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技術文章
SUMO2/3 Antibody (C-term E69) Catalog Number：OM222317
點擊次數：68 發布時間：2024/9/30 16:17:03
Product Profile
| Product Name | SUMO2/3 Antibody (C-term E69) |
|---|---|
| Antibody Type | Primary Antibodies |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB~~1:50~100 IHC~~1:10~50: | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
| Purification |
Target Information
| Gene Synonyms | |
|---|---|
| Alternative Names | More |
| Molecular Weight(MW) | 11637 Da |
| Function | Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation ａnd may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication ａnd repair, mitosis ａnd signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 ａnd linkage to the E2 enzyme UBE2I, ａnd can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4 More |
| Tissue Specificity | Expressed predominantly in liver. |
| Cellular Localization | Cylasm. |
Database Links
| Entrez Gene | 6612 |
|---|
Application
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Application
Western blot analysis of anti-SUMO2/3 Antibody (C-term E69) in CEM cell line lysates (35ug/lane).SUMO2/3(arrow) was detected using the purified Pab.
-
Application
Formalin-fixed ａnd paraffin-embedded human hepatocarcinoma tissue reacted with SUMO2/3 antibody (C-term E69) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Application
Western blot analysis of SUMO2/3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (C-term E69) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2/3 gene.
| Application Notes | WB~~1:50~100 IHC~~1:10~50: |
|---|
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
原創作者：上海篤瑪生物科技有限公司
