齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1。我必須運行所有的標準和樣品的重復？

是的，重復的運行，以監測測定的精度和提高檢測結果的信心。

2。我必須在一次運行所有的樣品嗎？

不，每個試劑盒采用stripwell微孔板。這允許用戶在不同的時間來分析不同數量的樣品。

3。什么類型的可重復性的結果，得到與分析？

每一個工具都有一個包含一個典型數據的圖表的手冊。在運營商的任何變化，移液和洗滌技術，培養時間、溫度、試劑盒的年齡可以引起變化的結果。每個用戶都應該得到自己的標準曲線。

4。是否有可能儲存的試劑比表示？

在不推薦使用條件下的部件組件的存儲，以保證測試的正確性能。

5。我應該如何儲存我的樣品？

樣品應保存在-20°C或更低的溫度。長期儲存，建議凍結他們在- 70余。

6。我可以修改協議嗎？

BG的ELISA試劑盒進行了優化，提供*佳的結果。修改的格式或協議可能會給出錯誤的結果。

7。我可以使用一個不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗證在試劑盒插入的樣品類型。未經檢驗的樣品類型。為進一步信息的聯系技術服務。

8。我的樣本產生的值，在動態范圍內的測定。我可以使用這些值嗎？

建議只有在標準曲線的范圍內下降的樣本值。標準曲線的范圍內的值一般是非線性的，這可能導致不正確的外推值。產生值高于標準的樣品應為（進一步）稀釋和重復。如果樣品低于該測定的范圍內，該樣品被認為是不可檢測的。

9。我每次都要運行一個空白或零標準嗎？

是的，這些都是必需的計算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測到檢測。當排除特定的檢測問題的來源時，它們也非常有幫助。

10。我能改變我在檢測中使用的樣本量嗎？

不建議你改變卷由于BG工具被設計為*佳的性能在給定的體積

11�？梢允褂貌煌慕M件組件嗎？

每一個組件包含有特定批號的組件，以確保所有的組件都進行優化，以及與組件中的所有其他組件。對這些特定的地段進行質量檢驗。這是從來沒有建議使用你自己的組件或組件從其他包或供應商。

12。我的標準曲線看起來很好，但我沒有得到一個信號，我的樣本，當我預期，為什么？

樣品不包含被分析物。一個矩陣的效果可能是掩蓋檢測。確保推薦的稀釋液在試劑盒插入后按規定進行。如果建議稀釋，請檢查是否正確進行稀釋。過稀釋會導致樣品在標準曲線的范圍內下降。

13。你怎么推薦我洗盤子？

如果您使用的是一個自動平板洗衣機，我們建議定期檢查校準，并將該系統在洗滌前沖洗緩沖液沖洗。手動洗衣機也是一樣的。一個中繼器或洗瓶也可以使用。用戶應小心，以確保所有的內容都是送氣和板拍干的無塵紙。【大鼠抗精子抗體(AsAb)ELISA試劑盒現貨】

14。我需要用一個盤子嗎？

可靠的結果可以得到無板振動，但外徑的一般會低于那些使用平板振動器。

15。為什么我要用波長校正450-570nm之間？

對于酶聯免疫吸附試驗，在雙波長讀的是做正確的光密度貢獻的塑料以及，燈和光學波動。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預期的分析物濃度，我應該找到？

一個給定的分析物的量可能會有所不同，不僅從物種的物種，但也組織和細胞來源。這個信息的*佳來源是目前的文獻，很容易通過互聯網在多個科學數據庫訪問。

18。我的光密度有點高（或較低），比那些在手冊中，與我的試劑盒來了。為什么？

光學密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【大鼠抗精子抗體(AsAb)ELISA試劑盒現貨】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【大鼠抗精子抗體(AsAb)ELISA試劑盒現貨】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【大鼠抗精子抗體(AsAb)ELISA試劑盒現貨】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

9242763  QS RGQ, Full Agreement "Repair service delivery QIAsymphony SP/AS: onsite

Service response time (repair) QIAsymphony: 48 hours/2 business days

Repair service delivery RGQ: send to Regional Repair Center

Service response time (repair) RGQ: 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision RGQ: yes, included (provision time 2-3 days)

Transportation cost coverage: yes, full coverage"  CNY                             

9242547  SDS Canbus Cable, LC                 CNY                             

5000-1289        DML 2000 - Power Cord - 220V Switzerland             CNY                             

5000-1286        Fuse 2 AMP PKD 10, DML2                CNY                             

5000-1264        Cable Modem INST 9 PIN, DML2              CNY                             

5000-1507        Microplate Heater I - Fuse Inlet 3A 250V                  CNY                             

6000-00180      Plate Washer - Rinse Cap Assembly          CNY                             

6000-00179      Plate Washer - Wash Cap Assembly                   CNY                             

5000-1424        Brush Motor 2 Required, VTX2                  CNY                             

5000-1421        Block Support Back 099, VTX2                   CNY                             

5080-1000        Screw Caps                CNY                              【大鼠抗精子抗體(AsAb)ELISA試劑盒現貨】

PUREGST2        GST-tag Purification ａnd Detection Kit 2                   CNY                             

5000-1411        Poteneiometer 100L OHM, VTX2             CNY                             

6000-5026        hc2 Sample Conversion Tubes 15mL                  CNY                             

L2127       hc2 High-Risk HPV DNA Test PI (CD-ROM)                CNY                             

12943       QIAGEN Plasmid Plus Midi Kit (25)  "25 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 25 QIAfilter Midi Cartridges

QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction."    CNY                             

9240710  Filter, Pump, Jun-Air, Inlet/Outlet, AP               CNY                             

9240709  Filter, Pressure, 5 Micron, AP196/98                  CNY                             

9242094  QIAcube, Inspection Agreement     "Repair service delivery: not applicable

Service response time (repair): not applicable

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, for Periodic Inspection parts

Cost coverage for labor: yes, included for Periodic Maintenance

Cost coverage for travel: yes, included for Periodic Maintenance

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9242095  QIAxcel, Inspection Agreement                CNY                             

9241331  #QIAxcel, Full Agreement, no PM   The Full Agreement QIAxcel covers all travel, labor ａnd repair part costs related to an instrument repair for 1 year. Service response time is 2 working days.  CNY                             

9001772  careHPV test System                 CNY                             

275703     flocktype Salmonella Ab (5)               CNY                             

270045     cattletype BHV1 gB Ab (20)              CNY                             

274013     flocktype AIV Ab (5)          CNY                             

289993     virotype Tissue Lysis Reagent (250 ml)              CNY                             

281807     virotype CSFV RT-PCR Kit (480)                  CNY                              【大鼠抗精子抗體(AsAb)ELISA試劑盒現貨】

273403     pigtype Toxoplasma Ab (5)                CNY                             

9011875  ZLV Vacuum Trap               CNY                             

9016874  Kit, Maint. Prot. Plasticware, BR UNIV            CNY                             

9232338  Cable, RS232, internal chain, BR8000               CNY                             

9238945  H-motor, BS15          CNY                             

9238927  Linear conductor, BS15             CNY                             

DMEM     DMEM     DMEM with High-Glucose - L-Glutamine ａnd Sodium Pyruvate      500 ml                                 

DMEM  DMEM  DMEM with High-Glucose - L-Glutamine ａnd Sodium Pyruvate     1L                                

DMEM    DMEM    DMEM with L-Glutamine - Sodium Pyruvate ａnd 25 mM HEPES     500 ml                                 

DMEM  DMEM  DMEM with High-Glucose - L-Glutamine - Sodium Pyruvate ａnd 25 mM HEPES     500 ml                                 

DMEM-prf        DMEM-prf        DMEM with High Glucose - L-Glutamine ａnd Sodium Pyruvate - phenol red-free  1L                                

Ham＇s F-10       Ham＇s F-10       F-10 with L-Glutamine ａnd 25 mM HEPES      500 ml                                 

Ham＇s F-12       Ham＇s F-12       F-12 with L-Glutamine ａnd 25 mM HEPES      500 ml                                 

DMEM/F-12     DMEM/F-12     DMEM/F-12 with L-Glutamine        500 ml                                  【大鼠抗精子抗體(AsAb)ELISA試劑盒現貨】

DMEM/F-12    DMEM/F-12    DMEM/F-12 with L-Glutamine ａnd 15 mM HEPES          500 ml                                 

RPMI 1640       RPMI 1640       RPMI  1640 without L-Glutamine - with 25 mM HEPES        500 ml                                 

RPMI 1640       RPMI 1640       RPMI  1640 with L-Glutamine ａnd 25 mM HEPES          500 ml                                 

IMDM    IMDM    MDM with L-Glutamine ａnd 25 mM HEPES; without alpha-Thioglycerol - 2-mercaptoethanol   500 ml                                 

rhSCF        重組人干細胞因子         Recombinant Human Stem Cell Factor    2µg                             

rhG-CSF    重組人粒細胞集落刺激因子         Recombinant Human Granulocyte Colony Stimulating Factor         2µg                             

rhGM-CSF         重組人粒細胞巨噬細胞集落刺激因子         Recombinant Human Granulocyte Macrophage Colony Stimulating Factor         5µg                                     

rhM-CSF   重組人巨噬細胞集落刺激因子     Recombinant Human Macrophage Colony Stimulating Factor        2µg                             

rhTNF-α 重組人腫瘤壞死因子-α        Recombinant Human Tumor Necrosis Factor-alpha         10µg                                    

rhTNF-α-His    重組人腫瘤壞死因子-α,His Recombinant Human Tumor Necrosis Factor-alpha,His  10µg                                    

rhTNF-α Vari 重組人腫瘤壞死因子-αVariant Recombinant Human Tumor Necrosis Factor- alpha Variant          10µg                                    

rhBAF       重組人B細胞活化因子         Recombinant Human B Cell Activating Factor         5µg                                     

rhOSM      重組人抑瘤素-M    Recombinant Human Oncostatin-M        2µg                             

rhOSM 209a.a.         重組人抑瘤素-M209a.a.        Recombinant Human Oncostatin-M 209a.a.  2µg                             

rhAK1-3    重組人血管抑素K1-3     Recombinant Human Angiostatin K1-3   10µg                                    

rhEndostatin    重組人血管內皮抑素     Recombinant Human Endostatin     2µg                              【大鼠抗精子抗體(AsAb)ELISA試劑盒現貨】

rhaFGF     重組人成纖維細胞生長因子－－酸性       Recombinant Human Fibroblast Growth Factor- acidic  10µg                                    

rhbFGF     重組人成纖維細胞生長因子－－堿性       Recombinant Human Fibroblast Growth Factor-basic    10µg                                    

rhKGF-1    重組人角質細胞生長因子-1 Recombinant Human Keratinocye Growth Factor-1        2µg                             

rhFGF-9    重組人成纖維細胞生長因子-9      Recombinant Human Fibrobalst Growth Factor-9  5µg                                     

rhKGF-2    重組人角質細胞生長因子-2 "Recombinant Human Keratinocye Growth Factor-2

"       5µg                             

rhFGF-19  重組人成纖維細胞生長因子-19   "Recombinant Human Fibrobalst Growth Factor-19

"       5µg                                     

rhFGF-21  重組人成纖維細胞生長因子-21   "Recombinant Human Fibrobalst Growth Factor-21

"       5µg