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              技術文章

              MCM2 antibody Catalog Number:OM262648

              點擊次數:30 發布時間:2024/9/27 14:44:14
               

              Product Profile

              Product Name MCM2 antibody
              Antibody Type Primary Antibodies
              Product description
              Mouse Monoclonal Antibody [2B3] to MCM2The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]
              More 
              Immunogen
              The immunogen corresponding to human MCM2 expressed in E. Coli.

              Key Feature

              Clonality Monoclonal
              Isotype IgG1
              Clone Number 2B3
              Host Species Mouse
              Tested Applications

              ELISAFACSIHCIHC-PWB

               
              ELISA: 1/10000.:
              Species Reactivity

              HumanMouseMonkeyRat

              Concentration 1 mg/ml
              Purification  

              Target Information

              Gene Synonyms
              CDCL1
              D3S3194
              BM28
              MITOTIN
              CCNL1
              CDC19
              More 
              Target MCM2
              Molecular Weight(MW) 125 kDa (note)

              Application

              • Application

                ELISA analysis of MCM2 antibody [2B3] using control (100 ng) and antigen (10 ng, 50 ng, 100 ng).

              • Application

                WB analysis of PC-12 (1), Cos7 (2), NIH3T3 (3), HepG2 (4), HEK293 (5), K562 (6), Jurkat (7), HeLa (8) and MCF-7 (9) cell lysates using MCM2 antibody [2B3].

              • Application

                FACS analysis of HeLa cells using MCM2 antibody [2B3] (blue) and a negative control (red).

              • Application

                IHC-P analysis of colon cancer tissues using MCM2 antibody [2B3].

              Application Notes
              ELISA: 1/10000.:

              Additional Information

              Form Liquid
              Storage Instructions 4°C; -20°C for long term storage
              Storage Buffer Ascitic fluid containing 0.03% sodium azide.

              原創作者:上海篤瑪生物科技有限公司

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