1. 酶標儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【友情提示】：本產品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！【大鼠膠原酶II(CollagenaseII)ELISA試劑盒】

ELISA應用：

ELISA檢測試劑盒、ELISAKit、酶聯免疫試劑盒、酶聯免疫吸附測定試劑盒、酶聯免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認可及推崇,在歐美及中國獲得很大的推廣,尤其是國內生化領域的長足發展.Elisa生物試驗是一種敏感性高,特異性強,重復性好的實驗診斷方法.由于其試劑穩定、易保存,操作簡便,結果判斷較客觀等因素,已廣泛應用在免疫學檢驗的各領域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【大鼠膠原酶II(CollagenaseII)ELISA試劑盒】齊一生物銷售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【大鼠膠原酶II(CollagenaseII)ELISA試劑盒】ELISA實驗原理

試劑盒是固相夾心法酶聯免疫吸附實驗（ELISA）.已知濃度的標準品、未知濃度的樣品加入微孔酶標板內進行檢測.先將生物素標記的抗體同時溫育.洗滌后,加入親和素標記過的HRP.再經過溫育和洗滌,去除未結合的酶結合物,然后加入底物A、B,和酶結合物同時作用.產生顏色.顏色的深淺和樣品中的濃度呈比例關系.

免疫測定技術的基礎在于抗原抗體之間的特異結合反應,所以任何優質的診斷試劑離不開優質的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術得到的單克隆抗體,而抗原或抗體的標記物如酶標結合物則通過各種化學合成方法制備.近年來,隨著分子生物學的發展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結合物等免疫測定試劑幾成為現實的新一代試劑,各種新型使用的測定方法也不斷出現.

HBsAg試劑特點（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應性.酶表抗體為與HBsAg有不同結合位點的鼠復合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應用ParlEhrich學說（PEI）HBsAg標準品,對ad標準品的靈敏度為0.05ng/ml對ay標準品靈敏度為0.025ng/ml

ELISA檢測試劑盒應用定性夾心免疫檢測技術,用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標準品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結合物,經第二次孵育后,酶結合物就會與次孵育結合上的HEVIgM抗體相結合,洗板除去未結合的酶結合物,加入TMB底物溶液,在第三次孵育時會發生酶-底物反應,只有那些含有HEVIgM抗體和酶結合物所形成的復合物的孔才會發生顏色變化,加入硫酸溶液終止酶和底物間的反應,并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標準,O.D.值大于或等于Cut-Off值的樣品被認為是初試陽性.

以上資料僅供參考了解，不供于論文寫作

【大鼠膠原酶II(CollagenaseII)ELISA試劑盒】齊一生物專業為您提供高品質，高靈敏度.高質量的酶聯免疫試劑盒，齊一生物長期經營ELISA試劑盒（免費代測）及抗體、分子學生物試劑盒、金標法檢測試劑盒、比色法檢測試劑盒、細胞菌株、培養基、細胞因子、生化試劑、耗材等生物試劑產品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

330502RT² SYBR Green qPCR Mastermix (12)CNY

330401RT² First Strand Kit (12)CNY

9010813BGR Stirrer PK80CNY

69506DNeasy Blood & Tissue Kit (250)250 DNeasy Spin Columns, QIAGEN Proteinase K, Buffers, Collection Tubes (2 ml)CNY

63204DyeEx 2.0 Spin Kit (50)50 DyeEx Spin Columns, Collection Tubes (2 ml)CNY

211392Internal Control DNA (High conc.)CNY

9012755Set, Tubing, Washer, P3CNY

9010126Washer boatCNY

979015MOTT 16S Primer 1 for SequencingCNY

979010PyroMark Vacuum Prep Filter Probe (100)Re-usable filter probes for PyroMark Vacuum Workstation Q96 ａnd Q24.CNY

59720EpiTect Fast 96 DNA Bisulfite KitCNY

9002245QIAcube(220-240v) Promo, PrioPLUSQIAcube (220-240V) Promo Priority includes the QIAcube instrument +promo iPad, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 3 years. One preventive maintenance visit is included for each year of the warranty; 3 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.CNY

9002198RGQ 5plex HRM, PC, PrioPLUS, no PM"RGQ 5plex, HRM, PC Instrument 

Installation of RGQ 5plex HRM & Introductory training 

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

Loaner available in 2 days*

No preventive Maintenance 

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"CNY

9002197RGQ 5plex, PC, PrioPLUS, basic, no PM"RGQ 5plex, PC Instrument 

Installation of RGQ 2plex & Introductory training 

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

No Loaner available 

No Preventive Maintenance 

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"CNY

9012718Pipetting Position (raised platform)CNY

9002042Rotor-Gene Q MDx 6plex PlatformCNY

9002038Rotor-Gene Q MDx 5plex HRM, PriorityRotor-Gene Q MDx 5plex HRM, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.CNY

59110EpiTect 96-well Bisulfite Kit (2)2x EpiTect Bisulfite 96-well Plates, Reaction Mix, DNA Protect Buffer, Carrier RNA, BuffersCNY

133225NeXtalStock Sodium ac. pH 4.8 (200)CNY

301305SuperFect Transfection Reagent (1.2 ml)For 40 transfections in 60 mm dishes or 160 transfections in 12-well platesCNY

970802PyroMark Gold Q24 Reagents (5 x 24)For performing pyrosequncing reaction on the Q24CNY

970470BRAF Pyro Kit (24)CNY

970061MGMT Pyro Kit (48)CNY

133209NeXtalStock MES pH 5.0 (100)CNY

292065mericon Sheep Kit (96)CNY

292045mericon Turkey (96)CNY

929501QX Pro Calibration Marker (0.6 ml)CNY

9012658Cable, X-BCR board to buffer track readeCNY

9012644BGR Nadelrohr P7 StandardnadelnCNY

9012595Disposable Tips, 1100µl (960)Conducting disposable tipsCNY