1.購買我公司任意ELISA試劑盒指標享受免費代測，并提供技術指導服務！

二、實驗材料與試劑配制：

1. 儀器與材料：酶標儀（使用前預熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時備用（如果標本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【小鼠血紅素氧合酶1(HO-1)ELISA試劑盒廠家】三. 仔細閱讀說明書

1.檢查試劑盒標簽上的有效期。如果超過有效期，請勿使用。

2. 按說明書確定所有試劑齊全（數量、體積）

3. 標本制備要規范，每份標本體積要按2-3個復孔以上的量制備（貯存），盡量分裝做備份。

4.短期內無法實驗者，注意低溫保存

5.準備好所有實驗額外所需物品，（比如移液器，試管，清洗器，酶標儀）

6. 按說明書將所用試劑平衡至室溫，根據檢測標本數量確定所需試劑的量

7.檢查試劑盒內每種試劑的貯存條件，保證所有試劑均按照試劑盒內說明書推薦的條件存儲。 【小鼠血紅素氧合酶1(HO-1)ELISA試劑盒廠家】

8.檢查不穩定或者變質的試劑溶液（比如沉淀或變色）。有些試劑，比如標準品稀釋液或者檢測稀釋液，可能在設計時就含有沉淀物。所有試劑在滴入酶標板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標板之前，必需不停攪拌。

9.保證充分的孵育時間和溫度。

10.切勿使用不同生產批號的試劑取代現有試劑或混合現有試劑。

11.在混合或溶解蛋白溶液時，避免泡沫產生。

12.在實驗開始之前，安排好實驗流程。在實驗開始之前，清潔工作臺。

13.若有問題，應與我公司或代理商的技術支持聯系

【小鼠血紅素氧合酶1(HO-1)ELISA試劑盒廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩定。我們提倡新鮮標本盡早檢測，對收集后當天就進行檢測的標本，及時儲存在4℃備用，如有特殊原因需要周期收集標本，請造模取材后，將標本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質量，所以避免反復凍融。部分標本也可加入適當防腐劑，激素類標本需添加抑肽酶。 

2.試劑盒代測標本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項請與我司技術人員溝通。

3.液體類標本：標本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細胞培養上清、組織勻漿等。

4.購買前請提前告訴我們：您樣本的種屬、樣本數量、待測指標及其它特殊要求。

【小鼠血紅素氧合酶1(HO-1)ELISA試劑盒廠家】五、代測數據詳細內容

1、ELISA標準曲線；

2、實驗具體詳細步驟；

3、完整的實驗報告（試劑耗材，實驗方法，實驗結果及結果說明）；

4、實驗進行階段，我公司客服人員會及時向您匯報實驗進程。

9018133  Sensor, rodcover, extractor Qsym             CNY                              【小鼠血紅素氧合酶1(HO-1)ELISA試劑盒廠家】

9018125  Magnet plate, extractor, Qsym                 CNY                             

9018122  Set, fuses F1, F2, F3, mainboard, QIAC             CNY                             

9017834  Module, shaker, complete, QIAC              CNY                             

9017816  Motor, rotary, gripper, QIAC             CNY                             

9017791  Motor, Z-drive, gripper, complete, QIAC                   CNY                             

9017763  User Manual EZ1 DSP                CNY                             

9015210  Z-Module 1100µl, BR8 RD                 CNY                             

9015180  Cooling Block 48-tube, 1.4 ml, M48                   CNY                             

9015177  Tip Rack, M48          CNY                             

9015018  Probe 0.9mm, BR8 RD              CNY                             

9018095  QIAcube Transport Box             CNY                             

9017835  Cable, flat, shaker, 16 pol. , QIAC              CNY                             

9017817  Actuator, gripper, QIAC            CNY                             

9017806  Body, roof, QIAC                CNY                             

9017785  Board, Y, QIAC          CNY                             

9015183  Tip Loader, M96                CNY                              【小鼠血紅素氧合酶1(HO-1)ELISA試劑盒廠家】

9015289  Motor, X/Y                 CNY                             

1030481  Caps forElution Microtubes(55x8),PAX,FLU               CNY                             

133003     NeXtalStock Methylpentanediol (200)              CNY                             

9243570  Centrifuge, PM Agreement     "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9243730  DML Installation ａnd Software Training  "4hr. Installation ａnd Suite Software training includes: Training Installation, Operation, ａnd Maintenance of DML 3000

DML 3000 Features, ａnd Basic Troubleshooting

Suite Software Installation ａnd Operation

Non routine Functions

Routine Functions

Assay Protocols

Creating ａnd Measuring Plates

Analyzing results

All labor, travel cost ａnd training documentation included."  CNY                             

9014380  Reagent Holder, micortubes ａnd troughs        Holder for accommodating 8 microtubes (1.5 ml), 4 microtubes (2 ml), ａnd 1 disposable trough (20 ml). For use with Buffers AL, AVE, ａnd TOPE on the BioRobot MDx worktable   CNY                             

9014325  spez. Abdeckblech Transport MWG (9194)               CNY                             

9018284  Brackets Kit, RCS 4-Plate Shaker               CNY                             

9018212  MEL sensor flag, M48                CNY                             

9018167  PCB, connect ebox, chassis, Qsym            CNY                             

9018162  PCB, connect, touch screen, Qsym           CNY                             

9018123  Tool, cable, FIW ｕpdate centrifuge, QIAC                 CNY                             

9018089  Cooling adapter, Reagent holder 2, Qsym                 CNY                             

9017854  Shaker Rack Plugs (12)              CNY                             

9017847  Shaker Rack               CNY                             

QY-03790 IL-6(Interleukin-6)白介素6（白細胞介素-6）(多肽抗原)              0.5mg/6501mg/1100                                  

QY-06980 IL-10(Interleukin-10)白介素10（白細胞介素-10）(抗原)              0.5mg/6851mg/1100                                  

QY-0698   IL-10(Interleukin-10)白介素10（白細胞介素-10）(抗原)              0.5mg/6501mg/1100                                  

QY-07800 IL-8白介素8（白細胞介素-8）(抗原)           0.5mg/650                                  

QY-06810 Insulin Receptor（ISR）胰島素受體（抗原）                0.5mg/6501mg/1100                                  

QY-06410 ITG-α(Integrin α chain)整合素-α（抗原）               0.5ml/6501ml/1100                                    

QY-07050 LDL-R(Low-density lipoprotein receptor precursor)低密度脂蛋白受體(抗原)                 0.5mg/6501mg/1100                                 

QY-07220 Ki-67 AntigenKi-67 Antigen(抗原)             0.5mg/6501mg/1100                                  

QY-07490 Kiss-1(Metastasis-suppressor KiSS-1 precursor)腫瘤轉移抑制基因（抗原)           0.5mg/6501mg/1100                                  【小鼠血紅素氧合酶1(HO-1)ELISA試劑盒廠家】

QY-08640 重組K3蛋白             0.5mg/650                                  

QY-04090 Leptin瘦素（抗原)                    0.5mg/6501mg/1100                                  

QY-07590 Lpin1 proteinLpin1 protein(抗原)              0.5mg/6501mg/1100                                  

QY-04840 Lumbrokinase蚯蚓纖溶酶(蚓激酶抗原)                  0.5mg/6501mg/1100                                  

QY-07380 Livin （Inhibitors of apoptosis proterins Livin）一種新的凋亡抑制蛋白(抗原)             0.5mg/6501mg/1100                                 

QY-06370 p38 MAPK絲裂原活化蛋白激酶p38(抗原)            0.5mg/6501mg/1100                                  

QY-04010 Metal ion transporter擬南介金屬離子轉運蛋白(抗原)                 0.5mg/6501mg/1100                                  

QY-06420 MADCAM-1黏膜選址素（抗原）                    0.5mg/6501mg/1100                                  

QY-07920 Maspin (mammary serine protease inhibitor)抑癌基因(抗原)                  0.5ml/6501ml/1100                                    

QY-02570 MAG-a/b (Myelin associated glycoprotein; L / S -MAG;)髓鞘相關糖蛋白a/b(抗原)               0.5mg/6501mg/1100                                  

QY-02580 MAG (Myelin associated glycoprotein; L-MAG;MAG-a )髓鞘相關糖蛋白(抗原)             0.5mg/6501mg/1100                                 

QY-02580 MAG-a (Myelin associated glycoprotein; L-MAG; )髓鞘相關糖蛋白-a(抗原)                   0.5mg/650                                  

QY-04630 MMP-1(Mous ematrix metalloproteinases-1)基質金屬蛋白酶-1（小鼠抗原）            0.5mg/6501mg/1100                                 

QY-07610 midnolin isoform Protein 1中腦核仁蛋白1（抗原）             0.5mg/6501mg/1100                                  

QY-07620 midnolin isoform Protein 2中腦核仁蛋白2（抗原）             0.5mg/6501mg/1100                                  

QY-08320 MICA(MHC class I polypeptide-related sequence A)一種細胞應激分子抗原                  0.5mg/6501mg/1100                                  【小鼠血紅素氧合酶1(HO-1)ELISA試劑盒廠家】

QY-06560 MRP3(Multidrug Resistanec-Associated Protein 3)多藥耐藥相關蛋白3(抗原)              0.5mg/6501mg/1100                                 

QY-06570 MRP1(Multidrug Resistanec-Associated Protein 1)多藥耐藥相關蛋白1(抗原)              0.5mg/6501mg/1100                                 

QY-06610 LRP/MVP (Lung resistance related protein)肺耐藥相關蛋白(抗原)                 0.5mg/6501mg/1100                                  

QY-03000 MSLN(mesothelin)間皮素(多肽抗原)               0.5mg/6501mg/1100                                  

QY-07580 MSH2 (mutS homolog 2)MSH2抗原                  0.5mg/6501mg/1100