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              技術文章

              Rabbit anti-MCM2 polyclonal antibody - N-terminal region Catalog Number:OM107926

              點擊次數:30 發布時間:2024/9/27 14:35:49
               

              Product Profile

              Product Name Rabbit anti-MCM2 polyclonal antibody - N-terminal region
              Antibody Type Primary Antibodies
              Immunogen
              The immunogen for anti-MCM2 antibody: synthetic peptide directed towards the N terminal of human MCM2

              Key Feature

              Clonality Polyclonal
              Isotype IgG
              Host Species Rabbit
              Tested Applications

              WB

               
              WB:1:500~1:2000
              Notes:Optimal dilutions/concentrations should be determined by the researcher.
              Species Reactivity

              BovineDogGuinea PigHumanMousePigRabbitRat

              Concentration 1 mg/ml
              Purification Protein A

              Target Information

              Gene Symbol MCM2
              Gene Synonyms
              BM28
              CCNL1
              CDCL1
              cdc19
              D3S3194
              MITOTIN
              More 
              Gene Full Name Minichromosome maintenance complex component 2
              Gene Summary
              The protein encoded by MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
              More 
              Alternative Names
              BM28
              CCNL1
              CDCL1
              cdc19
              D3S3194
              MITOTIN
              More 
              Molecular Weight(MW) 99kDa
              Sequence 904 amino acids

              Database Links

              Entrez Gene 4171
              SwissProt ID P49736
              Protein Accession NP_004517

              Application

              • Western blot

                Positive Control: Lane 1: 5ug mouse neural stem cell lysate
                Primary Antibody Dilution : 1:1000
                Secondary Antibody : Anti rabbit - IR-dye
                Secondry Antibody Dilution : 1:10,000
                Submitted by: Anonymous

              • Western blot

                1.25ug/ml
                ELISA Titer: 1:312500
                Positive Control: Jurkat cell lysate

              Application Notes
              WB:1:500~1:2000
              Notes:Optimal dilutions/concentrations should be determined by the researcher.

              Additional Information

              Form Liquid
              Storage Instructions Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
              Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

              原創作者:上海篤瑪生物科技有限公司

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