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技術文章
MCM2 Antibody (Center) Catalog Number：OM213424
點擊次數：33 發布時間：2024/9/27 14:40:42
Product Profile
| Product Name | MCM2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB~~1:50~100 FC~~1:10~50: | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
| Purification |
Target Information
| Gene Synonyms | More |
|---|---|
| Alternative Names | More |
| Molecular Weight(MW) | 101896 Da |
| Function | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for ＇once per cell cycle＇ DNA replication initiation ａnd elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase ａnd for cell division More |
| Tissue Specificity | This MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495~524 amino acids from the Center region of human MCM2. |
| Cellular Localization | Nucleus. |
Database Links
| Entrez Gene | 4171 |
|---|
Application
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Application
Western blot analysis of MCM2 Antibody (Center) in 293 cell line lysates (35ug/lane). MCM2 (arrow) was detected using the purified Pab.
-
Application
MCM2 Antibody (Center) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell ( histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
| Application Notes | WB~~1:50~100 FC~~1:10~50: |
|---|
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
原創作者：上海篤瑪生物科技有限公司
