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              技術文章

              P-V-Myb+C-Myb(S11) [SZ04-81] Catalog Number:OM637105

              點擊次數:43 發布時間:2024/9/30 14:52:58
               

              Product Profile

              Product Name P-V-Myb+C-Myb(S11) [SZ04-81]
              Antibody Type Primary Antibodies
              Product description
              The Myb family of transcription factors regulates proliferation, differentiation and apoptosis of hemaoletic cells. The avian myeloblastosis viral (v-Myb) protein is nuclear and binds to specific DNA sequences. The gene encoding for v-Myb is oncogenic, causing monoblastic leukemia and transforming myelomonocytic cells by deregulating the expression of specific target genes. v-Myb functions as a transcriptional activator, and it can repress biologically relevant genes such as Ets-2, which promotes macrophage differentiation. The proto-oncogene c-Myb encodes for a nuclear protein that plays a role in transcriptional regulation and may be essential for hemaoietic cell proliferation. A-Myb is also member of the Myb family that is expressed in proliferating B cell centroblasts. Transgenic mice overexpressing A-Myb posses enhanced hyperplasia of the lymph nodes.
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              Immunogen
              Synthetic phospho-peptide corresponding to residues surrounding Ser11 of human V-Myb+C-Myb.

              Key Feature

              Clonality Monoclonal
              Isotype IgG
              Host Species Recombinant rabbit
              Tested Applications

              WBICC/IFIHCFC

               
              WB:1:1,000-1:2,000
              ICC:1:50-1:200
              IHC:1:50-1:200
              FC:1:50-1:100
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              Species Reactivity

              Human

              Concentration 1 mg/mL.

              Target Information

              Alternative Names
              C myb antibody C myb protein antibody Cmyb antibody MYB antibody Myb proto oncogene antibody V myb myeloblastosis viral oncogene homolog antibody
              Molecular Weight(MW) 72/75 kDa
              Cellular Localization Nucleus.

              Database Links

              SwissProt ID P10242

              Application

              • Application

                Fig1: ICC staining P-V-Myb+C-Myb(S11) in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

              • Application

                Fig2: Immunohistochemical analysis of paraffin-embedded human uterine neck tissue using anti-P-V-Myb+C-Myb(S11) antibody. Counter stained with hematoxylin.

              • Application

                Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-P-V-Myb+C-Myb(S11) antibody. Counter stained with hematoxylin.

              • Application

                Fig4: Flow cytometric analysis of MCF-7 cells with P-V-Myb+C-Myb(S11) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as antibody

              Positive Control Ags, human uterine neck tissue, human breast carcinoma tissue.
              Application Notes
              WB:1:1,000-1:2,000
              ICC:1:50-1:200
              IHC:1:50-1:200
              FC:1:50-1:100
              More 

              Additional Information

              Form Liquid
              Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
              Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

              原創作者:上海篤瑪生物科技有限公司

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