產品展示
人布氏桿菌�。╞rucellosis）ELISA試劑盒
點擊次數：19發布時間：2016/6/9 21:13:32
更新日期：2016/6/9 21:13:32
所 在 地：中國大陸
產品型號：
優質供應
詳細內容
【友情提示】：本產品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！
本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！
操作步驟
1.準備試劑，樣品和標準品
2.加入準備好的樣品和標準品，37℃反應30分鐘
3.洗板5次，加入酶標試劑，37℃反應30分鐘
4.洗板5次，加入顯色液A、B，37℃顯色15分鐘
5.加入終止液
6.15分鐘之內讀OD值
7.計算
齊一生物供應ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據。為了保證實驗數據的可靠性，在實驗過程中必須堅持全面質量控制和全過程質量控制。在收集標本前都必須有一個完整的計劃。
【人布氏桿菌�。�brucellosis）ELISA試劑盒】樣本保存條件：
1.2-8℃冰箱*多放2周
2.-20到-50℃的冰箱可以放3-6個月
3.-50℃以下的冰箱，可以放一年
【人布氏桿菌�。�brucellosis）ELISA試劑盒】樣本收集
1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集
2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）
3.動物組織勻漿（細胞）：
1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標
1g組織加入1ml組織裂解液加入8ml PBS 測細胞內
糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）
沒有PBS的話也可以用一下生理鹽水，還是用PBS
【人布氏桿菌�。�brucellosis）ELISA試劑盒】樣本離心轉速及時間
1.試管采集的樣本：3000轉，離心五分鐘取上清
2.離心管或者EP管采集的樣本：5000－－8000轉3分鐘取上清
3..樣本保存－－如果樣品不立即使用，應將其分成小部分-70 ℃保存，避免反復冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應在室溫下解凍并確保樣品均勻地充分解凍。
【人布氏桿菌�。�brucellosis）ELISA試劑盒】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490
51162QIAamp 96 DNA Blood Kit (12)For 12 x 96 DNA preps: 12 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection VesselsCNY
50112QIAamp UCP PurePathogen Blood Kit (10)CNY
133104NeXtalStock Sodium acet. (200)CNY
9015468Sensor, photo, PM-L24CNY
9001871EZ1 Advanced, SystemEZ1 Advanced, System combines the EZ1 Advanced system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with a training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.CNY
9018458Screw, w finishing washer (4), QXCNY
9018447Purge Tool, cartridge, QXCNY
9012412Incubator Tower (65°) for Plato 3 holdCNY
9012392Bottle, waste, 10 l, BR8000CNY
205111Omniscript RT Kit (50)For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free waterCNY
9018425Door, sample, QXCNY
9002123QIAxcel Advanced SystemQIAxcel Advanced System combines the QIAxcel Advanced instrument ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It provides the user with training on operation ａnd user maintenance. All travel ａnd labor cost included for Installation. Performance of the installation process is documented on a GMP/GLP-compliant Field Service document.CNY
57714QIAamp MinElute Virus Vacuum Kit (50)For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, Carrier RNA, Buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)CNY
972012PyroMark Q96 CpG p16 (96)For quantification of methylation level in p16CNY
133227NeXtalStock Sodium cacodyl. pH 6.0 (100)CNY
301108PolyFect Transfection Reagent (100 ml)For 2500-6500 transfections in 60 mm dishes or 5000-10,000 transfections in 6-well plates.CNY
56304QIAamp DNA Micro Kit (50)For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)CNY
9002031Rotor-Gene Q MDx 5Plex HRM no PCCNY
9002018Rotor-Gene Q MDx 2plex HRM, PriorityRotor-Gene Q 2plex HRM, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.CNY
970902PyroMark Q24 Advanced Kit (4x24)CNY
133211NeXtalStock Magnesium chloride H2O (200)CNY
133195NeXtalStock Sodium tartrate pH 5.6 (200)CNY
293023mericon Corn (24)CNY
292055mericon Goat Kit (96)CNY
929521QX Alignment Marker 15 bp/1 kb (1.5 ml)CNY
9012598Disposable Filter-Tips, 1100µl (960)Conducting disposable filter-tipsCNY
206152QIAGEN Multiplex PCR Plus Kit (100)CNY
291115mericon RR Soy Kit (96)CNY
291065mericon GMO Screen bar (96)CNY
9001933DML 3000 120-240V QCNY
