1.購買我公司任意ELISA試劑盒指標享受免費代測，并提供技術指導服務！

二、實驗材料與試劑配制：

1. 儀器與材料：酶標儀（使用前預熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時備用（如果標本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人抗軟骨抗體(anti-cartilage-Ab)ELISA試劑盒廠家】三. 仔細閱讀說明書

1.檢查試劑盒標簽上的有效期。如果超過有效期，請勿使用。

2. 按說明書確定所有試劑齊全（數量、體積）

3. 標本制備要規范，每份標本體積要按2-3個復孔以上的量制備（貯存），盡量分裝做備份。

4.短期內無法實驗者，注意低溫保存

5.準備好所有實驗額外所需物品，（比如移液器，試管，清洗器，酶標儀）

6. 按說明書將所用試劑平衡至室溫，根據檢測標本數量確定所需試劑的量

7.檢查試劑盒內每種試劑的貯存條件，保證所有試劑均按照試劑盒內說明書推薦的條件存儲。 【人抗軟骨抗體(anti-cartilage-Ab)ELISA試劑盒廠家】

8.檢查不穩定或者變質的試劑溶液（比如沉淀或變色）。有些試劑，比如標準品稀釋液或者檢測稀釋液，可能在設計時就含有沉淀物。所有試劑在滴入酶標板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標板之前，必需不停攪拌。

9.保證充分的孵育時間和溫度。

10.切勿使用不同生產批號的試劑取代現有試劑或混合現有試劑。

11.在混合或溶解蛋白溶液時，避免泡沫產生。

12.在實驗開始之前，安排好實驗流程。在實驗開始之前，清潔工作臺。

13.若有問題，應與我公司或代理商的技術支持聯系

【人抗軟骨抗體(anti-cartilage-Ab)ELISA試劑盒廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩定。我們提倡新鮮標本盡早檢測，對收集后當天就進行檢測的標本，及時儲存在4℃備用，如有特殊原因需要周期收集標本，請造模取材后，將標本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質量，所以避免反復凍融。部分標本也可加入適當防腐劑，激素類標本需添加抑肽酶。 

2.試劑盒代測標本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項請與我司技術人員溝通。

3.液體類標本：標本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細胞培養上清、組織勻漿等。

4.購買前請提前告訴我們：您樣本的種屬、樣本數量、待測指標及其它特殊要求。

【人抗軟骨抗體(anti-cartilage-Ab)ELISA試劑盒廠家】五、代測數據詳細內容

1、ELISA標準曲線；

2、實驗具體詳細步驟；

3、完整的實驗報告（試劑耗材，實驗方法，實驗結果及結果說明）；

4、實驗進行階段，我公司客服人員會及時向您匯報實驗進程。

QY-03220 AEBP1 (Adipocyte enhance binding protein 1)脂肪細胞增強結合蛋白1                0.5mg/6501mg/1100                                  

QY-03970 MMP-9(matrix metalloproteinase 9)基質金屬蛋白酶-9(抗原)                0.5mg/6501mg/1100                                   【人抗軟骨抗體(anti-cartilage-Ab)ELISA試劑盒廠家】

QY-01770 AGER（Advanced glycosylation end product-specific receptor）晚期糖基化終末產物特異性受體(抗原）               0.5mg/6501mg/1100                                  

QY-02370 14-3-3 protein14-3-3 蛋白（多肽抗原）                0.5mg/6501mg/1100                                  

QY-00370 AIF�。�Apoptosis-Inducing Factor）調亡誘導因子(多肽片斷抗原）                    0.5mg/6501mg/1100                                  

QY-02990 Adenylate Kinase 1 (AK-1)腺苷酸激酶-1                   0.5mg/6501mg/1100                                  

QY-00370 AIF�。�Apoptosis-Inducing Factor）調亡誘導因子(多肽片斷抗原）                    0.5mg/6501mg/1100                                  

QY-01150 Akt/PKB蛋白激酶B（抗原）                  0.5mg/7001mg/1200                                 

QY-08610 2,4-D/KLH（2,4-Dichlorophenoxyacetic acid）2，4-二氯苯氧乙酸偶聯血藍蛋白               5mg/1500                                   

QY-01150 Akt/PKB蛋白激酶B（抗原）                  0.5mg/7001mg/1200                                 

QY-00970 ALK （Anaplastic Lymphoma Kinase）（CD246Ag）間變型淋巴瘤激酶（抗原）                0.5mg/6501mg/1100                                  

QY-00070 AM Adrenomedullin 腎上腺髓質素（抗原）                 0.1mg/1800                               

QY-04160 TIMP-2(Tissue Inhibitor of Metalloproteinase-2)金屬蛋白酶組織抑制因子-2(抗原)             0.5mg/6501mg/1100                                  

QY-04170 TIMP-3(Tissue Inhibitor of Metalloproteinase-3)金屬蛋白酶組織抑制因子(抗原)                 0.5mg/6501mg/1100                                  

QY-00900 ADM R, Adrenomedullin receptor腎上腺髓質素受體（抗原）            0.5mg/6501mg/1100                                  

Yf-01580  Amylin1-37/FITC異硫氰酸熒光素(FITC)標記糖尿病相關肽1-37           1ml/2860                                     

QY-03060 VTG(vitellogenin)青鳉魚卵黃蛋白                    0.1mg/6500.2mg/1100                              

QY-00580 Apaf-1 （Apoptosis protease activating factor-1）(CT)凋亡蛋白活性因子-1（抗原）                0.5mg/7001mg/1200                                  【人抗軟骨抗體(anti-cartilage-Ab)ELISA試劑盒廠家】

QY-00590 Apaf-1 （Apoptosis protease activating factor-1）(NT)凋亡蛋白活性因子-1（抗原）               0.5mg/7001mg/1200                                 

QY-01180 AR （Androgen Receptor)雄激素受體（抗原）           0.5mg/6501mg/1100                                  

QY-00450 AT （Angiotenogen）血管緊張素原（抗原）              0.5mg/6501mg/1100                                  

QY-01670 Apo-E （Apolipoprotein E）載脂蛋白E（抗原）                   0.5mg/6501mg/1100                                  

QY-02450 IKI3 family protein             0.5mg/8001mg/1200                                  

QY-03170 ILK-1 (Integrin-linked protein kinase 1)整合素連接激酶-1               0.5mg/6501mg/1100                                  

QY-01140 Aromatase芳香化酶（抗原）                 0.5mg/6501mg/1100                                  

QY-00550 ART(Artemin)（抗原）             0.5mg/6501mg/1100                                  

QY-02610 AQP-2 （aquaporin Protein-2）水通道蛋白-2（抗原）                0.5mg/6501mg/1100                                  

QY-01640 BACE( ASP2 ） β分泌酶（抗原）                 0.5mg/6501mg/1100                                  

QY-02750 Bassoon(RCN)            0.5mg/6501mg/1200                                  

QY-01270 Bax （Bcl-2 Assaciated X protein） Bax（多肽抗原）                  0.5mg/6501mg/1100                                  

QY-00320 Bcl-2（抗原）Bcl-2（多肽抗原）                    0.5mg/6501mg/1100                                  

QY-02480 BDNF (Brain-derived Neurotrophin factor)腦源神經營養因子(腦衍化神經營養因子)                  0.5mg/6501mg/1100                                  

QY-02170 bFGF堿性成纖維細胞生長因子（多肽抗原）              0.5mg/6501mg/1100                                  

9018902  Loading Block 72 x 0.1 mL Multi-channel                  CNY                             

9018558  Door, w/o hood ａnd reflector, EZ1a                  CNY                             

9013112  Vacuum Manifold 2.03              CNY                             

670291     ipsogen BCR-ABL1 mbcr Standards          CNY                             

387016     Investigator Quantiplex Kit (200)              CNY                              【人抗軟骨抗體(anti-cartilage-Ab)ELISA試劑盒廠家】

380327     Investigator Triplex DSF Kit (400)              CNY                             

9001492  EZ1 Advanced XL               CNY                             

9001230  LiquiChip Workstation IS 200             CNY                             

9000852  BioSprint 96               CNY                             

4880004  PG Influenza A RT-PCR Kit (48) LE Z                   CNY                             

130908     NeXtal DWBlock Cations Suite                   CNY                             

4559265  artus C.trachomatisPlus RG PCR Kit(96)CE                CNY                             

4801014  careHBV PCR ASSAY                   CNY                             

4803004  careHBV Mutation PCR ASSAY                   CNY                             

9240599  1Brake cylinder, Elevator, AP98                CNY                             

9240582  Filter, Regulator, AP96              CNY                             

9240559  Temp sensor, Cond., Eppendorf, AP196/98                 CNY                             

9240556  Shutter Door Assy, Eppendorf, AP196/98          CNY                             

9240538  Cable Assy, SiNug, Mtr-Control, AP96/196                 CNY                             

9240537  Controller N2, SiNug, AP96/196                 CNY                             

9240505  PC-104, AP196          CNY                              【人抗軟骨抗體(anti-cartilage-Ab)ELISA試劑盒廠家】

9240244  Cap, brushes, TR                CNY                             

9240103  Re-Installation, BR MDx DSP   The Re-Installation, BR MDx DSP facilitates quick implementation of system back into lab routine by minimizing instrument set-up phase. It covers hardware ａnd software check of instrument ａnd provides user with operator refesher overview.  Re-installation includes labor ａnd travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.          CNY                             

9240046  Thermostate unit, 220V, 1.04          CNY                             

9239556  Assy,Kit,Retrofit,LaserDriverBoardUnlim                  CNY                             

9020027  Sensor, solenoid, 170mm, EZ1a                 CNY                             

9019500  UV light system, CAS                  CNY                             

9019499  ReagBlock, 8x1.5ml/2ml/1.5ml Ta/Flat/Ta                CNY                             

9241225  QIAcube, Inspection Service    "The Annual Inspection Service, QIAcube is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist. During the half day-long procedure, all application-critical modules of the QIAcube are inspected ａnd tested. Performed tests ａnd test results are documented in a Visit Report."  CNY                             

9241218  HC2, Full Agreement, no PM   "Service response time (on-site repair):not applicable

Periodic inspection/maintenance: no, not included

Cost coverage depot repair (parts, labor, packaging, shipping): yes, full coverage

For defect DML, after customer calling QIAGEN technical service hotline before 12 am:

- A special package containing a replacement unit shall be sent to the customer within 2 working days.

- Customer shall send the defect unit to QIAGEN Regional Repair Center following the instructions attached with the packaging.  

For DML owned by customer, QIAGEN Regional Repair Center will send back the repaired original unit to customers within 7-10 working days.

PC, Monitor, Heater, Shaker, Washer, Printer ａnd Votexer:

- A new unit shall be sent to customer within  7-10 working days at no extra cost

- Customer shall send the defect unit to QIAGEN for disposal

- On-site installation of PC ａnd application software is not covered by the agreement"         CNY                             

9241182  Belt, Y, QX                  CNY