檢測標本：血清,血漿,尿液,胸腹水,腦脊液,細胞培養上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯免疫夾心法

產品的用途：僅供科研究課題使用

價格及詳細資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認同.大品牌保證,價格公道,傾力為國內外科研院校實驗室提供*優質的產品.若有需要,我司將竭誠為您服務！本試劑盒用于測定血清,血漿及相關液體樣本中含量.

【大鼠結腸組織黏蛋白-2(MUC-2)ELISA試劑盒廠家】實驗原理：

   本試劑盒應用雙抗體夾心法測定標本中該產品水平.用純化的本產品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產品抗原,再與HRP標記的本產品抗體結合,形成抗體-抗原-酶標抗體復合物,經過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉化成藍色,并在酸的作用下轉化成*終的黃色.顏色的深淺和樣品中的本產品呈正相關.用酶標儀在450nm波長下測定吸光度（OD值）,通過標準曲線計算樣品中該產品濃度.

試劑盒組成：

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【大鼠結腸組織黏蛋白-2(MUC-2)ELISA試劑盒廠家】標本要求：

1．標本采集后盡早進行提取,提取按相關文獻進行,提取后應盡快進行實驗.若不能馬上進行試驗,可將標本放于-20℃保存,但應避免反復凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標準品的稀釋與加樣：在酶標包被板上設標準品孔10孔,在、第二孔中分別加標準品100μl,然后在、第二孔中加標準品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標準品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標準品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標準品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標準品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【大鼠結腸組織黏蛋白-2(MUC-2)ELISA試劑盒廠家】

2.     加樣：分別設空白孔（空白對照孔不加樣品及酶標試劑,其余各步操作相同）、待測樣品孔.在酶標包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標板孔底部,盡量不觸及孔壁,輕輕晃動混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復5次,拍干.

6.     加酶：每孔加入酶標試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應（此時藍色立轉黃色）.

11. 測定：以空白空調零,450nm波長依序測量各孔的吸光度（OD值）. 測定應在加終止液后15分鐘以內進行.

注意事項：

1． 試劑盒從冷藏環境中取出應在室溫平衡15-30分鐘后方可使用,酶標包被板開封后如未用完,板條應裝入密封袋中保存.

2． 濃洗滌液可能會有結晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結果. 【大鼠結腸組織黏蛋白-2(MUC-2)ELISA試劑盒廠家】

3． 各步加樣均應使用加樣器,并經常校對其準確性,以避免試驗誤差.一次加樣時間控制在5分鐘內,如標本數量多,推薦使用排搶加樣.

4． 請每次測定的同時做標準曲線,做復孔.如標本中待測物質含量過高（樣本OD值大于標準品孔孔的OD值）,請先用樣品稀釋液稀釋一定倍數（n倍）后再測定,計算時請*后乘以總稀釋倍數（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請避光保存.

7． 嚴格按照說明書的操作進行,試驗結果判定必須以酶標儀讀數為準.

8． 所有樣品,洗滌液和各種廢棄物都應按傳染物處理.

9． 本試劑不同批號組分不得混用.

10. 如與英文說明書有異,以英文說明書為準.

計算：

以標準物的濃度為橫坐標,OD值為縱坐標,   

在坐標紙上繪出標準曲線,根據樣品的OD     

值由標準曲線查出相應的濃度；再乘以稀釋     

倍數；或用標準物的濃度與OD值計算出標     

準曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計算出樣品濃度,再乘以稀釋

倍數,即為樣品的實際濃度.

試劑盒性能：

1.樣品線性回歸與預期濃度相關系數R值為0.95以上.

2.批內與批見應分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個月

5123-1220        Female Swab Specimen Collection KitTM                  CNY                             

5100-1400IVD hc2 Sample Conversion Kit                CNY                             

EA11424  EASYartus BKV RG_II                 CNY                             

5050-1075 V.1 CD Rom Manual, MST Vortexer 2             CNY                             

EA10124  EASYartus EBV RG PCR Kit 2             CNY                             

BR-02       DxS B-RAF Mutation Test Kit            CNY                             

9240729  Tubing, 3/8"OD , Red, AP          CNY                             

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9240696  Fitting, Barbed, 1/4" ID tube, AP               CNY                             

9243651  Comprehen Valid., QIAgility     """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   For planning purposes ａnd to ensure a smooth process, the Customer is expected to have all materials on-hand ａnd inventoried one (1) week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """     CNY                             

218076     miScript SYBR Green PCR Kit (2000)                  CNY                             

271906     cattletype Milk Prep Kit (50)             CNY                             

289980     intype IC-DNA (1ml)                   CNY                              【大鼠結腸組織黏蛋白-2(MUC-2)ELISA試劑盒廠家】

280437     virotype BTV RT-PCR Kit (480)          CNY                             

932836     #QIAsymphony Ni-NTA Denat. Kit (384)           CNY                             

800511     Verification Sequencing / DNA production                CNY                             

9238243  ZLV BioRobot M96 User Manual               CNY                             

9019988  ZLV Mainboard, QGY                 CNY                             

9020553  Foam angle, transport box, QIAC              CNY                             

9020582  X-Motor, DML3000           CNY                             

9020584  Y-Belt, DML3000 320 Teeth              CNY                             

9020594  Enclosure, DML3000                 CNY                             

9021006  Motor, Pipettor Y, DCU             CNY                             

9236333  Sample Loop              CNY                             

9013382  Shaker adapter, microplate, LHS              CNY                             

9013275  Motor, gripper, handler T/L, BR3000                CNY                             

9013269  Fitting, PA tubing M6x1, 2.25mm, (10)            CNY                             

9013210  Cabling kit, moving, BR8000             CNY                             

9013198  Tubing,pres.sen.con.auto vac,BR8000v1.00            CNY                              【大鼠結腸組織黏蛋白-2(MUC-2)ELISA試劑盒廠家】

9013188  Valve set, pressure relaese, dispenser,             CNY                             

QYS-03248        紅霉素-N-脫甲基酶ERND測試盒         100管/48樣    酶標法                                

QYS-03249        細胞色素b5含量測試盒       50管/48樣      可見分光光度法                                

QYS-03250        細胞色素b5含量測試盒       100管/96樣    酶標法                                

QYS-03251        血鉀濃度測試盒     50管/48樣      可見分光光度法                                

QYS-03252        血鉀濃度測試盒     100管/96樣    酶標法                                

QYS-03253        血鈣濃度測試盒     50管/48樣      可見分光光度法                                

QYS-03254        血鈣濃度測試盒     100管/96樣    酶標法                                

QYS-03255        血清鐵濃度測試盒         50管/48樣      可見分光光度法                                

QYS-03256        血清鐵濃度測試盒         100管/96樣    酶標法                                

QYS-03257        血鎂濃度測試盒     50管/48樣      可見分光光度法                                 【大鼠結腸組織黏蛋白-2(MUC-2)ELISA試劑盒廠家】

QYS-03258        血鎂濃度測試盒     100管/96樣    酶標法                                

QYS-03259        血磷濃度測試盒     50管48樣      可見分光光度法                                

QYS-03260        血磷濃度測試盒     100管/96樣    酶標法                                

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QYS-03262        血鈉濃度測試盒     100管/96樣    酶標法                                

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QYS-03265        血鋅濃度測試盒     100管/96樣    酶標法                                

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QYS-03274        土壤總磷/有機磷/無機磷含量測試盒 50管/24樣      可見分光光度法                                

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