齊一生物試劑盒組分經過重重優化，每個組分的穩定性都完美的經過高溫破壞測驗，程度的避免了長途運輸和保存過程中抗原抗體的失活.齊一生物試劑盒憑借其完美的標曲.嚴格的質控.穩定高、易保存，操作簡便，已廣泛應用在全國免疫學檢驗研究所的各領域中.深受研究者們的青睞. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人Gremlin2蛋白(GREM2)ELISA試劑盒】齊一生物試劑盒憑借其完美的標曲、嚴格的質控、較高的穩定性，在世界范圍內的科研試劑領域中脫穎而出深受研究者們的青睞. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【友情提示】：本產品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

試驗所需自備物品:

1. 酶標儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人Gremlin2蛋白(GREM2)ELISA試劑盒】試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

操作注意事項：

1.試劑盒應按標簽說明書儲存，使用前恢復到室溫30分鐘后方可使用。稀稀過后的標準品應丟棄，不可保存。

2.實驗中不用的板條應立即放回包裝袋中，密封保存，以免變質。

3.不用的其它試劑應包裝好或蓋好。不同批號的試劑不要混用.請在試劑盒保質前使用。

4.使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時，避免使用帶金屬部分的加樣器。

5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。

6.洗滌酶標板時應充分拍干，不要將吸水紙直接放入酶標反應孔中吸水。

7.底物A應揮發，避免長時間打開蓋子。底物B對光敏感，避免長時間暴露于光下。避免用手接觸，有毒。實驗完成后應立即讀取OD值。

8.加入試劑的順序應一致，以保證所有反應板孔溫育的時間一樣。

9.按照說明書中標明的時間、加液的量及順序進行溫育操作。

【人Gremlin2蛋白(GREM2)ELISA試劑盒】齊一生物供應ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據。為了保證實驗數據的可靠性，在實驗過程中必須堅持全面質量控制和全過程質量控制。在收集標本前都必須有一個完整的計劃。

操作步驟
實驗開始前，請提前配置好所有試劑，試劑或樣品稀釋時，均需混勻，混勻時盡量避免起泡。每次檢測都應該做標準曲線。如樣品濃度過高時，用樣品稀釋液進行稀釋，以使樣品符合試劑盒的檢測范圍。
1.         加樣：分別設空白孔、標準孔、待測樣品孔�？瞻卓准訕悠废♂屢�100μl，余孔分別加標準品或待測樣品100μl，注意不要有氣泡，加樣將樣品加于酶標板孔底部，盡量不觸及孔壁，輕輕晃動混勻，酶標板加上蓋或覆膜，37℃反應120分鐘。
為保證實驗結果有效性，每次實驗請使用新的標準品溶液。
2.         棄去液體，甩干，不用洗滌。每孔加生物素標記抗體工作液 100μl（取1μl生物素標記抗體加99μl生物素標記抗體稀釋液的比例配制，輕輕混勻，在使用前一小時內配制），37℃,60分鐘。
3.         溫育60分鐘后，棄去孔內液體，甩干，洗板3次，每次浸泡1-2分鐘，350μl/每孔，甩干。
4.         每孔加辣根過氧化物酶標記親和素工作液（同生物素標記抗體工作液） 100μl，37℃，60分鐘。
5.         溫育60分鐘后，棄去孔內液體，甩干，洗板5次，每次浸泡1-2分鐘，350μl/每孔，甩干。
6.         依序每孔加底物溶液90μl，37℃避光顯色（30分鐘內，此時肉眼可見標準品的前3-4孔有明顯的梯度藍色，后3-4孔梯度不明顯，即可終止）。
7.         依序每孔加終止溶液50μl，終止反應（此時藍色立轉黃色）。終止液的加入順序應盡量與底物液的加入順序相同。為了保證實驗結果的準確性，底物反應時間到后應盡快加入終止液。
8.         用酶聯儀在450nm波長依序測量各孔的光密度（OD值）。 在加終止液后15分鐘以內進行檢測。
【人Gremlin2蛋白(GREM2)ELISA試劑盒】注：
1. 用戶在初次使用試劑盒時，應將各種試劑管離心數分鐘，以便試劑集中到管底。
2. 每次實驗留一孔作為空白調零孔，該孔不加任何試劑，只是*后加底物溶液及2N H2SO4。測量時先用此孔調OD值至零。
3. 為防止樣品蒸發，試驗時將反應板放于鋪有濕布的密閉盒內，酶標板加上蓋或覆膜。
4. 未使用完的酶標板或者試劑，請于2-8℃保存。標準品、生物素標記抗體工作液、辣根過氧化物酶標記親和素工作液請依據所需的量配置使用。請勿重復使用已稀釋過的標準品、生物素標記抗體工作液或、辣根過氧化物酶標記親和素工作液。
5. 建議檢測樣品時均設雙孔測定，以保證檢測結果的準確性。
以上資料僅供您參考，如有問題歡迎來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

74121RNeasy Protect Starter KitRNAlater RNA Stabilization Reagent (20 ml), 6 RNeasy Mini Spin Columns, 2 RNeasy Midi Spin Columns, Collection Tubes (1.5 ａnd 2 ml), RNase-free Reagents ａnd BuffersCNY

74104RNeasy Mini Kit (50)50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd BuffersCNY

72251TurboCapture 96 mRNA Kit (5)For rapid ａnd easy mRNA purification in high-throughput applications. The kit provides a fast ａnd simple procedure for purifying mRNA. Up to 96 samples can be processed simultaneously - ideal for high-throughput applications such as compound screening ａnd target validation (e.g. by siRNA screening).CNY

19407VacConnectors (500)500 disposable connectors for use with QIAamp spin columns on luer connectors. Should be ordered in conjuntion with 19408 - VacValves (24).CNY

12281QIAfilter Plasmid Mega Kit (5)5 QIAGEN-tip 2500, Reagents, Buffers, 5 QIAfilter Mega-Giga CartridgesCNY

12191QIAGEN Plasmid Giga Kit (5)5 QIAGEN-tip 10000, Reagents, BuffersCNY

4502103artus VZV TM PCR Kit (24) RUOCNY

133271NeXtalStock PIPES pH 7.0 (200)CNY

133270NeXtalStock Phosphate-citr. pH 5.2 (200)CNY

990512Filter-Tips, 50 µl (960)CNY

337620qBiomarker SYBR Green Fluor Mastermix(2)CNY

337011qBiomarker Somatic Mutations PCR AssaysCNY

4500263artus HSV-1/2 RG PCR Kit (24) CECNY

939016Buffer ATL ( 4 x 50 ml),GPRCNY

4500163artus HSV-1/2 TM PCR Kit (24) CECNY

1073925Ni-NTA Superflow (8 x 500 ml),cus,GCNY

9010931BGR Greifer P7 (alt)CNY

9010858BGR DT-Abstreifer 8-fach P7xxx DCNY

931036QIAsymphony Virus/Bact Mini Kit (192)CNY

133248NeXtalStock Sodium succinate pH6.5 (200)CNY

330603RT² SYBR Green FAST Mastermix (24)CNY

929608QX Pro Separation Buffer (40 ml)CNY

5000-1415Knob RB671CSKWL Matte, VTX2CNY

5000-1408Cable Shielded 1/17HP, VTX2CNY

L2170hc2 CT/GC HC2 DNA Test PI (CD-ROM)CNY

L1971Rotary Shaker User＇s ManualCNY

9240705O-ring Kit, Capper, Spindle, APCNY

9240693Actril Sterilant & Test Strips, APCNY

9243653Comprehen Valid., Other Extraction Sys"""The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation. 

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   The Customer is expected to have all materials on-hand 1 week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """CNY

386516Investigator STR GO! Lysis Buffer (200)CNY