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技術文章
ITK [A6-G12] Catalog Number：OM627718
點擊次數：29 發布時間：2024/9/29 13:58:10
Product Profile
| Product Name | ITK [A6-G12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 2 mg/mL. |
Target Information
| Alternative Names | More |
|---|---|
| Molecular Weight(MW) | 72 kDa |
| Cellular Localization | Cylasm. |
Database Links
| SwissProt ID | Q08881 |
|---|
Application
-
Application
Fig1: Western blot analysis of ITK on human ITK recombinant protein using anti-ITK antibody at 1/1,000 dilution.
-
Application
Fig2: Western blot analysis of ITK on Jurkat cell lysate using anti-ITK antibody at 1/1,000 dilution.
-
Application
Fig3: ICC staining ITK (green) in Jurkat cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Application
Fig4: Flow cytometric analysis of Jurkat cells with ITK antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).
| Positive Control | Human ITK recombinant protein, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:100-1:200 |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
原創作者：上海篤瑪生物科技有限公司
