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技術文章
PINCH [B9-G7] Catalog Number：OM627765
點擊次數：30 發布時間：2024/9/29 14:11:14
Product Profile
| Product Name | PINCH [B9-G7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:1,000 ICC:1:100-1:500 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 2 mg/mL. |
Target Information
| Alternative Names | More |
|---|---|
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Cell junction. |
Database Links
| SwissProt ID | P48059 |
|---|
Application
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Application
Fig1: Western blot analysis of PINCH on human PINCH recombinant protein using anti-PINCH antibody at 1/1,000 dilution.
-
Application
Fig2: Western blot analysis of PINCH on different cell lysates using anti-PINCH antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Jurkat Lane 3: Hela
-
Application
Fig3: ICC staining PINCH (green) ａnd actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Application
Fig4: Flow cytometric analysis of Hela cells with PINCH antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).
| Positive Control | Human PINCH recombinant protein, A549, Jurkat, Hela, HepG2. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 FC:1:100-1:200 |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
原創作者：上海篤瑪生物科技有限公司
