齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1。我必須運行所有的標準和樣品的重復？

是的，重復的運行，以監測測定的精度和提高檢測結果的信心。

2。我必須在一次運行所有的樣品嗎？

不，每個試劑盒采用stripwell微孔板。這允許用戶在不同的時間來分析不同數量的樣品。

3。什么類型的可重復性的結果，得到與分析？

每一個工具都有一個包含一個典型數據的圖表的手冊。在運營商的任何變化，移液和洗滌技術，培養時間、溫度、試劑盒的年齡可以引起變化的結果。每個用戶都應該得到自己的標準曲線。

4。是否有可能儲存的試劑比表示？

在不推薦使用條件下的部件組件的存儲，以保證測試的正確性能。

5。我應該如何儲存我的樣品？

樣品應保存在-20°C或更低的溫度。長期儲存，建議凍結他們在- 70余。

6。我可以修改協議嗎？

BG的ELISA試劑盒進行了優化，提供*佳的結果。修改的格式或協議可能會給出錯誤的結果。

7。我可以使用一個不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗證在試劑盒插入的樣品類型。未經檢驗的樣品類型。為進一步信息的聯系技術服務。

8。我的樣本產生的值，在動態范圍內的測定。我可以使用這些值嗎？

建議只有在標準曲線的范圍內下降的樣本值。標準曲線的范圍內的值一般是非線性的，這可能導致不正確的外推值。產生值高于標準的樣品應為（進一步）稀釋和重復。如果樣品低于該測定的范圍內，該樣品被認為是不可檢測的。

9。我每次都要運行一個空白或零標準嗎？

是的，這些都是必需的計算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測到檢測。當排除特定的檢測問題的來源時，它們也非常有幫助。

10。我能改變我在檢測中使用的樣本量嗎？

不建議你改變卷由于BG工具被設計為*佳的性能在給定的體積

11�？梢允褂貌煌慕M件組件嗎？

每一個組件包含有特定批號的組件，以確保所有的組件都進行優化，以及與組件中的所有其他組件。對這些特定的地段進行質量檢驗。這是從來沒有建議使用你自己的組件或組件從其他包或供應商。

12。我的標準曲線看起來很好，但我沒有得到一個信號，我的樣本，當我預期，為什么？

樣品不包含被分析物。一個矩陣的效果可能是掩蓋檢測。確保推薦的稀釋液在試劑盒插入后按規定進行。如果建議稀釋，請檢查是否正確進行稀釋。過稀釋會導致樣品在標準曲線的范圍內下降。

13。你怎么推薦我洗盤子？

如果您使用的是一個自動平板洗衣機，我們建議定期檢查校準，并將該系統在洗滌前沖洗緩沖液沖洗。手動洗衣機也是一樣的。一個中繼器或洗瓶也可以使用。用戶應小心，以確保所有的內容都是送氣和板拍干的無塵紙。【小鼠γ干擾素(IFN-γ)ELISA試劑盒價格】

14。我需要用一個盤子嗎？

可靠的結果可以得到無板振動，但外徑的一般會低于那些使用平板振動器。

15。為什么我要用波長校正450-570nm之間？

對于酶聯免疫吸附試驗，在雙波長讀的是做正確的光密度貢獻的塑料以及，燈和光學波動。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預期的分析物濃度，我應該找到？

一個給定的分析物的量可能會有所不同，不僅從物種的物種，但也組織和細胞來源。這個信息的*佳來源是目前的文獻，很容易通過互聯網在多個科學數據庫訪問。

18。我的光密度有點高（或較低），比那些在手冊中，與我的試劑盒來了。為什么？

光學密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【小鼠γ干擾素(IFN-γ)ELISA試劑盒價格】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【小鼠γ干擾素(IFN-γ)ELISA試劑盒價格】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【小鼠γ干擾素(IFN-γ)ELISA試劑盒價格】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

9238815  Rect. carrier for 12 tubes 10 ml w/ cap            CNY                             

9019472  TAPE SPACER CLEAR GS             CNY                             

9019464  UV tube, Cxx             CNY                             

9243650  Comprehen Valid., EZ1/QIAcube/QIAsymph    """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   The Customer is expected to have all materials on-hand 1 week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """     CNY                             

9237333  Install, BR plus 3 integr. w. scheduling     Installation ａnd training, BioRobot System including three approved integration devices with scheduling   CNY                             

9237328  Upgrade, BR & 2 ext. devices w. sched.  Upgrade BioRobot with two approved integration devices using scheduling software  CNY                             

9237173  Shield, V-housing               CNY                             

9233208  Tubing, liquid system 2.5/1.5             CNY                             

9237155  V-Housing with Sample Needle                  CNY                             

9237150  Heater Top                CNY                             

9016151  #App. Package, M48, Pathology               CNY                             

9019179  Opto - plate lock closed, PyroQ96MD               CNY                             

9011883  Upgrade, high speed pipetting system             CNY                             

9011882  Back unit, BR9600            CNY                             

9011823  Z-Modul; Tip-Adapter 300µl; BR8000               CNY                              【小鼠γ干擾素(IFN-γ)ELISA試劑盒價格】

9236916  3 Year Warranty, LiquiChip       LiquiChip 3-year Warranty with 1 Preventive Maintenance per year.  Included in system price.  This warranty must be included as a line item on the system quote.  It covers the system for three years total including all per incident travel ａnd labor charges, all repair parts, one preventive maintenance visits per year (three in total), ａnd 48- hour priority response.     CNY                             

9019143  Power supply, PyroQ96             CNY                             

9019129  Cable - Reagent, PyroQ96ID             CNY                             

9019126  PCB 2 - Connection, PyroQ96ID                 CNY                             

9019114  IPC mainboard, PyroQ96ID               CNY                             

9019025  Worktable, side wall, left, EZ1xl                CNY                             

9019012  Motor Y, EZ1xl          CNY                             

9018942  Adapter, 2xRing of 12 COBAS Amplicore          CNY                             

9022737  Rotor-Gene AssayManager SOW              CNY                             

9016061  Tubing Set, Syringe inlet, BR8000 I           CNY                             

9015823  CD, Field Syringe Calibration, BR8000              CNY                             

9015690  Rings (10), pinching, 4.5/3.2 mm               CNY                             

9015599  CD, Manual, Service, BIOROBOT 8000 II                   CNY                             

9015587  EZ1 DNA Buffy Coat Card         Pre-programmed card for BioRobot EZ1 DNA Buffy Coat Protocol          CNY                             

9019166  Lead screw - X, PyroQ96MD             CNY                             

QY-x950   人結腸腺癌細胞HCT-8                                                

QY-x951   耐長春新堿結腸癌細胞系HCT-8/VCR                                                 【小鼠γ干擾素(IFN-γ)ELISA試劑盒價格】

QY-x952   人結腸腺癌細胞HT-29                                                

QY-x953   人十二指腸腺癌細胞HuTu-80                                                    

QY-x954   人結腸癌細胞Loo                                               

QY-x955   人結腸癌細胞SW480                                                  

QY-x956   人結腸癌細胞SW620                                                  

QY-x957   人結腸癌細胞T84                                               

QY-x958   人結腸癌細胞Lovo                                                       

QY-x959   結直腸癌細胞LOVE1                                                   

QY-x960   人結腸癌細胞Ls-174-T                                                

QY-x961   人結腸癌細胞LS174T                                                  

QY-x962   人結腸癌細胞CW-2                                                      

QY-x963   人結腸癌細胞RKO                                              

QY-x964   人結腸癌轉基因細胞RKO-AS45-1                                                       

QY-x965   人結腸癌轉基因細胞RKO-E6                                                      

QY-x966   人大腸癌細胞CL187/CCL187                                               

QY-x967   人大腸癌細胞SW116                                                   【小鼠γ干擾素(IFN-γ)ELISA試劑盒價格】

QY-x968   人大腸癌細胞PA319                                                    

QY-x969   人盲腸腺癌細胞（未分化）Hce-8693                                              

QY-x970   人結直腸腺癌細胞HCT-15                                                  

QY-x971   人結直腸腺癌細胞LS174T                                                  

QY-x972   人結腸腺癌細胞LS180                                                

QY-x973   人高分化結腸腺癌細胞系THC-8307                                                  

QY-x974   人結直腸腺癌上皮細胞DLD-1                                                    

QY-x975   人小腸癌細胞系HIC                                                    

QY-x976   人結腸癌細胞HRT-S1