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              技術文章

              PSMB8 [H2-A5] Catalog Number:OM627709

              點擊次數:50 發布時間:2024/9/27 15:05:27
               

              Product Profile

              Product Name PSMB8 [H2-A5]
              Antibody Type Primary Antibodies
              Product description
              The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
              More 
              Immunogen
              Recombinant protein

              Key Feature

              Clonality Monoclonal
              Isotype IgG1
              Host Species Mouse
              Tested Applications

              WBICCIHC

               
              WB:1:500-1:2,000
              ICC:1:100-1:500
              IHC:1:100-1:500
              Species Reactivity

              HumanRat

              Concentration 2 mg/mL.

              Target Information

              Alternative Names
              ALDD antibody D6S216 antibody D6S216E antibodyLarge multifunctional peptidase 7 antibody Large multifunctional protease 7 antibody LMP 7 antibody LMP7 antibody Low molecular mass protein 7 antibody Low molecular weight protein 7 antibody Macropain subunit C13 antibody MGC1491 antibody Multicatalytic endopeptidase complex subunit C13 antibody NKJO antibody OTTHUMP00000062981 antibody Protease component C13 antibody Proteasome (prosome macropain) subunit beta type 8 antibody Proteasome (prosome
              macropain) subunit
              beta type
              8 (large multifunctional peptidase 7) antibody Proteasome beta 8 subunit antibody Proteasome catalytic subunit 3i antibody Proteasome component C13 antibody Proteasome related gene 7 antibody Proteasome subunit beta 5i antibody Proteasome subunit beta 8 antibody Proteasome subunit beta type 8 antibody Proteasome subunit beta type antibody Proteasome subunit beta type-8 antibody Proteasome subunit beta-5i antibody Proteasome subunit Y2 antibody PSB8_HUMAN antibody PSMB 8 antibody PSMB5i antibody PSMB8 antibody Really interesting new gene 10 protein antibody RING 10 antibody RING10 antibody Y2 antibody
              More 
              Molecular Weight(MW) 22 kDa
              Cellular Localization Cylasm. Nucleus.

              Database Links

              SwissProt ID P28062
              P28064

              Application

              • Application

                Fig1: Western blot analysis of PSMB8 on human PSMB8 recombinant protein using anti-PSMB8 antibody at 1/1,000 dilution.

              • Application

                Fig2: Western blot analysis of PSMB8 on different cell lysates using anti-PSMB8 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: A431 Lane 4: RAJI Lane 5: MOTL4 Lane 6: PC-12

              • Application

                Fig3: ICC staining PSMB8 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

              • Application

                Fig4: Immunohistochemical analysis of paraffin-embedded intima cancer tissue using anti-PSMB8 antibody. Counter stained with hematoxylin.

              • Application

                Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PSMB8 antibody. Counter stained with hematoxylin.

              Application Notes
              WB:1:500-1:2,000
              ICC:1:100-1:500
              IHC:1:100-1:500

              Additional Information

              Form Liquid
              Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
              Storage Buffer 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

              原創作者:上海篤瑪生物科技有限公司

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