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大鼠骨成型蛋白受體Ⅱ(BMPR-Ⅱ)ELISA試劑盒廠家
點擊次數:20發布時間:2016/8/5 22:17:55

更新日期:2016/8/5 22:17:55
所 在 地:中國大陸
產品型號:
優質供應
詳細內容
【友情提示】:本產品僅供科研研究使用,不得用于人體臨床直接檢測。避免給您帶來不必要的損失,請仔細閱讀購買說明!
【大鼠骨成型蛋白受體Ⅱ(BMPR-Ⅱ)ELISA試劑盒廠家】酶法檢測試驗所需自備物品:
1. 酶標儀(450nm 波長濾光片)
2. 高精度移液器,EP 管及一次性吸頭
3. 37℃恒溫箱,雙蒸水或去離子水
4. 吸水紙
試劑盒安全性提示:
1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請盡快用水沖洗。
2.實驗中不要吃喝、抽煙或使用化妝品。
3.不要用嘴吸取試劑盒里的任何成份。
【大鼠骨成型蛋白受體Ⅱ(BMPR-Ⅱ)ELISA試劑盒廠家】操作注意事項:
1.試劑盒應按標簽說明書儲存,使用前恢復到室溫30分鐘后方可使用。稀稀過后的標準品應丟棄,不可保存。
2.實驗中不用的板條應立即放回包裝袋中,密封保存,以免變質。
3.不用的其它試劑應包裝好或蓋好。不同批號的試劑不要混用.請在試劑盒保質前使用。
4.使用一次性的吸頭以免交叉污染,吸取終止液和底物A、B液時,避免使用帶金屬部分的加樣器。
5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。
6.洗滌酶標板時應充分拍干,不要將吸水紙直接放入酶標反應孔中吸水。
7.底物A應揮發,避免長時間打開蓋子。底物B對光敏感,避免長時間暴露于光下。避免用手接觸,有毒。實驗完成后應立即讀取OD值。
8.加入試劑的順序應一致,以保證所有反應板孔溫育的時間一樣。
9.按照說明書中標明的時間、加液的量及順序進行溫育操作。
【大鼠骨成型蛋白受體Ⅱ(BMPR-Ⅱ)ELISA試劑盒廠家】齊一生物客戶寄標本時需注明以下情況:
1、標本編號;2、所測項目;3、是否做復孔;3、聯系方式;4、實驗后標本是否寄回。
客戶必知:
客戶應對所提供的材料及信息負責,如因客戶提供的材料及信息不準確而引起的實驗延誤或經濟損失由客戶承擔。
齊一生物供應ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據。為了保證實驗數據的可靠性,在實驗過程中必須堅持全面質量控制和全過程質量控制。在收集標本前都必須有一個完整的計劃。
樣本保存條件:
1.2-8℃冰箱*多放2周
2.-20到-50℃的冰箱可以放3-6個月
3.-50℃以下的冰箱,可以放一年
樣本收集
1.血清樣本用干燥管(紅色)或者促凝管(黃色)收集
2.血漿用抗凝管,一般有EDTA抗凝(紫色)、枸櫞酸鈉抗凝(黑色或者藍色)
3.動物組織勻漿(細胞):
1g組織加入9ml PBS(濃度為0.1M,pH為7.4的)測分泌型指標
1g組織加入1ml組織裂解液加入8ml PBS 測細胞內
糞便樣本:1g樣本+9ml PBS(濃度為0.01M,pH為7.4的)
沒有PBS的話也可以用一下生理鹽水,還是用PBS
樣本離心轉速及時間
1.試管采集的樣本:3000轉,離心五分鐘取上清
2.離心管或者EP管采集的樣本:5000--8000轉3分鐘取上清
3..樣本保存--如果樣品不立即使用,應將其分成小部分-70 ℃保存,避免反復冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒,檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應在室溫下解凍并確保樣品均勻地充分解凍。
【大鼠骨成型蛋白受體Ⅱ(BMPR-Ⅱ)ELISA試劑盒廠家】齊一生物供應,以上資料供參考如有疑問需要詳細資料請聯系我們客服人員,齊一生物銷售:021-6034 8496;181214 53965;173021 04490
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