【友情提示】：本產品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準備試劑，樣品和標準品

2.加入準備好的樣品和標準品，37℃反應30分鐘

3.洗板5次，加入酶標試劑，37℃反應30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內讀OD值

7.計算
齊一生物供應ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據。為了保證實驗數據的可靠性，在實驗過程中必須堅持全面質量控制和全過程質量控制。在收集標本前都必須有一個完整的計劃。

【大鼠游離甲狀腺激素（FT4）Elias試劑盒】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【大鼠游離甲狀腺激素（FT4）Elias試劑盒】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標

              1g組織加入1ml組織裂解液加入8ml PBS 測細胞內

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【大鼠游離甲狀腺激素（FT4）Elias試劑盒】樣本離心轉速及時間

1.試管采集的樣本：3000轉，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應將其分成小部分-70 ℃保存，避免反復冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應在室溫下解凍并確保樣品均勻地充分解凍。

【大鼠游離甲狀腺激素（FT4）Elias試劑盒】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

180850GeneRead qPCR FAST SYBR Green/ROX MM (2)CNY

159300QIAcard FTA Purification Reagent (500ml)CNY

158928Lytic Enzyme Solution (650 µl)CNY

9240549Imbalance PCB, Eppendorf, AP196/98CNY

9240547Motor, 230V, Eppendorf, AP196/98CNY

9240528Cable, Motor, Axis #5, TR, AP98CNY

9240047Thermostate unit, 120V, 1.04CNY

9020029Cable RC-3034, RGQCNY

9020017Oil for PM, EZ1CNY

9020016Cable harness, VacuumStation PCB, CX1CNY

9019938Opto switch, P-axis, 1 ch. , Cx2CNY

9019486HEPA filter, replacement cartridge, CxxCNY

9238861BR 8000, Premium AgreementThe Premium Cover Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).CNY

9238855BR MDx, Basic AgreementThe Basic Cover Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.CNY

9238850EZ1, Basic AgreementThe Basic Cover Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.CNY

9241226Barcode Reader, EZ1aCNY

9238812Rectangular carrier, undrilledCNY

9019467PLATE GLASS SHORT SET GSCNY

9019465Pads, D=10mm, white (85), CxxCNY

9019459Transformer (115V), HSCNY

9233429Valve, magnet, dispenser, BR8000CNY

9233334Solenoid Valve, 5-foldCNY

9237332Install, BR plus 2 integr. w. schedulingInstallation ａnd training, BioRobot System including two approved integration devices with schedulingCNY

9237167Bottom Air FilterCNY

9232994Board, LED, BR8000CNY

9237158RTD Adapter Board XYPCNY

9237152Power InletCNY

9237108Protocol Dev./Opt., on-site (4-days)The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 4 days by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed.CNY

9237098Upgrade QIAsoft, on-siteCNY

9242092QIAgility, Inspection AgreementCNY