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技術文章
HLA-G Antibody Catalog Number：OM281056
Product Profile
| Product Name | HLA-G Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | This HLA-G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-89 amino acids from the Central region of human HLA-G. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | HLA-G |
|---|---|
| Alternative Names | More |
| Molecular Weight(MW) | 38 kDa |
Database Links
| Entrez Gene | 3135 |
|---|---|
| Protein Accession | P17693 |
Application
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Application
Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell ( histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
-
Application
Formalin-fixed ａnd paraffin-embedded human lung carcinoma reacted with HLA-G Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
-
Application
Western blot analysis of HLA-G using rabbit polyclonal HLA-G Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-G gene.
-
Application
Western blot analysis of HLA-G Antibody in NCI-H460 cell line lysates (35ug/lane)
-
Application
Western blot analysis of lysate from human placenta tissue lysate, using HLA-G Antibody ) at 1:1000 at each lane.
-
Application
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lane 3: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.
| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
原創作者：上海篤瑪生物科技有限公司
