齊一生物試劑盒組分經過重重優化，每個組分的穩定性都完美的經過高溫破壞測驗，程度的避免了長途運輸和保存過程中抗原抗體的失活.齊一生物試劑盒憑借其完美的標曲.嚴格的質控.穩定高、易保存，操作簡便，已廣泛應用在全國免疫學檢驗研究所的各領域中.深受研究者們的青睞. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【大鼠白介素4(IL-4)ELISA試劑盒廠家】齊一生物試劑盒憑借其完美的標曲、嚴格的質控、較高的穩定性，在世界范圍內的科研試劑領域中脫穎而出深受研究者們的青睞. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【友情提示】：本產品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

試驗所需自備物品:

1. 酶標儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【大鼠白介素4(IL-4)ELISA試劑盒廠家】試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

操作注意事項：

1.試劑盒應按標簽說明書儲存，使用前恢復到室溫30分鐘后方可使用。稀稀過后的標準品應丟棄，不可保存。

2.實驗中不用的板條應立即放回包裝袋中，密封保存，以免變質。

3.不用的其它試劑應包裝好或蓋好。不同批號的試劑不要混用.請在試劑盒保質前使用。

4.使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時，避免使用帶金屬部分的加樣器。

5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。

6.洗滌酶標板時應充分拍干，不要將吸水紙直接放入酶標反應孔中吸水。

7.底物A應揮發，避免長時間打開蓋子。底物B對光敏感，避免長時間暴露于光下。避免用手接觸，有毒。實驗完成后應立即讀取OD值。

8.加入試劑的順序應一致，以保證所有反應板孔溫育的時間一樣。

9.按照說明書中標明的時間、加液的量及順序進行溫育操作。

【大鼠白介素4(IL-4)ELISA試劑盒廠家】齊一生物供應ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據。為了保證實驗數據的可靠性，在實驗過程中必須堅持全面質量控制和全過程質量控制。在收集標本前都必須有一個完整的計劃。

操作步驟
實驗開始前，請提前配置好所有試劑，試劑或樣品稀釋時，均需混勻，混勻時盡量避免起泡。每次檢測都應該做標準曲線。如樣品濃度過高時，用樣品稀釋液進行稀釋，以使樣品符合試劑盒的檢測范圍。
1.         加樣：分別設空白孔、標準孔、待測樣品孔�？瞻卓准訕悠废♂屢�100μl，余孔分別加標準品或待測樣品100μl，注意不要有氣泡，加樣將樣品加于酶標板孔底部，盡量不觸及孔壁，輕輕晃動混勻，酶標板加上蓋或覆膜，37℃反應120分鐘。
為保證實驗結果有效性，每次實驗請使用新的標準品溶液。
2.         棄去液體，甩干，不用洗滌。每孔加生物素標記抗體工作液 100μl（取1μl生物素標記抗體加99μl生物素標記抗體稀釋液的比例配制，輕輕混勻，在使用前一小時內配制），37℃,60分鐘。
3.         溫育60分鐘后，棄去孔內液體，甩干，洗板3次，每次浸泡1-2分鐘，350μl/每孔，甩干。
4.         每孔加辣根過氧化物酶標記親和素工作液（同生物素標記抗體工作液） 100μl，37℃，60分鐘。
5.         溫育60分鐘后，棄去孔內液體，甩干，洗板5次，每次浸泡1-2分鐘，350μl/每孔，甩干。
6.         依序每孔加底物溶液90μl，37℃避光顯色（30分鐘內，此時肉眼可見標準品的前3-4孔有明顯的梯度藍色，后3-4孔梯度不明顯，即可終止）。
7.         依序每孔加終止溶液50μl，終止反應（此時藍色立轉黃色）。終止液的加入順序應盡量與底物液的加入順序相同。為了保證實驗結果的準確性，底物反應時間到后應盡快加入終止液。
8.         用酶聯儀在450nm波長依序測量各孔的光密度（OD值）。 在加終止液后15分鐘以內進行檢測。
【大鼠白介素4(IL-4)ELISA試劑盒廠家】注：
1. 用戶在初次使用試劑盒時，應將各種試劑管離心數分鐘，以便試劑集中到管底。
2. 每次實驗留一孔作為空白調零孔，該孔不加任何試劑，只是*后加底物溶液及2N H2SO4。測量時先用此孔調OD值至零。
3. 為防止樣品蒸發，試驗時將反應板放于鋪有濕布的密閉盒內，酶標板加上蓋或覆膜。
4. 未使用完的酶標板或者試劑，請于2-8℃保存。標準品、生物素標記抗體工作液、辣根過氧化物酶標記親和素工作液請依據所需的量配置使用。請勿重復使用已稀釋過的標準品、生物素標記抗體工作液或、辣根過氧化物酶標記親和素工作液。
5. 建議檢測樣品時均設雙孔測定，以保證檢測結果的準確性。
以上資料僅供您參考，如有問題歡迎來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9240630 Power Supply, 5VDC 1.5 AMP, AP96        CNY            

9240593 Unload Valve, Pump, Pressure, AP196/98      CNY            

9240562 Fuse, PCB, 315, Eppendorf, AP196/98     CNY            

9240536 Motor Assy, SiNug, 17, All, AP96/196        CNY            

9240514 CD R/W, AP196/98        CNY            

9240504 PC-104, AP98        CNY            

9240346 Autopure, Full Agreement    "Repair service delivery: onsite

Service response time (repair): 48 hours/2 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"   CNY            

9240098 Re-Installation, BR EZ1 The Re-Installation, BR EZ1 facilitates quick implementation of system back into lab routine by minimizing  instrument set-up phase. It covers hardware ａnd software check of instrument ａnd provides user with operator refesher overview.  Re-installation includes labor ａnd travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.    CNY            

9239852 Foot bumpers,  v2.0 (4)     CNY            

9019944 Cable, P-axis, wires, 8 ch. , C18       CNY            

9238880 Bearings up. & low, BS96        CNY            

9238836 BioSprint96, Basic Agreement, no PM "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: not applicable"   CNY            

9241211 Full Agreement Depot Repair, DML2000, 1y    "The Full Agreement Depot Repair, DML2000 provides full service coverage for the DML 2000 luminometer. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."   CNY            

9241191 Magnet, tool, QX        CNY             【大鼠白介素4(IL-4)ELISA試劑盒廠家】

9238825 Spare bucket for plate rotor        CNY            

9238815 Rect. carrier for 12 tubes 10 ml w/ cap     CNY            

9019472 TAPE SPACER CLEAR GS        CNY            

9019464 UV tube, Cxx        CNY            

9243650 Comprehen Valid., EZ1/QIAcube/QIAsymph  """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   The Customer is expected to have all materials on-hand 1 week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """    CNY            

9237333 Install, BR plus 3 integr. w. scheduling    Installation ａnd training, BioRobot System including three approved integration devices with scheduling  CNY            

9237328 Upgrade, BR & 2 ext. devices w. sched.  Upgrade BioRobot with two approved integration devices using scheduling software CNY            

9237173 Shield, V-housing       CNY            

9233208 Tubing, liquid system 2.5/1.5       CNY            

9237155 V-Housing with Sample Needle        CNY            

9237150 Heater Top      CNY            

9016151 #App. Package, M48, Pathology       CNY            

9019179 Opto - plate lock closed, PyroQ96MD     CNY            

9011883 Upgrade, high speed pipetting system        CNY            

9011882 Back unit, BR9600       CNY            

9011823 Z-Modul; Tip-Adapter 300µl; BR8000      CNY             【大鼠白介素4(IL-4)ELISA試劑盒廠家】

9236916 3 Year Warranty, LiquiChip  LiquiChip 3-year Warranty with 1 Preventive Maintenance per year.  Included in system price.  This warranty must be included as a line item on the system quote.  It covers the system for three years total including all per incident travel ａnd labor charges, all repair parts, one preventive maintenance visits per year (three in total), ａnd 48- hour priority response.   CNY            

QY-x1169    人乳腺癌高轉移細胞MDA-MB-435                       

QY-x1170    人乳腺癌細胞MDA-MB-435S                    

QY-x1171    人乳腺癌細胞MDA-MB-436                     

QY-x1172    人乳腺導管癌細胞MDA-MB-453                     

QY-x1173    人乳腺細胞HBL-100                      

QY-x1174    人乳腺癌細胞BCaP-37                    

QY-x1175    人乳腺癌細胞LCC1                       

QY-x1176    人乳腺癌細胞MX-1                       

QY-x1177    人乳腺管癌細胞T47D                     

QY-x1178    人乳腺癌細胞SK-BR-3                     【大鼠白介素4(IL-4)ELISA試劑盒廠家】

QY-x1179    人乳腺癌細胞4T1                    

QY-x1180    人乳腺癌細胞HCC1937                    

QY-x1181    人乳腺導管癌細胞HCC38                      

QY-x1182    人乳腺癌細胞Hs-578T                    

QY-x1183    人乳腺癌Tamoxifen耐藥株LCC2                       

QY-x1184    人乳腺癌抗雌激素耐藥株LCC9                     

QY-x1185    人乳腺癌阿霉素耐藥細胞株MCF/Adr                    

QY-x1186    人乳腺癌細胞BT-549                     

QY-x1187    人乳腺導管瘤細胞BT-474                     

QY-x1188    人乳腺癌細胞MB-271                     

QY-x1189    人乳腺癌細胞ER-30                      

QY-x1190    人乳腺癌細胞M453                       

QY-x1191    人乳腺浸潤性導管癌旁皮膚細胞CCD-1095Sk                     

QY-x1192    人乳腺細胞HBL-100                      

QY-x1193    人乳腺導管瘤細胞UACC812                    

QY-x1194    人乳腺癌細胞系1590                      【大鼠白介素4(IL-4)ELISA試劑盒廠家】

QY-x1195    人卵巢癌細胞A2780                      

QY-x1196    人卵巢癌細胞Sw626                      

QY-x1197    人卵巢癌順鉑耐藥株COC1/CDDP                    

QY-x1198    人卵巢癌紫杉醇耐藥株A2780/Taxol                    

QY-x1199    人卵巢癌細胞系Anglne                       

QY-x1200    人卵巢癌細胞OVCaR-3                    

QY-x1201    人卵巢癌細胞Caov-3