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              技術文章

              SUMO2/3 Antibody Catalog Number:OM292543

              點擊次數:52 發布時間:2024/9/30 16:06:20
               

              Product Profile

              Product Name SUMO2/3 Antibody
              Antibody Type Primary Antibodies
              Product description
              SUMO2/3 Antibody: Small ubiquitin-like modifiers (SUMOs) are a family of small, related proteins (Sumo-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, which is a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. All SUMO proteins localize to the nucleus and are covalently conjugated, affecting protein structure, function and interactions. SUMO2 and 3 are 96% identical and are more mobile within nucleus relative to SUMO1. Specific functional differences between SUMO1 and SUMO2 and 3 remain to be identified.1) Kamitani T, Kito K, Nguyen HP, et al. Characterization of a second member of the sentrin family of ubiquitin-like proteins. J. Biol. Chem.1998;273:11349-53.
              More 
              Immunogen
              SUMO2/3 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SUMO2. The immunogen is located within the last 50 amino acids of SUMO2/3.

              Key Feature

              Clonality Polyclonal
              Isotype IgG
              Host Species Rabbit
              Tested Applications

              ELISAIFIHC-PWB

               
              SUMO2/3 antibody can be used for detection of SUMO2 and SUMO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.:
              Species Reactivity

              HumanMouseRat

              Concentration 1 mg/ml
              Purification Affinity purified

              Target Information

              Gene Symbol SUMO2
              Alternative Names
              SUMO2/3 Antibody: HSMT3
              SMT3B
              SUMO3
              Smt3A
              SMT3H2
              SMT3A
              Small ubiquitin-related modifier 2
              HSMT3
              SUMO-2
              More 
              Molecular Weight(MW) Predicted: 15 kDa Observed: 15 kDa
              Sequence Similarities Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (93%), Pig: (93%)

              Database Links

              Entrez Gene 6613
              Protein Accession AAH71645

              Application

              • Application

                Western blot analysis of SUMO2/3 in Jurkat cell lysate with SUMO2/3 antibody at (A) 1 and (B) 2 μg/mL.

              • Application

                Immunofluorescence of SUMO2/3 in rat liver tissue with SUMO2/3 antibody at 20 μg/mL.

              • Application

                Immunohistochemistry of SUMO2/3 in rat liver tissue with SUMO2/3 antibody at 5 μg/mL.

              Positive Control 1) Cat. No. 1205 - Jurkat Cell Lysate
              Application Notes
              SUMO2/3 antibody can be used for detection of SUMO2 and SUMO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.:

              Additional Information

              Form Liquid
              Storage Instructions SUMO2/3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
              Storage Buffer SUMO2/3 Antibody is supplied in PBS containing 0.02% sodium azide.

              原創作者:上海篤瑪生物科技有限公司

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