖蛋白(HSPG)ELISA試劑盒廠家】三. 仔細閱讀說明書

1.檢查試劑盒標簽上的有效期。如果超過有效期，請勿使用。

2. 按說明書確定所有試劑齊全（數量、體積）

3. 標本制備要規范，每份標本體積要按2-3個復孔以上的量制備（貯存），盡量分裝做備份。

4.短期內無法實驗者，注意低溫保存

5.準備好所有實驗額外所需物品，（比如移液器，試管，清洗器，酶標儀）

6. 按說明書將所用試劑平衡至室溫，根據檢測標本數量確定所需試劑的量

7.檢查試劑盒內每種試劑的貯存條件，保證所有試劑均按照試劑盒內說明書推薦的條件存儲。 【人硫酸肝素糖蛋白(HSPG)ELISA試劑盒廠家】

8.檢查不穩定或者變質的試劑溶液（比如沉淀或變色）。有些試劑，比如標準品稀釋液或者檢測稀釋液，可能在設計時就含有沉淀物。所有試劑在滴入酶標板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標板之前，必需不停攪拌。

9.保證充分的孵育時間和溫度。

10.切勿使用不同生產批號的試劑取代現有試劑或混合現有試劑。

11.在混合或溶解蛋白溶液時，避免泡沫產生。

12.在實驗開始之前，安排好實驗流程。在實驗開始之前，清潔工作臺。

13.若有問題，應與我公司或代理商的技術支持聯系

【人硫酸肝素糖蛋白(HSPG)ELISA試劑盒廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩定。我們提倡新鮮標本盡早檢測，對收集后當天就進行檢測的標本，及時儲存在4℃備用，如有特殊原因需要周期收集標本，請造模取材后，將標本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質量，所以避免反復凍融。部分標本也可加入適當防腐劑，激素類標本需添加抑肽酶。 

2.試劑盒代測標本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項請與我司技術人員溝通。

3.液體類標本：標本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細胞培養上清、組織勻漿等。

4.購買前請提前告訴我們：您樣本的種屬、樣本數量、待測指標及其它特殊要求。

【人硫酸肝素糖蛋白(HSPG)ELISA試劑盒廠家】五、代測數據詳細內容

1、ELISA標準曲線；

2、實驗具體詳細步驟；

3、完整的實驗報告（試劑耗材，實驗方法，實驗結果及結果說明）；

4、實驗進行階段，我公司客服人員會及時向您匯報實驗進程。

9020319  PM kit, C12 & QIAgility (UV), CG1             CNY                             

9017625  Motor, y-drive (24V), BR8RD            CNY                             

9020254  LED, LightStrip (white), Qsym           CNY                             

9017354  Holder, VacTrap, BR8                CNY                             

9020201  PCB, lrge breakout, 12 core to rev8, CC2                  CNY                             

9020199  PCB, Z plate breakout,8 ch., 8 core, QX1                  CNY                             

9020193  PCB, vac pump, QXT                  CNY                             

9239523  Fitting,1/16MNPT,1/8, forWasteBottle(5)                  CNY                             

9239536  Assy,Terminated,RedLED,SDS (2)              CNY                             

9239529  Fuse&Holder,1Amp,Fblo,PicoSmd(5)                 CNY                             

9020159  Mask, protection - chamber bottom, RG3                CNY                             

9020145  QIAplex MDD HPV Genotyping LQ Software            CNY                             

9020114  USB cable, QGX                 CNY                             

9020102  Packaging, TLyser2           CNY                              【人硫酸肝素糖蛋白(HSPG)ELISA試劑盒廠家】

9020100  Shipping Lock Screw, TLyser 2           CNY                             

9020094  Left Ratchet Holder, TLyser 2           CNY                             

9020090  Motor 24V DC, TLyser 2            CNY                             

9239518  Tubing. Peek, 1/6OD, 010ID, 10 (qty 5)             CNY                             

9239492  Hyd Arm Optic Switch               CNY                             

9239449  3A, 250V Fast Acting Fuse                 CNY                             

9232867  Sensor, pressure, 1600-1mbar                   CNY                             

9236775  Sealing ring, auto vac, SBS top, BR3000           CNY                             

9236509  BR 8000, PM Service        The Overhaul covers all travel ａnd labor costs plus all necessary parts to perform a Preventative Maintenance,. The instrument will also be thoroughly checked ａnd inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed.     CNY                             

9239360  Z-Axis Coupling, EZ1                   CNY                             

9236478  BR 9604, Full Agreement         The Full Agreement 9604, 1 PM provides full service coverage for the BioRobot 9604. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).         CNY                             

9236476  #Agreement, 9604, 2PM, 1 yr        An agreement for preventive maintenance provides coverage for travel, labor, ａnd parts, plus the cost of consumables required for two maintenance visits during 1 year. Work carried out during the preventive maintenance visit is fully documented in accordance with GMP/GLP directives. CNY                             

9232726  Block, 2-way, v1.02           CNY                             

9239540  Cable Assy Extender, 2 wire, 22AWG, 12in                CNY                             

9239396  Upgr. LiquiChip Analyzer (RUO)                 CNY                             

9239496  EX XY Motor Driver w/Encoder                 CNY                              【人硫酸肝素糖蛋白(HSPG)ELISA試劑盒廠家】

9239494  Sheath Fluid Tubing w/DeBubbler             CNY                             

QY-x638   PC-3前列腺癌細胞                                                       

QY-x639   BEL-7402肝細胞癌細胞                                                       

QY-x640   Tsu-Pr1非雄激素依賴型前列腺癌                                                      

QY-x641   HCC-9204肝癌細胞                                                      

QY-x642   HOS骨肉瘤細胞                                                   

QY-x643   Hep3B肝癌細胞                                                   

QY-x644   MG-63成骨肉瘤細胞                                                  

QY-x645   Hep G2肝癌細胞                                                 

QY-x646   Saos-2骨肉瘤細胞                                               

QY-x647   SMMC-7721肝癌細胞                                                   【人硫酸肝素糖蛋白(HSPG)ELISA試劑盒廠家】

QY-x648   U-2 OS骨肉瘤細胞                                                       

QY-x649   PAN-1胰腺導管上皮癌細胞                                               

QY-x650   OS-732骨肉瘤細胞（瘤株）                                                       

QY-x651   Panc 10.05胰腺腺癌細胞                                                    

QY-x652   A-204橫紋肌肉瘤細胞                                                

QY-x653   SW1990胰腺腺癌細胞                                                

QY-x654   HT1080纖維肉瘤細胞                                                 

QY-x655   SK-RC-42腎癌細胞                                               

QY-x656   A375皮膚黑色素瘤細胞                                                      

QY-x657   RCC-krause腎癌細胞                                                    

QY-x658   A875黑色素瘤細胞                                                      

QY-x659   Ketr-3腎癌（瘤株）                                                    

QY-x660   SK-HEL-1皮膚黑色素瘤細胞                                               

QY-x661   SW 13腎上腺皮質瘤細胞                                                    

QY-x662   A431皮膚基底細胞癌細胞                                                 

QY-x663   96-C低轉移肺癌細胞                                                   【人硫酸肝素糖蛋白(HSPG)ELISA試劑盒廠家】

QY-x664   CEM白血病細胞                                                  

QY-x665   95-D高轉移肺癌細胞                                                  

QY-x666   K562慢性髓原白血病細胞                                                 

QY-x667   973肺腺癌細胞                                                    

QY-x668   HPB-ALLT細胞白血病細胞                                                  

QY-x669   A2肺腺癌細胞                                                      

QY-x670   HL-60白血病細胞