檢測標本：血清,血漿,尿液,胸腹水,腦脊液,細胞培養上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯免疫夾心法

產品的用途：僅供科研究課題使用

價格及詳細資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認同.大品牌保證,價格公道,傾力為國內外科研院校實驗室提供*優質的產品.若有需要,我司將竭誠為您服務！本試劑盒用于測定血清,血漿及相關液體樣本中含量.

【豬腦鈉素/腦鈉尿肽(BNP)ELISA 試劑盒現貨】實驗原理：

   本試劑盒應用雙抗體夾心法測定標本中該產品水平.用純化的本產品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產品抗原,再與HRP標記的本產品抗體結合,形成抗體-抗原-酶標抗體復合物,經過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉化成藍色,并在酸的作用下轉化成*終的黃色.顏色的深淺和樣品中的本產品呈正相關.用酶標儀在450nm波長下測定吸光度（OD值）,通過標準曲線計算樣品中該產品濃度.

試劑盒組成：

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【豬腦鈉素/腦鈉尿肽(BNP)ELISA 試劑盒現貨】標本要求：

1．標本采集后盡早進行提取,提取按相關文獻進行,提取后應盡快進行實驗.若不能馬上進行試驗,可將標本放于-20℃保存,但應避免反復凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標準品的稀釋與加樣：在酶標包被板上設標準品孔10孔,在、第二孔中分別加標準品100μl,然后在、第二孔中加標準品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標準品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標準品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標準品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標準品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）. 【豬腦鈉素/腦鈉尿肽(BNP)ELISA 試劑盒現貨】

2.     加樣：分別設空白孔（空白對照孔不加樣品及酶標試劑,其余各步操作相同）、待測樣品孔.在酶標包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標板孔底部,盡量不觸及孔壁,輕輕晃動混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復5次,拍干.

6.     加酶：每孔加入酶標試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應（此時藍色立轉黃色）.

11. 測定：以空白空調零,450nm波長依序測量各孔的吸光度（OD值）. 測定應在加終止液后15分鐘以內進行.

注意事項：

1． 試劑盒從冷藏環境中取出應在室溫平衡15-30分鐘后方可使用,酶標包被板開封后如未用完,板條應裝入密封袋中保存.

2． 濃洗滌液可能會有結晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結果. 【豬腦鈉素/腦鈉尿肽(BNP)ELISA 試劑盒現貨】

3． 各步加樣均應使用加樣器,并經常校對其準確性,以避免試驗誤差.一次加樣時間控制在5分鐘內,如標本數量多,推薦使用排搶加樣.

4． 請每次測定的同時做標準曲線,做復孔.如標本中待測物質含量過高（樣本OD值大于標準品孔孔的OD值）,請先用樣品稀釋液稀釋一定倍數（n倍）后再測定,計算時請*后乘以總稀釋倍數（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請避光保存.

7． 嚴格按照說明書的操作進行,試驗結果判定必須以酶標儀讀數為準.

8． 所有樣品,洗滌液和各種廢棄物都應按傳染物處理.

9． 本試劑不同批號組分不得混用.

10. 如與英文說明書有異,以英文說明書為準.

計算：

以標準物的濃度為橫坐標,OD值為縱坐標,   

在坐標紙上繪出標準曲線,根據樣品的OD     

值由標準曲線查出相應的濃度；再乘以稀釋     

倍數；或用標準物的濃度與OD值計算出標     

準曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計算出樣品濃度,再乘以稀釋

倍數,即為樣品的實際濃度.

試劑盒性能：

1.樣品線性回歸與預期濃度相關系數R值為0.95以上.

2.批內與批見應分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個月

291115     mericon RR Soy Kit (96)            CNY                              【豬腦鈉素/腦鈉尿肽(BNP)ELISA 試劑盒現貨】

291065     mericon GMO Screen bar (96)                   CNY                             

9001933  DML 3000 120-240V Q              CNY                             

9001915  PyroMark Q96 ID, Priority        PyroMark Q96 ID, Priority includes the PyroMark Q96 ID instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents. CNY                             

133120     NeXtalStock Tricine (200)                   CNY                             

133180     NeXtalStock Citric acid pH 3.0 (200)                  CNY                             

9018503  Manual air supply, service tool, QX           CNY                             

51161       QIAamp 96 DNA Blood Kit (4) For 4 x 96 DNA preps: 4 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels   CNY                             

133106     NeXtalStock Sodium ac. H2O pH 4.6 (200)                CNY                             

9015519  Spring, for door                  CNY                             

9001897  QIAxtractor, UV/HEPA (w/o PC) Priority  QIAxtractor, UV/HEPA (w/o PC) Priority includes the QIAxtractor instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service ａnd inspection is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.  CNY                             

9001875  EZ1 Advanced XL, Priority        EZ1 Advanced XL, Priority includes the EZ1 Advanced XL instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents. CNY                             

959236     MagAttract RNA Tissue Mini M48 Kit (192)             CNY                             

956336     MagAttract RNA Univ. Tissue M48 Kit(192)             CNY                             

9018457  Cartridge, alignment tool, QX          CNY                             

9015467  Motor, P            CNY                             

9015451  Modules, DISU, BR8000 v2.1            CNY                              【豬腦鈉素/腦鈉尿肽(BNP)ELISA 試劑盒現貨】

9012427  Thermostate system, complete, 120V, BR30           CNY                             

9018483  Notebook, QX           CNY                             

51297       FlexiGene DNA AGF3000 Kit (640)  "Buffer FG1  = 4 x 825 ml

Buffer FG2 = 1 x 750 ml

Buffer FG3 = 10 x 625 ml

QIAGEN Protease = 5 vials

1 handbook"    CNY                             

51185       QIAamp DNA Blood Midi Kit (100)  For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml)   CNY                             

38075       AIL Cartridge (5ml)           CNY                             

9015471  Sensor, photo, PM-U24             CNY                             

956034     EZ1 RNA Universal Tissue Kit (48)   Provides automated purification of total RNA from 1-6 samples of all types of animal or human tissue, including difficult-to-lyse fibrous ａnd fatty tissues, or cultures cells.         CNY                             

204772     Rotor-Gene Multiplex PCR Kit (80)  The Rotor-Gene Multiplex PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly reliable quantification in multiplex, real-time PCR ａnd two-step RT-PCR using sequence-specific probes. Up to 4 genes (e.g., 1 control gene ａnd 3 target genes) can be quantified simultaneously in the same reaction.       CNY                             

9018435  Tubing, external N2 source, 10ft, QX                CNY                             

9018417  Cover, front, blue window, QX          CNY                             

9018403  ZLV, Detect barrel (1), QX                  CNY                             

290103     mericon Shigella spp Kit (24)             CNY                             

9015392  Clamp, rubber, adapter plate, HS shaker,                 CNY                             

9001793  QIAcube HT/QIAxtractor, system              CNY                             

9012330  Syringe Pump outlet tubing               CNY                             

QKT0965  尿道炎熒光檢測試劑盒                                                       

QKT0966  淋病熒光檢測試劑盒                                                  

QKT0967  乙型肝炎病毒核心IgM抗體檢測試劑盒                                                  

EK0301     hActA-ELISA     人活化素A酶聯免疫試劑盒         Human Activin A ELISA Kit       96 tests                      

EK0305     hANG-ELISA     人雙調蛋白酶聯免疫試劑盒         Human ANG ELISA Kit      96 tests                      

EK0307     hBDNF-ELISA   人腦源性神經生長因子酶聯免疫試劑盒     Human BDNF ELISA Kit    96 tests                      

EK0310     hBMP-5-ELISA 人BMP-5酶聯免疫試劑盒    Human BMP-5 ELISA Kit  96 tests                      

EK0311     hBMP-2-ELISA 人BMP-2酶聯免疫試劑盒    Human BMP-2 ELISA Kit  96 tests                       【豬腦鈉素/腦鈉尿肽(BNP)ELISA 試劑盒現貨】

EK0314     hBMP-4-ELISA 人BMP-4酶聯免疫試劑盒    Human BMP-4 ELISA Kit  96 tests                      

EK0325     hEGF-ELISA       人表皮生長因子酶聯免疫試劑盒         Human EGF ELISA Kit       96 tests                      

EK0327     hEGFR-ELISA    人表皮生長因子受體酶聯免疫試劑盒         Human EGFR ELISA Kit    96 tests                      

EK0335     hFAS-ELISA        人凋亡相關因子酶聯免疫試劑盒         Human FAS ELISA Kit        96 tests                      

EK0337     hFASL-ELISA      人凋亡相關因子配體酶聯免疫試劑盒         Human FASL ELISA Kit      96 tests                      

EK0348     hFGF-9-ELISA   人FGF-9酶聯免疫酶聯免疫試劑盒      Human FGF-9 ELISA Kit    96 tests                      

EK0349     hFN-ELISA         人纖維連接蛋白酶聯免疫試劑盒         Human Fibronectin ELISA Kit  96 tests                      

EK0360     hG-CSF-ELISA   "人粒細胞集落刺激因子酶聯免疫試劑盒

"       Human G-CSF ELISA Kit    96 tests                      

EK0362     hGDNF-ELISA   人神經膠質細胞源性的神經營養因子酶聯免疫試劑盒     Human GDNF ELISA Kit   96 tests                      

EK0364     hGM-CSF-ELISA         "人粒細胞巨噬細胞刺激因子酶聯免疫試劑盒

"       Human GM-CSF ELISA Kit         96 tests                      

EK0369     hHGF-ELISA      人肝細胞生長因子酶聯免疫試劑盒     Human HGF ELISA Kit      96 tests                      

EK0370     hICAM-1-ELISA         人細胞間粘附分子-1酶聯免疫試劑盒         Human ICAM-1 ELISA Kit          96 tests                      

EK0373     hIFN-γ-ELISA  人IFN-γ酶聯免疫試劑盒     Human IFN-γ ELISA Kit 96 tests                      

EK0376     hIGF-1-ELISA    人IGF-1酶聯免疫試劑盒       Human IGF-1 ELISA Kit    96 tests                      

EK0379     hIGF-2-ELISA    人IGF-2酶聯免疫試劑盒       Human IGF-2 ELISA Kit    96 tests                      

EK0382     hIGFBP-ELISA   人IGFBP-1酶聯免疫試劑盒  Human IGFBP-1 ELISA Kit         96 tests                       【豬腦鈉素/腦鈉尿肽(BNP)ELISA 試劑盒現貨】

EK0386     hIGFBP-3-ELISA        人IGFBP-3酶聯免疫試劑盒  Human IGFBP-3 ELISA Kit         96 tests                      

EK0389     hIL-1a-ELISA     人IL-1α酶聯免疫試劑盒      Human IL-1α ELISA Kit 96 tests                      

EK0392     hIL-1α-ELISA  人IL-1α酶聯免疫試劑盒      Human IL-1α ELISA Kit 96 tests                      

EK0397     hIL-2-ELISA       人 IL-2酶聯免疫試劑盒        Human IL-2 ELISA Kit       96 tests                      

EK0402     h IL-3-ELISA      人IL-3 酶聯免疫試劑盒        Human IL-3 ELISA Kit       96 tests                      

EK0404     h IL-4-ELISA      人 IL-4酶聯免疫試劑盒        Human IL-4 ELISA Kit       96 tests                      

EK0407     h IL-5-ELISA      人 IL-5酶聯免疫試劑盒        Human IL-5 ELISA Kit       96 tests                      

EK0410     hIL-6-ELISA       人 IL-6酶聯免疫試劑盒        Human IL-6 ELISA Kit       96 tests                      

EK0413     hIL-8-ELISA       人 IL-8酶聯免疫試劑盒        Human IL-8 ELISA Kit       96 tests