價格詳細資料：試劑盒價格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認同。齊一生物大品牌保證，傾力為國內外科研院校實驗室提供*優質產品。

【小鼠小腸結腸炎耶爾森菌抗體(YersiniaenterocoliticaAb）ELISA試劑盒廠家】注意事項

1. 當混合蛋白溶液時應盡量輕緩，避免起泡。

2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。

3. 一次加樣時間控制在5分鐘內，如標本數量多，推薦使用排槍加樣。

4. 請每次測定的同時做標準曲線，做復孔。

5. 如標本中待測物質含量過高，請先稀釋后再測定，計算時請*后乘以稀釋倍數。

6. 在配制標準品、檢測溶液工作液時，請以相應的稀釋液配制，不能混淆。

7. 底物請避光保存。

8. 不要用其它生產廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【小鼠小腸結腸炎耶爾森菌抗體(YersiniaenterocoliticaAb）ELISA試劑盒廠家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【小鼠小腸結腸炎耶爾森菌抗體(YersiniaenterocoliticaAb）ELISA試劑盒廠家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【小鼠小腸結腸炎耶爾森菌抗體(YersiniaenterocoliticaAb）ELISA試劑盒廠家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

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9236359  Sheath Filter with Quick Disconnect                  CNY                             

9236346  1.5A Fuse LiquiChip           CNY                             

9236338  Quick disconnect Set                  CNY                             

9236164  Syringe block, repl. kit, RapidPlate           CNY                             

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9235296  Capillary, Dispenser head, Mounting Tool                 CNY                             

9235054  Incubator Tower (65°C) Board                   CNY                             

9234941  Incubator Tower (65°C) Board DINCS                CNY                             

9238658  Cooling compressor T2178GK, 50Hz                  CNY                             

9242060  Adapter plate, IQ/OQ, QS                 CNY                             

9242026  Repair Thermo, Depot Repair, Palm Cycler              CNY                             

9241824  Pyro Q96 MD, Full Agreement         The Full Agreement, PyM provides full service coverage for the PyroMark instrument. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one on-site inspection service, with costs for labor ａnd parts covered. With a Full Agreement, there is a response time of 2 working days.         CNY                             

9241815  Pyro Q96 ID, PM Agreement            CNY                              【小鼠小腸結腸炎耶爾森菌抗體(YersiniaenterocoliticaAb）ELISA試劑盒廠家】

9241172  anti-rotation tool              CNY                             

9240960  Cable, cLLD, pipetting system, Qsym                CNY                             

9240949  Cable, y, portal, Qsym               CNY                             

9240678  Bimba Rotary Actuator, AP               CNY                             

9240777  CE Update, AP196/98                 CNY                             

9241773  RGQ, Installation     The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the Rotorgene-Q systems hardware ａnd trains the user on the operation of the Rotorgene software. After the training the user will be able to develop application programs, conduct a basic troubleshooting, ａnd understand the application output format.All travel ａnd labor costs ａnd well as material for the demonstration run are included. The service is documented in accordance with GMP/GLP requirements.     CNY                             

9241763  QIAgility, Full Agreement         "Repair service delivery: onsite

Service response time (repair): 48 hours/2 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) or PC repair provision: not included

Transportation cost coverage: not applicable"        CNY                             

9241372  Reflector, UV lamp, EZ1a                  CNY                             

9243545  QIAsymphony SP, PM Service   "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9243045  Bracket, rear, transparent panel, Qsym           CNY                             

9242980  QIArent&go - Rent out fee QS RGQ System              CNY                             

9242764  QS RGQ, Premium Agreement         "Repair service delivery QIAsymphony SP/AS: onsite

Service response time (repair) QIAsymphony: 24 hours/1 business day

Repair service delivery RGQ: send to Regional Repair Center

Service response time (repair) RGQ: 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision RGQ: yes, included (provision time 2-3 days)

Transportation cost coverage: yes, full coverage

QIAsymphony Response Time starts when customer inquiry is received by local tech service ends when a service specialist is onsite.

- Inquiry Channel: Local Tech-Service Hotline, Mo.- Fr. 08:00-17:00 Uhr

- Call Cut-off Time: 12:00 am; on business days

- Customer Availability: maximum 2 hours phone trouble shooting to identify error ａnd spare parts

- QIAsense: Systems need to be connected to QIAsense for faster download of log files

- Software: QIAsymphony need to be updated with the latest software version

- Remote Area: 24 Hr onsite response only available for geographical areas within field service travel capability ａnd pre-12:00 delivery capability of spare parts"         CNY                             

9242750  Cooling adapter, PCR adapter, AS, Qsym                   CNY                             

9242709  Validation Support, 2d, EZ1     The QIAGEN EZ1 Guided Validation Support service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the EZ1 system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, ａnd practical hands-on operation.  The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates. The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support.   CNY                             

9242699  QIAxcel, Full Agreement "Repair service delivery send to Regional Repair Center

Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery:

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: yes, included (provision time 2-3 days)

Transportation cost coverage: yes, full coverage"  CNY                             

QY-x1169 人乳腺癌高轉移細胞MDA-MB-435                                                   

QY-x1170 人乳腺癌細胞MDA-MB-435S                                                      

QY-x1171 人乳腺癌細胞MDA-MB-436                                               

QY-x1172 人乳腺導管癌細胞MDA-MB-453                                               

QY-x1173 人乳腺細胞HBL-100                                                    

QY-x1174 人乳腺癌細胞BCaP-37                                                

QY-x1175 人乳腺癌細胞LCC1                                                      

QY-x1176 人乳腺癌細胞MX-1                                                      

QY-x1177 人乳腺管癌細胞T47D                                                 

QY-x1178 人乳腺癌細胞SK-BR-3                                                  【小鼠小腸結腸炎耶爾森菌抗體(YersiniaenterocoliticaAb）ELISA試劑盒廠家】

QY-x1179 人乳腺癌細胞4T1                                               

QY-x1180 人乳腺癌細胞HCC1937                                              

QY-x1181 人乳腺導管癌細胞HCC38                                                   

QY-x1182 人乳腺癌細胞Hs-578T                                                

QY-x1183 人乳腺癌Tamoxifen耐藥株LCC2                                               

QY-x1184 人乳腺癌抗雌激素耐藥株LCC9                                                  

QY-x1185 人乳腺癌阿霉素耐藥細胞株MCF/Adr                                               

QY-x1186 人乳腺癌細胞BT-549                                                  

QY-x1187 人乳腺導管瘤細胞BT-474                                                  

QY-x1188 人乳腺癌細胞MB-271                                                

QY-x1189 人乳腺癌細胞ER-30                                                    

QY-x1190 人乳腺癌細胞M453                                                    

QY-x1191 人乳腺浸潤性導管癌旁皮膚細胞CCD-1095Sk                                                  

QY-x1192 人乳腺細胞HBL-100                                                    

QY-x1193 人乳腺導管瘤細胞UACC812                                                       

QY-x1194 人乳腺癌細胞系1590                                                  【小鼠小腸結腸炎耶爾森菌抗體(YersiniaenterocoliticaAb）ELISA試劑盒廠家】

QY-x1195 人卵巢癌細胞A2780