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              技術文章

              PINCH antibody Catalog Number:OM268604

              點擊次數:34 發布時間:2024/9/29 14:12:14
               

              Product Profile

              Product Name PINCH antibody
              Antibody Type Primary Antibodies
              Product description
              Mouse Monoclonal Antibody [5G7] to PINCHThe protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
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              Immunogen
              The immunogen corresponding to human PINCH expressed in E. Coli.

              Key Feature

              Clonality Monoclonal
              Isotype IgG1
              Clone Number 5G7
              Host Species Mouse
              Tested Applications

              ELISAFACSICC/IFWB

               
              ELISA: 1/10000.:
              Species Reactivity

              Human

              Concentration 1 mg/ml
              Purification  

              Target Information

              Gene Synonyms
              PINCH-1
              LIMS1
              PINCH1
              Target PINCH
              Molecular Weight(MW) 37 kDa (note)

              Application

              • Application

                ICC/IF analysis of HepG2 cells using PINCH antibody [5G7] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).

              • Application

                WB analysis of (1) A549, (2) Jurkat and (3) HeLa lysates using PINCH antibody [5G7].

              • Application

                WB analysis of human PINCH (a.a. 87-249) recombinant protein using PINCH antibody [5G7].

              • Application

                ELISA analysis of control antigen and antigen using PINCH antibody [5G7].

              • Application

                FACS analysis of Hela cells using PINCH antibody [5G7] (blue) or a negative control (red).

              Application Notes
              ELISA: 1/10000.:

              Additional Information

              Form Liquid
              Storage Instructions 4°C; -20°C for long term storage
              Storage Buffer Ascitic fluid containing 0.03% sodium azide.

              原創作者:上海篤瑪生物科技有限公司

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