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              技術文章

              HLA-G antibody Catalog Number:OM257151

              點擊次數:71 發布時間:2024/9/30 16:26:35
               

              Product Profile

              Product Name HLA-G antibody
              Antibody Type Primary Antibodies
              Product description
              Mouse monoclonal [MEM - G / 1] to HLA GHLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cylasmic tail. [provided by RefSeq]
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              Immunogen
              Recombinant full length denatured heavy chain (Human).

              Key Feature

              Clonality Monoclonal
              Isotype IgG1
              Clone Number MEM-G/1
              Host Species Mouse
              Tested Applications

              IHCIHC-PWB

               
              IHC-P: Use at an assay dependant dilution. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. WB: Use at an assay dependant dilution. Does not react with native HLA-G on cell surface. Optimal dilutions/concentrations should be determined by the end user.:
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              Species Reactivity

              Human

              Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
              Purification Affinity purified

              Target Information

              Gene Synonyms
              HLA G
              HLAG
              MHC-G
              MHC G
              MHCG
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              Target HLA-G
              Tissue Specificity The antibody reacts with denaturated HLA-G heavy chain.
              Cellular Localization Cell Membrane

              Application

              • Application

                Immunohistochemistry staining with anti-human HLA-G (MEM-G/1) . Fig. 1B - first-trimester placenta (paraffin-embedded sections)

              • Application

                The antibody stains infiltrating macrophages in pulmonary diseases. In the left corner see detail of macropage.

              Application Notes
              IHC-P: Use at an assay dependant dilution. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. WB: Use at an assay dependant dilution. Does not react with native HLA-G on cell surface. Optimal dilutions/concentrations should be determined by the end user.:
              More 

              Additional Information

              Form Liquid
              Storage Instructions Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
              Storage Buffer Phosphate buffered saline pH 7.4 containing 15mM sodium azide

              原創作者:上海篤瑪生物科技有限公司

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