價格詳細資料：試劑盒價格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認同。齊一生物大品牌保證，傾力為國內外科研院校實驗室提供*優質產品。

【兔載脂蛋白A1(apo-A1)ELISA試劑盒現貨】注意事項

1. 當混合蛋白溶液時應盡量輕緩，避免起泡。

2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。

3. 一次加樣時間控制在5分鐘內，如標本數量多，推薦使用排槍加樣。

4. 請每次測定的同時做標準曲線，做復孔。

5. 如標本中待測物質含量過高，請先稀釋后再測定，計算時請*后乘以稀釋倍數。

6. 在配制標準品、檢測溶液工作液時，請以相應的稀釋液配制，不能混淆。

7. 底物請避光保存。

8. 不要用其它生產廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【兔載脂蛋白A1(apo-A1)ELISA試劑盒現貨】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【兔載脂蛋白A1(apo-A1)ELISA試劑盒現貨】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【兔載脂蛋白A1(apo-A1)ELISA試劑盒現貨】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

274302     flocktype IBV Ab (2)          CNY                              【兔載脂蛋白A1(apo-A1)ELISA試劑盒現貨】

274012     flocktype AIV Ab (2)          CNY                             

272702     pigtype PRRSV Ab (2)                 CNY                             

273805     pigtype Yersinia Ab (20)            CNY                             

871101     EGFR PCR Kit (25)              CNY                             

9002244  QIAcube(220-240v) Promo, Priority         QIAcube (220-240V) Promo Priority includes the QIAcube instrument +promo iPad, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.        CNY                             

9018909  Rotor-Disc 100 Loading Block           CNY                             

9019520  ZLV ADAPTER KIT INVITEK                CNY                             

9020574  LED Assy, Green, 15" Long, DML 3000             CNY                             

9020599  Head Alignment Fixture, DML 3000                  CNY                             

9018042  Splash Guard, RCS1           CNY                             

9018190  Tool, arm parallelism, calibration, QIAC           CNY                             

9022614  Ipad 3                CNY                             

9013384  Board, x-main, BR8000             CNY                             

9013248  BGR System Liquid            CNY                             

9013218  ZLV Screws, plastic, M6 (20)             CNY                             

9013217  ZLV Screws, plastic, M5 (20)             CNY                              【兔載脂蛋白A1(apo-A1)ELISA試劑盒現貨】

9013205  BGR Labhand li+re Digene                CNY                             

9013195  Tubing,vac.trap outlet,autov.BR8000v1.00              CNY                             

132572     NeXtal Stock Jeffamine M-600 (50) 100% v/v O-(2-Aminopropyl)-O＇-(2-methoxyethyl)polypropylene 500 - 50mL (5 x 10 ml tube)         CNY                             

132558     NeXtal Stock Lithium sulfate (50)     1M Lithium sulfate monohydrate - 50mL (4 x 12,5 ml tube)   CNY                             

800333     PCR Design                CNY                             

800301     Heterozygote detection, automated      "PCR amplification of genomic DNA

QIAGEN DNA template preparation

Sequencing ａnd sequence alignment

Automatic detection of mutations and

heterozygotic positions

Mutation report

Accuracy >99%"       CNY                             

800251     Single Read HT, w/o prep                  CNY                             

800151     Basic Read Plates              CNY                             

4514263  artus BK Virus RG PCR Kit (24) CE             CNY                             

4513233  artus HI Virus-1 RG RT-PCR Kit (24) RUO                   CNY                             

130710     NeXtal Tubes pHClear II Suite           CNY                             

130707     NeXtal Tubes Anions Suite                 CNY                             

120007     Collection Microtubes ａnd Caps       Nonsterile polypropylene tubes (1.2 ml), 2304 in packs of 96, ａnd nonsterile polypropylene caps for Collection Microtubes          CNY                             

79216       Buffer RLT         220 ml RNeasy Lysis Buffer       CNY                             

76154       RNAlater TissueProtect Tubes (50x1,5ml)       For stabilization of RNA in 50 x 150 mg tissue samples: 50 screw-top tubes containing 1.5 ml RNAlater RNA Stabilization Reagent each CNY                             

QYS-03359        6-磷酸葡萄糖檢測試劑盒      Glucose-6-phosphateAssay      100Tests                             

QYS-03360        葡萄糖檢測試劑盒         GlucoseAssay   100Tests                             

QYS-03361        尿素檢測試劑盒     UricAcidAssay  100Tests                             

QYS-03362        甘油檢測試劑盒     GlycerolAssay  100Tests                             

QYS-03363        "甘油三酸酯檢測試劑盒

"       TriglycerideAssay     100Tests                             

QYS-03364        "總谷胱甘肽檢測試劑盒

"       TotalGlutathioneAssay     100Tests                             

QYS-03365        氨檢測試劑盒         AmmoniaAssay         100Tests                             

QYS-03366        谷胱甘肽/氧化型谷胱甘肽比率檢測試劑盒        GSH/GSSGRatioAssay       100Tests                             

QYS-03367        膽堿檢測試劑盒     CholineAssay    100Tests                             

QYS-03368        線粒體分離試劑盒         MitochondriaIsolationKit         100reactions                               

QYS-03369        細胞色素C氧化酶含量試劑盒     CytochromeCOxidaseAssay      100Testsincuvette                               【兔載脂蛋白A1(apo-A1)ELISA試劑盒現貨】

QYS-03370        檸檬酸合成酶的檢測試劑盒         CitrateSynthaseAssay       100Testsincuvette                              

QYS-03371        一氧化氮色標法檢測試劑盒         ColorimetricNitricOxideAssay  250tests/96wellplate                                   

QYS-03372        總抗氧化能力檢測試劑盒     TotalAntioxidantCapacityAssay        100tests                              

QYS-03373        超氧化物歧化酶分析試劑盒         SuperoxideDisutaseAssay         100tests                              

QYS-03374        過氧化氫酶活性分析試劑盒         CatalaseActivityAssay      100tests                              

QYS-03375        谷胱甘肽過氧化物酶分析試劑盒         GlutathionePeroxidaseAssay   100tests                              

QYS-03376        "NAD/NADH含量檢測試劑盒

"       "NAD/NADHAssay

"       100Testsin96-wellplate                               

QYS-03377        "黃嘌呤氧化酶檢測試劑盒

"       XanthineOxidaseAssay     100Testsin96-wellplat                                 

QYS-03378        總谷胱甘肽含量檢測試劑盒         TotalGlutathioneAssay     100tests                              

QYS-03379        茜素紅S染色試劑盒     AlizarinRedSStainingKit   100ml                                  

QYS-03380        人胚層檢測試劑盒         HumanGermLayerDetectionKit       50reactions                                 

QYS-03381        油紅O染色試劑盒         OilRedOStainingKit  100ml                                  

QYS-03382        人干細胞多能性檢測試劑盒         HumanStemCellPluripotencyDetectionKit       50reactions                                 

QYS-03383        堿性磷酸酶活性測定試劑盒         AlkalinePhosphataseActivityAssay   500tests                              

QYS-03384        堿性磷酸酶染色檢測紅色     AlkalinePhosphataseStainingAssayRed    500tests                              

QYS-03385        人間質干細胞成脂肪細胞檢測試劑盒         HumanMesenchymalStemCellAdipogenesisDetectionKit        50reactions                                     【兔載脂蛋白A1(apo-A1)ELISA試劑盒現貨】

QYS-03386        人間充質干細胞軟骨形成檢測試劑盒         HumanMesenchymalStemCellChondrogenesisDetectionKit   50reactions                                    

QYS-03387        番紅O染色試劑盒         SafraninOStainingKit        100reactions                               

QYS-03388        阿利新藍染色試劑盒     AlcianBlueStainingKit       100reactions